Lymphocyte antigen 96

NameLymphocyte antigen 96
Synonyms
  • ESOP-1
  • ESOP1
  • Ly-96
  • MD2
  • Protein MD-2
Gene NameLY96
OrganismHuman
Amino acid sequence
>lcl|BSEQ0037270|Lymphocyte antigen 96
MLPFLFFSTLFSSIFTEAQKQYWVCNSSDASISYTYCDKMQYPISINVNPCIELKRSKGL
LHIFYIPRRDLKQLYFNLYITVNTMNLPKRKEVICRGSDDDYSFCRALKGETVNTTISFS
FKGIKFSKGKYKCVVEAISGSPEEMLFCLEFVILHQPNSN
Number of residues160
Molecular Weight18545.345
Theoretical pI8.46
GO Classification
Functions
  • lipopolysaccharide receptor activity
  • coreceptor activity
Processes
  • toll-like receptor 3 signaling pathway
  • innate immune response
  • toll-like receptor 4 signaling pathway
  • apoptotic process
  • toll-like receptor signaling pathway
  • cell surface receptor signaling pathway
  • toll-like receptor TLR1
  • positive regulation of tumor necrosis factor production
  • toll-like receptor TLR6
  • response to lipopolysaccharide
  • TRIF-dependent toll-like receptor signaling pathway
  • extrinsic apoptotic signaling pathway
  • detection of lipopolysaccharide
  • lipopolysaccharide-mediated signaling pathway
  • activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
  • positive regulation of lipopolysaccharide-mediated signaling pathway
  • cellular defense response
  • I-kappaB kinase/NF-kappaB signaling
  • programmed cell death
  • MyD88-dependent toll-like receptor signaling pathway
  • MyD88-independent toll-like receptor signaling pathway
  • toll-like receptor 2 signaling pathway
  • inflammatory response
Components
  • intrinsic component of plasma membrane
  • lipopolysaccharide receptor complex
  • endosome membrane
  • plasma membrane
  • extracellular space
General FunctionLipopolysaccharide receptor activity
Specific FunctionBinds bacterial lipopolysaccharide (LPS) (PubMed:17803912, PubMed:17569869). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria (PubMed:11160242, PubMed:11593030). Enhances TLR4-dependent activation of NF-kappa-B (PubMed:10359581). Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS (PubMed:10359581).
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID5051740
UniProtKB IDQ9Y6Y9
UniProtKB Entry NameLY96_HUMAN
Cellular LocationSecreted
Gene sequence
>lcl|BSEQ0021698|Lymphocyte antigen 96 (LY96)
ATGTTACCATTTCTGTTTTTTTCCACCCTGTTTTCTTCCATATTTACTGAAGCTCAGAAG
CAGTATTGGGTCTGCAACTCATCCGATGCAAGTATTTCATACACCTACTGTGGGAGAGAT
TTAAAGCAATTATATTTCAATCTCTATATAACTGTCAACACCATGAATCTTCCAAAGCGC
AAAGAAGTTATTTGCCGAGGATCTGATGACGATTACTCTTTTTGCAGAGCTCTGAAGGGA
GAGACTGTGAATACAACAATATCATTCTCCTTCAAGGGAATAAAATTTTCTAAGGGAAAA
TACAAATGTGTTGTTGAAGCTATTTCTGGGAGCCCAGAAGAAATGCTCTTTTGCTTGGAG
TTTGTCATCCTACACCAACCTAATTCAAATTAG
GenBank Gene IDAB018549
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:17156
Chromosome Location8
Locus8q21.11
References
  1. Shimazu R, Akashi S, Ogata H, Nagai Y, Fukudome K, Miyake K, Kimoto M: MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med. 1999 Jun 7;189(11):1777-82. 10359581
  2. Kato K, Morrison AM, Nakano T, Tashiro K, Honjo T: ESOP-1, a secreted protein expressed in the hematopoietic, nervous, and reproductive systems of embryonic and adult mice. Blood. 2000 Jul 1;96(1):362-4. 10891475
  3. Schromm AB, Lien E, Henneke P, Chow JC, Yoshimura A, Heine H, Latz E, Monks BG, Schwartz DA, Miyake K, Golenbock DT: Molecular genetic analysis of an endotoxin nonresponder mutant cell line: a point mutation in a conserved region of MD-2 abolishes endotoxin-induced signaling. J Exp Med. 2001 Jul 2;194(1):79-88. 11435474
  4. Nakajima T, Ohtani H, Satta Y, Uno Y, Akari H, Ishida T, Kimura A: Natural selection in the TLR-related genes in the course of primate evolution. Immunogenetics. 2008 Dec;60(12):727-35. doi: 10.1007/s00251-008-0332-0. Epub 2008 Sep 23. 18810425
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  7. da Silva Correia J, Soldau K, Christen U, Tobias PS, Ulevitch RJ: Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem. 2001 Jun 15;276(24):21129-35. Epub 2001 Mar 26. 11274165
  8. Dziarski R, Wang Q, Miyake K, Kirschning CJ, Gupta D: MD-2 enables Toll-like receptor 2 (TLR2)-mediated responses to lipopolysaccharide and enhances TLR2-mediated responses to Gram-positive and Gram-negative bacteria and their cell wall components. J Immunol. 2001 Feb 1;166(3):1938-44. 11160242
  9. Visintin A, Mazzoni A, Spitzer JA, Segal DM: Secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to Toll-like receptor 4. Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):12156-61. Epub 2001 Oct 2. 11593030
  10. Mullen GE, Kennedy MN, Visintin A, Mazzoni A, Leifer CA, Davies DR, Segal DM: The role of disulfide bonds in the assembly and function of MD-2. Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3919-24. Epub 2003 Mar 17. 12642668
  11. Kim HM, Park BS, Kim JI, Kim SE, Lee J, Oh SC, Enkhbayar P, Matsushima N, Lee H, Yoo OJ, Lee JO: Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran. Cell. 2007 Sep 7;130(5):906-17. 17803912
  12. Ohto U, Fukase K, Miyake K, Satow Y: Crystal structures of human MD-2 and its complex with antiendotoxic lipid IVa. Science. 2007 Jun 15;316(5831):1632-4. 17569869