S-methyl-5'-thioadenosine phosphorylase

NameS-methyl-5'-thioadenosine phosphorylase
Synonyms
  • 2.4.2.28
  • 5'-methylthioadenosine phosphorylase
  • MSAP
  • MTA phosphorylase
  • MTAP
  • MTAPase
Gene NameMTAP
OrganismHuman
Amino acid sequence
>lcl|BSEQ0002244|S-methyl-5'-thioadenosine phosphorylase
MASGTTTTAVKIGIIGGTGLDDPEILEGRTEKYVDTPFGKPSDALILGKIKNVDCVLLAR
HGRQHTIMPSKVNYQANIWALKEEGCTHVIVTTACGSLREEIQPGDIVIIDQFIDRTTMR
PQSFYDGSHSCARGVCHIPMAEPFCPKTREVLIETAKKLGLRCHSKGTMVTIEGPRFSSR
AESFMFRTWGADVINMTTVPEVVLAKEAGICYASIAMATDYDCWKEHEEAVSVDRVLKTL
KENANKAKSLLLTTIPQIGSTEWSETLHNLKNMAQFSVLLPRH
Number of residues283
Molecular Weight31235.76
Theoretical pI7.21
GO Classification
Functions
  • phosphorylase activity
  • S-methyl-5-thioadenosine phosphorylase activity
Processes
  • purine ribonucleoside salvage
  • cellular nitrogen compound metabolic process
  • small molecule metabolic process
  • purine-containing compound salvage
  • polyamine metabolic process
  • sulfur amino acid metabolic process
  • nucleobase-containing compound metabolic process
  • L-methionine biosynthetic process from methylthioadenosine
Components
  • cytoplasm
  • nucleus
  • cytosol
  • extracellular exosome
General FunctionS-methyl-5-thioadenosine phosphorylase activity
Specific FunctionCatalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID847724
UniProtKB IDQ13126
UniProtKB Entry NameMTAP_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0010787|S-methyl-5'-thioadenosine phosphorylase (MTAP)
ATGGCCTCTGGCACCACCACCACCGCCGTGAAGATTGGAATAATTGGTGGAACAGGCCTG
GATGATCCAGAAATTTTAGAAGGAAGAACTGAAAAATATGTGGATACTCCATTTGGCAAG
CCATCTGATGCCTTAATTTTGGGGAAGATAAAAAATGTTGATTGCGTCCTCCTTGCAAGG
CATGGAAGGCAGCACACCATCATGCCTTCAAAGGTCAACTACCAGGCGAACATCTGGGCT
TTGAAGGAAGAGGGCTGTACACATGTCATAGTGACCACAGCTTGTGGCTCCTTGAGGGAG
GAGATTCAGCCCGGCGATATTGTCATTATTGATCAGTTCATTGACAGGACCACTATGAGA
CCTCAGTCCTTCTATGATGGAAGTCATTCTTGTGCCAGAGGAGTGTGCCATATTCCAATG
GCTGAGCCGTTTTGCCCCAAAACGAGAGAGGTTCTTATAGAGACTGCTAAGAAGCTAGGA
CTCCGGTGCCACTCAAAGGGGACAATGGTCACAATCGAGGGACCTCGTTTTAGCTCCCGG
GCAGAAAGCTTCATGTTCCGCACCTGGGGGGCGGATGTTATCAACATGACCACAGTTCCA
GAGGTGGTTCTTGCTAAGGAGGCTGGAATTTGTTACGCAAGTATCGCCATGGCGACAGAT
TATGACTGCTGGAAGGAGCACGAGGAAGCAGTTTCGGTGGACCGGGTCTTAAAGACCCTG
AAAGAAAACGCTAATAAAGCCAAAAGCTTACTGCTCACTACCATACCTCAGATAGGGTCC
ACAGAATGGTCAGAAACCCTCCATAACCTGAAGAATATGGCCCAGTTTTCTGTTTTATTA
CCAAGACATTAA
GenBank Gene IDU22233
GeneCard IDNot Available
GenAtlas IDMTAP
HGNC IDHGNC:7413
Chromosome Location9
Locus9p21
References
  1. Olopade OI, Pomykala HM, Hagos F, Sveen LW, Espinosa R 3rd, Dreyling MH, Gursky S, Stadler WM, Le Beau MM, Bohlander SK: Construction of a 2.8-megabase yeast artificial chromosome contig and cloning of the human methylthioadenosine phosphorylase gene from the tumor suppressor region on 9p21. Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6489-93. 7604019
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  6. Della Ragione F, Carteni-Farina M, Gragnaniello V, Schettino MI, Zappia V: Purification and characterization of 5'-deoxy-5'-methylthioadenosine phosphorylase from human placenta. J Biol Chem. 1986 Sep 15;261(26):12324-9. 3091600
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