Caspase-8

NameCaspase-8
Synonyms
  • 3.4.22.61
  • Apoptotic cysteine protease
  • Apoptotic protease Mch-5
  • CAP4
  • CASP-8
  • FADD-homologous ICE/ced-3-like protease
  • FADD-like ICE
  • FLICE
  • ICE-like apoptotic protease 5
  • MACH
  • MCH5
  • MORT1-associated ced-3 homolog
Gene NameCASP8
OrganismHuman
Amino acid sequence
>lcl|BSEQ0006731|Caspase-8
MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLS
FLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSF
KFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEE
FSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIIN
NHNFAKAREKVPKLHSIRDRNGTHLDAGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQ
LMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQAC
QGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTW
YIQSLCQSLRERCPRGDDILTILTEVNYEVSNKDDKKNMGKQMPQPTFTLRKKLVFPSD
Number of residues479
Molecular Weight55390.53
Theoretical pI4.73
GO Classification
Functions
  • cysteine-type endopeptidase activity involved in apoptotic process
  • death effector domain binding
  • peptidase activity
  • cysteine-type endopeptidase activity involved in apoptotic signaling pathway
  • cysteine-type endopeptidase activity
  • cysteine-type peptidase activity
  • ubiquitin protein ligase binding
  • scaffold protein binding
Processes
  • natural killer cell activation
  • protein heterooligomerization
  • innate immune response
  • negative regulation of I-kappaB kinase/NF-kappaB signaling
  • response to ethanol
  • proteolysis involved in cellular protein catabolic process
  • cellular response to mechanical stimulus
  • response to estradiol
  • B cell activation
  • regulation of thymocyte apoptotic process
  • apoptotic signaling pathway
  • positive regulation of extrinsic apoptotic signaling pathway
  • apoptotic process
  • MyD88-independent toll-like receptor signaling pathway
  • syncytiotrophoblast cell differentiation involved in labyrinthine layer development
  • T cell activation
  • activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
  • death-inducing signaling complex assembly
  • toll-like receptor 3 signaling pathway
  • TRAIL-activated apoptotic signaling pathway
  • activation of cysteine-type endopeptidase activity involved in apoptotic process
  • programmed necrotic cell death
  • heart development
  • toll-like receptor 4 signaling pathway
  • intrinsic apoptotic signaling pathway
  • regulation of extrinsic apoptotic signaling pathway via death domain receptors
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
  • toll-like receptor signaling pathway
  • positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
  • regulation of necrotic cell death
  • stimulatory C-type lectin receptor signaling pathway
  • TRIF-dependent toll-like receptor signaling pathway
  • proteolysis
  • response to cobalt ion
  • positive regulation of proteolysis
  • cell surface receptor signaling pathway
  • extrinsic apoptotic signaling pathway
  • response to antibiotic
  • macrophage differentiation
  • hepatocyte apoptotic process
  • cellular component disassembly involved in execution phase of apoptosis
  • programmed cell death
  • response to cold
  • viral process
  • execution phase of apoptosis
  • neural tube formation
  • response to lipopolysaccharide
  • response to tumor necrosis factor
  • angiogenesis
  • positive regulation of macrophage differentiation
  • tumor necrosis factor-mediated signaling pathway
  • nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway
  • negative regulation of necroptotic process
  • activation of cysteine-type endopeptidase activity
  • nucleotide-binding oligomerization domain containing signaling pathway
  • necroptotic process
  • cellular response to organic cyclic compound
  • positive regulation of I-kappaB kinase/NF-kappaB signaling
  • regulation of tumor necrosis factor-mediated signaling pathway
Components
  • Noc1p-Noc2p complex
  • cytoplasm
  • mitochondrion
  • cytoskeleton
  • membrane raft
  • neuron projection
  • microtubule organizing center
  • death-inducing signaling complex
  • mitochondrial outer membrane
  • ripoptosome
  • cell body
  • cytosol
  • CD95 death-inducing signaling complex
  • nucleoplasm
General FunctionUbiquitin protein ligase binding
Specific FunctionMost upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDQ14790
UniProtKB Entry NameCASP8_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0019424|Caspase-8 (CASP8)
ATGGACTTCAGCAGAAATCTTTATGATATTGGGGAACAACTGGACAGTGAAGATCTGGCC
TCCCTCAAGTTCCTGAGCCTGGACTACATTCCGCAAAGGAAGCAAGAACCCATCAAGGAT
GCCTTGATGTTATTCCAGAGACTCCAGGAAAAGAGAATGTTGGAGGAAAGCAATCTGTCC
TTCCTGAAGGAGCTGCTCTTCCGAATTAATAGACTGGATTTGCTGATTACCTACCTAAAC
ACTAGAAAGGAGGAGATGGAAAGGGAACTTCAGACACCAGGCAGGGCTCAAATTTCTGCC
TACAGGGTCATGCTCTATCAGATTTCAGAAGAAGTGAGCAGATCAGAATTGAGGTCTTTT
AAGTTTCTTTTGCAAGAGGAAATCTCCAAATGCAAACTGGATGATGACATGAACCTGCTG
GATATTTTCATAGAGATGGAGAAGAGGGTCATCCTGGGAGAAGGAAAGTTGGACATCCTG
AAAAGAGTCTGTGCCCAAATCAACAAGAGCCTGCTGAAGATAATCAACGACTATGAAGAA
TTCAGCAAAGGGGAGGAGTTGTGTGGGGTAATGACAATCTCGGACTCTCCAAGAGAACAG
GATAGTGAATCACAGACTTTGGACAAAGTTTACCAAATGAAAAGCAAACCTCGGGGATAC
TGTCTGATCATCAACAATCACAATTTTGCAAAAGCACGGGAGAAAGTGCCCAAACTTCAC
AGCATTAGGGACAGGAATGGAACACACTTGGATGCAGGGGCTTTGACCACGACCTTTGAA
GAGCTTCATTTTGAGATCAAGCCCCACGATGACTGCACAGTAGAGCAAATCTATGAGATT
TTGAAAATCTACCAACTCATGGACCACAGTAACATGGACTGCTTCATCTGCTGTATCCTC
TCCCATGGAGACAAGGGCATCATCTATGGCACTGATGGACAGGAGGCCCCCATCTATGAG
CTGACATCTCAGTTCACTGGTTTGAAGTGCCCTTCCCTTGCTGGAAAACCCAAAGTGTTT
TTTATTCAGGCTTGTCAGGGGGATAACTACCAGAAAGGTATACCTGTTGAGACTGATTCA
GAGGAGCAACCCTATTTAGAAATGGATTTATCATCACCTCAAACGAGATATATCCCGGAT
GAGGCTGACTTTCTGCTGGGGATGGCCACTGTGAATAACTGTGTTTCCTACCGAAACCCT
GCAGAGGGAACCTGGTACATCCAGTCACTTTGCCAGAGCCTGAGAGAGCGATGTCCTCGA
GGCGATGATATTCTCACCATCCTGACTGAAGTGAACTATGAAGTAAGCAACAAGGATGAC
AAGAAAAACATGGGGAAACAGATGCCTCAGCCTACTTTCACACTAAGAAAAAAACTTGTC
TTCCCTTCTGATTGA
GenBank Gene IDAC007256
GeneCard IDNot Available
GenAtlas IDCASP8
HGNC IDHGNC:1509
Chromosome Location2
LocusNot Available
References
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