Hypoxia-inducible factor 1-alpha

NameHypoxia-inducible factor 1-alpha
Synonyms
  • ARNT-interacting protein
  • Basic-helix-loop-helix-PAS protein MOP1
  • BHLHE78
  • Class E basic helix-loop-helix protein 78
  • HIF-1-alpha
  • Member of PAS protein 1
  • MOP1
  • PAS domain-containing protein 8
  • PASD8
Gene NameHIF1A
OrganismHuman
Amino acid sequence
>lcl|BSEQ0004663|Hypoxia-inducible factor 1-alpha
MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVM
RLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYM
GLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGR
TMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSK
TFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQV
TTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECV
LKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTET
DDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEP
NPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLF
AEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVT
VFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYR
DTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKR
KMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLAC
RLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN
Number of residues826
Molecular Weight92669.595
Theoretical pI4.97
GO Classification
Functions
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
  • transcription factor activity, RNA polymerase II transcription factor binding
  • transcription factor activity, sequence-specific DNA binding
  • protein heterodimerization activity
  • transcription factor activity, transcription factor binding
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
  • transcription factor binding
  • protein kinase binding
  • nuclear hormone receptor binding
  • histone acetyltransferase binding
  • enzyme binding
  • ubiquitin protein ligase binding
  • sequence-specific DNA binding
  • Hsp90 protein binding
Processes
  • negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway
  • negative regulation of mesenchymal cell apoptotic process
  • lactation
  • regulation of transforming growth factor beta2 production
  • positive regulation of nitric-oxide synthase activity
  • retina vasculature development in camera-type eye
  • hemoglobin biosynthetic process
  • cellular iron ion homeostasis
  • collagen metabolic process
  • vascular endothelial growth factor production
  • cellular response to hypoxia
  • axon transport of mitochondrion
  • glucose homeostasis
  • embryonic placenta development
  • oxygen homeostasis
  • positive regulation of angiogenesis
  • positive regulation of transcription from RNA polymerase II promoter in response to hypoxia
  • negative regulation of TOR signaling
  • regulation of transcription from RNA polymerase II promoter in response to hypoxia
  • positive regulation of chemokine production
  • positive regulation of pri-miRNA transcription from RNA polymerase II promoter
  • regulation of transcription, DNA-templated
  • epithelial to mesenchymal transition
  • positive regulation of receptor biosynthetic process
  • positive regulation of vascular endothelial growth factor production
  • regulation of aerobic respiration
  • positive regulation of transcription, DNA-templated
  • negative regulation of growth
  • neural crest cell migration
  • positive regulation of transcription from RNA polymerase II promoter
  • negative regulation of bone mineralization
  • outflow tract morphogenesis
  • heart looping
  • positive regulation of endothelial cell proliferation
  • positive regulation of hormone biosynthetic process
  • response to hypoxia
  • muscle cell cellular homeostasis
  • mRNA transcription from RNA polymerase II promoter
  • digestive tract morphogenesis
  • regulation of gene expression
  • cerebral cortex development
  • hypoxia-inducible factor-1alpha signaling pathway
  • Notch signaling pathway
  • positive regulation of chemokine-mediated signaling pathway
  • connective tissue replacement involved in inflammatory response wound healing
  • positive regulation of erythrocyte differentiation
  • positive regulation of vascular endothelial growth factor receptor signaling pathway
