Transcriptional repressor NF-X1

NameTranscriptional repressor NF-X1
Synonyms
  • 6.3.2.-
  • NFX2
  • Nuclear transcription factor, X box-binding protein 1
Gene NameNFX1
OrganismHuman
Amino acid sequence
>lcl|BSEQ0009240|Transcriptional repressor NF-X1
MAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQV
PYDEISAVHQHSYHPSGSKPKSQQTSFQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKK
AQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVYSYGR
GPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRR
YPQKRPPWEVEGARPRPGRNPPKQEGHRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENL
AVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKYECMV
CCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYT
CFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECG
RTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGSTSRDV
LCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTP
LSQLLELGSSSRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVIS
CRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLR
CGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDH
PVYHSCHSEEKCPPCTFLTQKWCMGKHEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRL
CHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKVELQCECGRRKEMVI
CSEASSTYQRIAAISMASKITDMQLGGSVEISKLITKKEVHQARLECDEECSALERKKRL
AEAFHISEDSDPFNIRSSGSKFSDSLKEDARKDLKFVSDVEKEMETLVEAVNKGKNSKKS
HSFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSVCPPTTLTGVLEREM
QARPPPPIPHHRHQSDKNPGSSNLQKITKEPIIDYFDVQD
Number of residues1120
Molecular Weight124393.645
Theoretical pINot Available
GO Classification
Functions
  • zinc ion binding
  • ligase activity
  • transcription factor activity, sequence-specific DNA binding
  • poly(A) RNA binding
  • RNA polymerase II regulatory region sequence-specific DNA binding
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
Processes
  • inflammatory response
  • transcription from RNA polymerase II promoter
  • negative regulation of transcription from RNA polymerase II promoter
  • viral process
  • negative regulation of MHC class II biosynthetic process
Components
  • cytoplasm
  • nucleus
  • plasma membrane
  • nucleolus
General FunctionZinc ion binding
Specific FunctionBinds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Isoform 3 binds to the X-box motif of TERT promoter and represses its expression. Together with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDQ12986
UniProtKB Entry NameNFX1_HUMAN
Cellular LocationNucleus
Gene sequence
>lcl|BSEQ0020865|Transcriptional repressor NF-X1 (NFX1)
ATGGCGGAGGCGCCTCCTGTCTCAGGTACTTTTAAATTCAATACAGATGCTGCTGAATTC
ATTCCTCAGGAGAAAAAAAATTCTGGTCTAAATTGTGGGACTCAAAGGAGACTAGACTCT
AATAGGATTGGTAGAAGAAATTACAGTTCACCACCTCCCTGTCACCTTTCCAGGCAGGTC
CCTTATGATGAAATCTCTGCTGTTCATCAGCATAGTTATCATCCGTCAGGAAGCAAACCT
AAGAGTCAGCAGACGTCTTTCCAGTCCTCTCCTTGTAATAAATCGCCCAAGAGCCATGGC
CTTCAGAATCAACCTTGGCAGAAATTGAGGAATGAGAAGCACCATATCAGAGTCAAGAAA
GCACAGAGTCTTGCTGAGCAGACCTCAGATACAGCTGGATTAGAGAGCTCGACCAGATCA
GAGAGTGGGACAGACCTCAGAGAGCATAGTCCTTCTGAGAGTGAGAAGGAAGTTGTGGGT
GCAGATCCCAGGGGAGCAAAACCCAAAAAAGCAACACAGTTTGTATACAGCTATGGTAGA
GGACCAAAAGTCAAGGGGAAACTCAAATGTGAATGGAGTAACCGAACAACTCCAAAACCG
GAGGATGCTGGACCCGAAAGTACCAAACCTGTGGGGGTTTTCCACCCTGACTCTTCAGAG
GCATCCTCTAGAAAAGGAGTATTGGATGGGTATGGAGCCAGACGAAATGAGCAGAGAAGA
