T3DB: DNA-dependent protein kinase catalytic subunit

Name	DNA-dependent protein kinase catalytic subunit
Synonyms	2.7.11.1 DNA-PK catalytic subunit DNPK1 HYRC HYRC1 p460
Gene Name	PRKDC
Organism	Human
Amino acid sequence	>lcl\|BSEQ0004685\|DNA-dependent protein kinase catalytic subunit MAGSGAGVRCSLLRLQETLSAADRCGAALAGHQLIRGLGQECVLSSSPAVLALQTSLVFS RDFGLLVFVRKSLNSIEFRECREEILKFLCIFLEKMGQKIAPYSVEIKNTCTSVYTKDRA AKCKIPALDLLIKLLQTFRSSRLMDEFKIGELFSKFYGELALKKKIPDTVLEKVYELLGL LGEVHPSEMINNAENLFRAFLGELKTQMTSAVREPKLPVLAGCLKGLSSLLCNFTKSMEE DPQTSREIFNFVLKAIRPQIDLKRYAVPSAGLRLFALHASQFSTCLLDNYVSLFEVLLKW CAHTNVELKKAALSALESFLKQVSNMVAKNAEMHKNKLQYFMEQFYGIIRNVDSNNKELS IAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQMFLTQTDTGDDRVYQMPSFLQSVASV LLYLDTVPEVYTPVLEHLVVMQIDSFPQYSPKMQLVCCRAIVKVFLALAAKGPVLRNCIS TVVHQGLIRICSKPVVLPKGPESESEDHRASGEVRTGKWKVPTYKDYVDLFRHLLSSDQM MDSILADEAFFSVNSSSESLNHLLYDEFVKSVLKIVEKLDLTLEIQTVGEQENGDEAPGV WMIPTSDPAANLHPAKPKDFSAFINLVEFCREILPEKQAEFFEPWVYSFSYELILQSTRL PLISGFYKLLSITVRNAKKIKYFEGVSPKSLKHSPEDPEKYSCFALFVKFGKEVAVKMKQ YKDELLASCLTFLLSLPHNIIELDVRAYVPALQMAFKLGLSYTPLAEVGLNALEEWSIYI DRHVMQPYYKDILPCLDGYLKTSALSDETKNNWEVSALSRAAQKGFNKVVLKHLKKTKNL SSNEAISLEEIRIRVVQMLGSLGGQINKNLLTVTSSDEMMKSYVAWDREKRLSFAVPFRE MKPVIFLDVFLPRVTELALTASDRQTKVAACELLHSMVMFMLGKATQMPEGGQGAPPMYQ LYKRTFPVLLRLACDVDQVTRQLYEPLVMQLIHWFTNNKKFESQDTVALLEAILDGIVDP VDSTLRDFCGRCIREFLKWSIKQITPQQQEKSPVNTKSLFKRLYSLALHPNAFKRLGASL AFNNIYREFREEESLVEQFVFEALVIYMESLALAHADEKSLGTIQQCCDAIDHLCRIIEK KHVSLNKAKKRRLPRGFPPSASLCLLDLVKWLLAHCGRPQTECRHKSIELFYKFVPLLPG NRSPNLWLKDVLKEEGVSFLINTFEGGGCGQPSGILAQPTLLYLRGPFSLQATLCWLDLL LAALECYNTFIGERTVGALQVLGTEAQSSLLKAVAFFLESIAMHDIIAAEKCFGTGAAGN RTSPQEGERYNYSKCTVVVRIMEFTTTLLNTSPEGWKLLKKDLCNTHLMRVLVQTLCEPA SIGFNIGDVQVMAHLPDVCVNLMKALKMSPYKDILETHLREKITAQSIEELCAVNLYGPD AQVDRSRLAAVVSACKQLHRAGLLHNILPSQSTDLHHSVGTELLSLVYKGIAPGDERQCL