  • regulation of transcription from RNA polymerase II promoter in response to oxidative stress
  • lactate metabolic process
  • positive regulation of epithelial cell migration
  • signal transduction
  • visual learning
  • B-1 B cell homeostasis
  • angiogenesis
  • dopaminergic neuron differentiation
  • positive regulation of glycolytic process
  • positive regulation of mitophagy
  • cardiac ventricle morphogenesis
  • embryonic hemopoiesis
  • cellular response to interleukin-1
  • negative regulation of thymocyte apoptotic process
  • cartilage development
  • response to muscle activity
  • elastin metabolic process
  • intestinal epithelial cell maturation
  • positive regulation of insulin secretion involved in cellular response to glucose stimulus
  • epithelial cell differentiation involved in mammary gland alveolus development
  • positive regulation of neuroblast proliferation
  • negative regulation of reactive oxygen species metabolic process
  • neural fold elevation formation
Components
  • cytosol
  • nucleoplasm
  • nuclear speck
  • cytoplasm
  • nucleus
  • transcription factor complex
  • RNA polymerase II transcription factor complex
  • motile cilium
General FunctionUbiquitin protein ligase binding
Specific FunctionFunctions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDQ16665
UniProtKB Entry NameHIF1A_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0021673|Hypoxia-inducible factor 1-alpha (HIF1A)
ATGAGCTCCCAATGTCGGAGTTTGGAAAACAAATTTGTCTTTTTAAAAGAAGGTCTAGGA
AACTCAAAACCTGAAGAATTGGAAGAAATCAGAATAGAAAATGGTAGGATAAGTTCTGAA
CGTCGAAAAGAAAAGTCTCGAGATGCAGCCAGATCTCGGCGAAGTAAAGAATCTGAAGTT
TTTTATGAGCTTGCTCATCAGTTGCCACTTCCACATAATGTGAGTTCGCATCTTGATAAG
GCCTCTGTGATGAGGCTTACCATCAGCTATTTGCGTGTGAGGAAACTTCTGGATGCTGGT
GATTTGGATATTGAAGATGACATGAAAGCACAGATGAATTGCTTTTATTTGAAAGCCTTG
GATGGTTTTGTTATGGTTCTCACAGATGATGGTGACATGATTTACATTTCTGATAATGTG
AACAAATACATGGGATTAACTCAGTTTGAACTAACTGGACACAGTGTGTTTGATTTTACT
CATCCATGTGACCATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCTTGTGAAA
AAGGGTAAAGAACAAAACACACAGCGAAGCTTTTTTCTCAGAATGAAGTGTACCCTAACT
AGCCGAGGAAGAACTATGAACATAAAGTCTGCAACATGGAAGGTATTGCACTGCACAGGC
CACATTCACGTATATGATACCAACAGTAACCAACCTCAGTGTGGGTATAAGAAACCACCT
ATGACCTGCTTGGTGCTGATTTGTGAACCCATTCCTCACCCATCAAATATTGAAATTCCT
TTAGATAGCAAGACTTTCCTCAGTCGACACAGCCTGGATATGAAATTTTCTTATTGTGAT
GAAAGAATTACCGAATTGATGGGATATGAGCCAGAAGAACTTTTAGGCCGCTCAATTTAT
GAATATTATCATGCTTTGGACTCTGATCATCTGACCAAAACTCATCATGATATGTTTACT
AAAGGACAAGTCACCACAGGACAGTACAGGATGCTTGCCAAAAGAGGTGGATATGTCTGG
GTTGAAACTCAAGCAACTGTCATATATAACACCAAGAATTCTCAACCACAGTGCATTGTA
TGTGTGAATTACGTTGTGAGTGGTATTATTCAGCACGACTTGATTTTCTCCCTTCAACAA
ACAGAATGTGTCCTTAAACCGGTTGAATCTTCAGATATGAAAATGACTCAGCTATTCACC
AAAGTTGAATCAGAAGATACAAGTAGCCTCTTTGACAAACTTAAGAAGGAACCTGATGCT
TTAACTTTGCTGGCCCCAGCCGCTGGAGACACAATCATATCTTTAGATTTTGGCAGCAAC
GACACAGAAACTGATGACCAGCAACTTGAGGAAGTACCATTATATAATGATGTAATGCTC
CCCTCACCCAACGAAAAATTACAGAATATAAATTTGGCAATGTCTCCATTACCCACCGCT
GAAACGCCAAAGCCACTTCGAAGTAGTGCTGACCCTGCACTCAATCAAGAAGTTGCATTA
AAATTAGAACCAAATCCAGAGTCACTGGAACTTTCTTTTACCATGCCCCAGATTCAGGAT
CAGACACCTAGTCCTTCCGATGGAAGCACTAGACAAAGTTCACCTGAGCCTAATAGTCCC
AGTGAATATTGTTTTTATGTGGATAGTGATATGGTCAATGAATTCAAGTTGGAATTGGTA
GAAAAACTTTTTGCTGAAGACACAGAAGCAAAGAACCCATTTTCTACTCAGGACACAGAT
TTAGACTTGGAGATGTTAGCTCCCTATATCCCAATGGATGATGACTTCCAGTTACGTTCC
TTCGATCAGTTGTCACCATTAGAAAGCAGTTCCGCAAGCCCTGAAAGCGCAAGTCCTCAA
AGCACAGTTACAGTATTCCAGCAGACTCAAATACAAGAACCTACTGCTAATGCCACCACT
ACCACTGCCACCACTGATGAATTAAAAACAGTGACAAAAGACCGTATGGAAGACATTAAA
ATATTGATTGCATCTCCATCTCCTACCCACATACATAAAGAAACTACTAGTGCCACATCA
TCACCATATAGAGATACTCAAAGTCGGACAGCCTCACCAAACAGAGCAGGAAAAGGAGTC
ATAGAACAGACAGAAAAATCTCATCCAAGAAGCCCTAACGTGTTATCTGTCGCTTTGAGT
CAAAGAACTACAGTTCCTGAGGAAGAACTAAATCCAAAGATACTAGCTTTGCAGAATGCT
CAGAGAAAGCGAAAAATGGAACATGATGGTTCACTTTTTCAAGCAGTAGGAATTGGAACA
TTATTACAGCAGCCAGACGATCATGCAGCTACTACATCACTTTCTTGGAAACGTGTAAAA
GGATGCAAATCTAGTGAACAGAATGGAATGGAGCAAAAGACAATTATTTTAATACCCTCT
GATTTAGCATGTAGACTGCTGGGGCAATCAATGGATGAAAGTGGATTACCACAGCTGACC
AGTTATGATTGTGAAGTTAATGCTCCTATACAAGGCAGCAGAAACCTACTGCAGGGTGAA
GAATTACTCAGAGCTTTGGATCAAGTTAACTGA
GenBank Gene IDU22431
GeneCard IDNot Available
GenAtlas IDHIF1A
HGNC IDHGNC:4910
Chromosome Location14
LocusNot Available
References
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