TACCCACAGAAAAGGCCTCCCTGGGAAGTGGAGGGGGCCAGGCCACGACCAGGCAGAAAT
CCACCAAAACAGGAGGGCCACCGACATACAAACGCAGGACACAGAAACAACATGGGCCCC
ATTCCAAAGGATGACCTCAATGAAAGACCAGCAAAATCTACCTGTGACAGTGAGAACTTG
GCAGTCATCAACAAGTCTTCCAGGAGGGTTGACCAAGAGAAATGCACTGTACGGAGGCAG
GATCCTCAAGTAGTATCTCCTTTCTCCCGAGGCAAACAGAACCATGTGCTAAAGAATGTG
GAAACGCACACAGGTTCTCTAATTGAACAACTAACAACAGAAAAATACGAGTGCATGGTG
TGCTGTGAATTGGTTCGTGTCACGGCCCCAGTGTGGAGTTGTCAGAGCTGTTACCATGTG
TTTCATTTGAACTGCATAAAGAAATGGGCAAGGTCTCCAGCATCTCAAGCAGATGGCCAG
AGTGGTTGGAGGTGCCCTGCCTGTCAGAATGTTTCTGCACATGTTCCTAATACCTACACT
TGTTTCTGTGGCAAGGTAAAGAATCCTGAGTGGAGCAGAAATGAAATTCCACATAGCTGT
GGTGAGGTTTGTAGAAAGAAACAGCCTGGCCAGGACTGCCCACATTCCTGTAACCTTCTC
TGCCATCCAGGACCCTGCCCACCCTGCCCTGCCTTTATGACAAAAACATGTGAATGTGGA
CGAACCAGGCACACAGTTCGCTGTGGTCAGGCTGTCTCAGTCCACTGTTCTAACCCATGT
GAGAATATTTTGAACTGTGGTCAGCACCAGTGTGCTGAGCTGTGCCATGGGGGTCAGTGC
CAGCCTTGCCAGATCATTTTGAACCAGGTATGCTATTGCGGCAGCACCTCCCGAGATGTG
TTATGTGGAACCGATGTAGGAAAGTCTGATGGATTTGGGGATTTCAGCTGTTTAAAGATA
TGTGGCAAGGACTTGAAATGCGGTAACCATACATGTTCGCAAGTGTGCCACCCTCAGCCC
TGCCAGCAATGCCCACGGCTCCCCCAGCTGGTGCGCTGTTGCCCCTGTGGCCAAACTCCT
CTCAGCCAATTGCTAGAACTTGGAAGTAGTAGTCGGAAAACATGCATGGACCCTGTGCCT
TCATGTGGAAAAGTGTGCGGCAAGCCTCTGCCTTGTGGTTCCTTAGATTTCATTCATACC
TGTGAAAAGCTCTGCCATGAAGGAGACTGTGGACCATGCTCTCGCACATCAGTTATTTCC
TGCAGATGCTCTTTCAGAACAAAGGAGCTTCCATGTACCAGTCTCAAAAGTGAAGATGCT
ACATTTATGTGTGACAAGCGGTGTAACAAGAAACGGTTGTGTGGACGGCATAAATGTAAT
GAGATATGCTGTGTGGATAAGGAGCACAAGTGTCCTTTGATTTGTGGGAGGAAACTCCGT
TGTGGCCTTCATAGGTGTGAAGAACCTTGTCATCGTGGAAACTGCCAGACATGCTGGCAA
GCCAGTTTTGATGAATTAACCTGCCATTGTGGTGCATCAGTGATTTACCCTCCAGTTCCC
TGTGGTACTAGGCCCCCTGAATGTACCCAAACCTGCGCTAGAGTCCATGAGTGTGACCAT
CCAGTATATCATTCTTGTCATAGTGAGGAGAAGTGTCCCCCTTGCACTTTCCTAACTCAG
AAGTGGTGCATGGGCAAGCATGAGTTTCGGAGCAACATCCCCTGTCACCTGGTTGATATC
TCTTGCGGATTACCCTGCAGTGCCACGCTACCATGTGGGATGCACAAATGTCAGAGACTC
TGTCACAAAGGGGAGTGTCTTGTGGATGAGCCCTGCAAGCAGCCCTGCACCACCCCCAGA
GCTGACTGTGGTCACCCGTGTATGGCACCCTGCCATACCAGCTCACCCTGCCCTGTGACT
GCTTGTAAAGCTAAGGTAGAGCTACAGTGTGAATGTGGACGAAGAAAAGAGATGGTGATT
TGCTCTGAAGCATCTAGTACTTATCAAAGAATAGCTGCAATCTCCATGGCCTCTAAGATA
ACAGACATGCAGCTTGGAGGTTCAGTGGAGATCAGCAAGTTAATTACCAAAAAGGAAGTT
CATCAAGCCAGGCTGGAGTGTGATGAGGAGTGTTCAGCCTTGGAAAGGAAAAAGAGATTA
GCAGAGGCATTTCATATCAGTGAGGATTCTGATCCTTTCAATATACGTTCTTCAGGGTCA
AAATTCAGTGATAGTTTGAAAGAAGATGCCAGGAAGGACTTAAAGTTTGTCAGTGACGTT
GAGAAGGAAATGGAAACCCTCGTGGAGGCCGTGAATAAGGGAAAGAATAGTAAGAAAAGC
CACAGCTTCCCTCCCATGAACAGAGACCACCGCCGGATCATCCATGACTTGGCCCAAGTT
TATGGCCTGGAGAGCGTGAGCTATGACAGTGAACCGAAGCGCAATGTGGTGGTCACTGCC
ATCAGGGGGAAGTCCGTTTGTCCTCCTACCACGCTGACAGGTGTGCTTGAAAGGGAAATG
CAGGCACGGCCTCCACCACCGATTCCTCATCACAGACATCAGTCAGACAAGAATCCTGGG
AGCAGTAATTTACAGAAAATAACCAAGGAGCCAATAATTGACTATTTTGACGTCCAGGAC
TAA
GenBank Gene IDNot Available
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:7803
Chromosome Location9
LocusNot Available
References
  1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. 14702039
