Protein phosphatase 1 regulatory subunit 14A

NameProtein phosphatase 1 regulatory subunit 14A
Synonyms
  • 17 kDa PKC-potentiated inhibitory protein of PP1
  • CPI-17
  • CPI17
  • PPP1INL
  • Protein kinase C-potentiated inhibitor protein of 17 kDa
Gene NamePPP1R14A
OrganismHuman
Amino acid sequence
>lcl|BSEQ0009118|Protein phosphatase 1 regulatory subunit 14A
MAAQRLGKRVLSKLQSPSRARGPGGSPGGLQKRHARVTVKYDRRELQRRLDVEKWIDGRL
EELYRGMEADMPDEINIDELLELESEEERSRKIQGLLKSCGKPVEDFIQELLAKLQGLHR
QPGLRQPSPSHDGSLSPLQDRARTAHP
Number of residues147
Molecular Weight16692.84
Theoretical pINot Available
GO Classification
Functions
  • protein phosphatase inhibitor activity
Processes
  • regulation of phosphorylation
  • small GTPase mediated signal transduction
Components
  • cytosol
General FunctionProtein phosphatase inhibitor activity
Specific FunctionInhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDQ96A00
UniProtKB Entry NamePP14A_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0017694|Protein phosphatase 1 regulatory subunit 14A (PPP1R14A)
ATGGCAGCTCAGCGGCTGGGCAAGCGCGTGCTGAGCAAGCTGCAGTCTCCATCGCGGGCC
CGCGGGCCAGGGGGCAGTCCCGGGGGGCTGCAGAAGCGGCACGCGCGCGTCACCGTCAAG
TATGACCGGCGGGAGCTGCAGCGGCGGCTGGACGTGGAGAAGTGGATCGACGGGCGCCTG
GAGGAGCTGTACCGCGGCATGGGACTCCTGAAGTCATGTGGGAAACCTGTCGAGGACTTC
ATCCAGGAGCTGCTGGCAAAGCTTCAAGGCCTCCACAGGCAGCCCGGCCTCCGCCAGCCA
AGCCCCTCCCACGACGGCAGCCTCAGCCCCCTCCAGGACCGGGCCCGGACTGCTCACCCC
TGA
GenBank Gene IDNot Available
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:14871
Chromosome Location19
LocusNot Available
References
  1. Yamawaki K, Ito M, Machida H, Moriki N, Okamoto R, Isaka N, Shimpo H, Kohda A, Okumura K, Hartshorne DJ, Nakano T: Identification of human CPI-17, an inhibitory phosphoprotein for myosin phosphatase. Biochem Biophys Res Commun. 2001 Jul 27;285(4):1040-5. 11467857
  2. Liu QR, Zhang PW, Lin Z, Li QF, Woods AS, Troncoso J, Uhl GR: GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein, is activated by PKC and inactivated by PKA. Biochem J. 2004 Jan 1;377(Pt 1):171-81. 12974676
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  4. Zemlickova E, Johannes FJ, Aitken A, Dubois T: Association of CPI-17 with protein kinase C and casein kinase I. Biochem Biophys Res Commun. 2004 Mar 26;316(1):39-47. 15003508
  5. Zahedi RP, Lewandrowski U, Wiesner J, Wortelkamp S, Moebius J, Schutz C, Walter U, Gambaryan S, Sickmann A: Phosphoproteome of resting human platelets. J Proteome Res. 2008 Feb;7(2):526-34. Epub 2007 Dec 19. 18088087
  6. Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17. 21269460
  7. Rigbolt KT, Prokhorova TA, Akimov V, Henningsen J, Johansen PT, Kratchmarova I, Kassem M, Mann M, Olsen JV, Blagoev B: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal. 2011 Mar 15;4(164):rs3. doi: 10.1126/scisignal.2001570. 21406692
  8. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. 24275569