  2. Humphray SJ, Oliver K, Hunt AR, Plumb RW, Loveland JE, Howe KL, Andrews TD, Searle S, Hunt SE, Scott CE, Jones MC, Ainscough R, Almeida JP, Ambrose KD, Ashwell RI, Babbage AK, Babbage S, Bagguley CL, Bailey J, Banerjee R, Barker DJ, Barlow KF, Bates K, Beasley H, Beasley O, Bird CP, Bray-Allen S, Brown AJ, Brown JY, Burford D, Burrill W, Burton J, Carder C, Carter NP, Chapman JC, Chen Y, Clarke G, Clark SY, Clee CM, Clegg S, Collier RE, Corby N, Crosier M, Cummings AT, Davies J, Dhami P, Dunn M, Dutta I, Dyer LW, Earthrowl ME, Faulkner L, Fleming CJ, Frankish A, Frankland JA, French L, Fricker DG, Garner P, Garnett J, Ghori J, Gilbert JG, Glison C, Grafham DV, Gribble S, Griffiths C, Griffiths-Jones S, Grocock R, Guy J, Hall RE, Hammond S, Harley JL, Harrison ES, Hart EA, Heath PD, Henderson CD, Hopkins BL, Howard PJ, Howden PJ, Huckle E, Johnson C, Johnson D, Joy AA, Kay M, Keenan S, Kershaw JK, Kimberley AM, King A, Knights A, Laird GK, Langford C, Lawlor S, Leongamornlert DA, Leversha M, Lloyd C, Lloyd DM, Lovell J, Martin S, Mashreghi-Mohammadi M, Matthews L, McLaren S, McLay KE, McMurray A, Milne S, Nickerson T, Nisbett J, Nordsiek G, Pearce AV, Peck AI, Porter KM, Pandian R, Pelan S, Phillimore B, Povey S, Ramsey Y, Rand V, Scharfe M, Sehra HK, Shownkeen R, Sims SK, Skuce CD, Smith M, Steward CA, Swarbreck D, Sycamore N, Tester J, Thorpe A, Tracey A, Tromans A, Thomas DW, Wall M, Wallis JM, West AP, Whitehead SL, Willey DL, Williams SA, Wilming L, Wray PW, Young L, Ashurst JL, Coulson A, Blocker H, Durbin R, Sulston JE, Hubbard T, Jackson MJ, Bentley DR, Beck S, Rogers J, Dunham I: DNA sequence and analysis of human chromosome 9. Nature. 2004 May 27;429(6990):369-74. 15164053
  3. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. 15489334
  4. Song Z, Krishna S, Thanos D, Strominger JL, Ono SJ: A novel cysteine-rich sequence-specific DNA-binding protein interacts with the conserved X-box motif of the human major histocompatibility complex class II genes via a repeated Cys-His domain and functions as a transcriptional repressor. J Exp Med. 1994 Nov 1;180(5):1763-74. 7964459
  5. Lorick KL, Jensen JP, Fang S, Ong AM, Hatakeyama S, Weissman AM: RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11364-9. 10500182
  6. Gewin L, Myers H, Kiyono T, Galloway DA: Identification of a novel telomerase repressor that interacts with the human papillomavirus type-16 E6/E6-AP complex. Genes Dev. 2004 Sep 15;18(18):2269-82. 15371341
  7. Katzenellenbogen RA, Egelkrout EM, Vliet-Gregg P, Gewin LC, Gafken PR, Galloway DA: NFX1-123 and poly(A) binding proteins synergistically augment activation of telomerase in human papillomavirus type 16 E6-expressing cells. J Virol. 2007 Apr;81(8):3786-96. Epub 2007 Jan 31. 17267499
  8. Cantin GT, Yi W, Lu B, Park SK, Xu T, Lee JD, Yates JR 3rd: Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. J Proteome Res. 2008 Mar;7(3):1346-51. doi: 10.1021/pr0705441. Epub 2008 Jan 26. 18220336
  9. Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. 18669648
  10. Gauci S, Helbig AO, Slijper M, Krijgsveld J, Heck AJ, Mohammed S: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem. 2009 Jun 1;81(11):4493-501. doi: 10.1021/ac9004309. 19413330
  11. Oppermann FS, Gnad F, Olsen JV, Hornberger R, Greff Z, Keri G, Mann M, Daub H: Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics. 2009 Jul;8(7):1751-64. doi: 10.1074/mcp.M800588-MCP200. Epub 2009 Apr 15. 19369195
  12. Olsen JV, Vermeulen M, Santamaria A, Kumar C, Miller ML, Jensen LJ, Gnad F, Cox J, Jensen TS, Nigg EA, Brunak S, Mann M: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal. 2010 Jan 12;3(104):ra3. doi: 10.1126/scisignal.2000475. 20068231