PSLDLSCKQLASGLLELAFAFGGLCERLVSLLLNPAVLSTASLGSSQGSVIHFSHGEYFY SLFSETINTELLKNLDLAVLELMQSSVDNTKMVSAVLNGMLDQSFRERANQKHQGLKLAT TILQHWKKCDSWWAKDSPLETKMAVLALLAKILQIDSSVSFNTSHGSFPEVFTTYISLLA DTKLDLHLKGQAVTLLPFFTSLTGGSLEELRRVLEQLIVAHFPMQSREFPPGTPRFNNYV DCMKKFLDALELSQSPMLLELMTEVLCREQQHVMEELFQSSFRRIARRGSCVTQVGLLES VYEMFRKDDPRLSFTRQSFVDRSLLTLLWHCSLDALREFFSTIVVDAIDVLKSRFTKLNE STFDTQITKKMGYYKILDVMYSRLPKDDVHAKESKINQVFHGSCITEGNELTKTLIKLCY DAFTENMAGENQLLERRRLYHCAAYNCAISVICCVFNELKFYQGFLFSEKPEKNLLIFEN LIDLKRRYNFPVEVEVPMERKKKYIEIRKEAREAANGDSDGPSYMSSLSYLADSTLSEEM SQFDFSTGVQSYSYSSQDPRPATGRFRRREQRDPTVHDDVLELEMDELNRHECMAPLTAL VKHMHRSLGPPQGEEDSVPRDLPSWMKFLHGKLGNPIVPLNIRLFLAKLVINTEEVFRPY AKHWLSPLLQLAASENNGGEGIHYMVVEIVATILSWTGLATPTGVPKDEVLANRLLNFLM KHVFHPKRAVFRHNLEIIKTLVECWKDCLSIPYRLIFEKFSGKDPNSKDNSVGIQLLGIV MANDLPPYDPQCGIQSSEYFQALVNNMSFVRYKEVYAAAAEVLGLILRYVMERKNILEES LCELVAKQLKQHQNTMEDKFIVCLNKVTKSFPPLADRFMNAVFFLLPKFHGVLKTLCLEV VLCRVEGMTELYFQLKSKDFVQVMRHRDDERQKVCLDIIYKMMPKLKPVELRELLNPVVE FVSHPSTTCREQMYNILMWIHDNYRDPESETDNDSQEIFKLAKDVLIQGLIDENPGLQLI IRNFWSHETRLPSNTLDRLLALNSLYSPKIEVHFLSLATNFLLEMTSMSPDYPNPMFEHP LSECEFQEYTIDSDWRFRSTVLTPMFVETQASQGTLQTRTQEGSLSARWPVAGQIRATQQ QHDFTLTQTADGRSSFDWLTGSSTDPLVDHTSPSSDSLLFAHKRSERLQRAPLKSVGPDF GKKRLGLPGDEVDNKVKGAAGRTDLLRLRRRFMRDQEKLSLMYARKGVAEQKREKEIKSE LKMKQDAQVVLYRSYRHGDLPDIQIKHSSLITPLQAVAQRDPIIAKQLFSSLFSGILKEM DKFKTLSEKNNITQKLLQDFNRFLNTTFSFFPPFVSCIQDISCQHAALLSLDPAAVSAGC LASLQQPVGIRLLEEALLRLLPAELPAKRVRGKARLPPDVLRWVELAKLYRSIGEYDVLR GIFTSEIGTKQITQSALLAEARSDYSEAAKQYDEALNKQDWVDGEPTEAEKDFWELASLD CYNHLAEWKSLEYCSTASIDSENPPDLNKIWSEPFYQETYLPYMIRSKLKLLLQGEADQS LLTFIDKAMHGELQKAILELHYSQELSLLYLLQDDVDRAKYYIQNGIQSFMQNYSSIDVL LHQSRLTKLQSVQALTEIQEFISFISKQGNLSSQVPLKRLLNTWTNRYPDAKMDPMNIWD DIITNRCFFLSKIEEKLTPLPEDNSMNVDQDGDPSDRMEVQEQEEDISSLIRSCKFSMKM KMIDSARKQNNFSLAMKLLKELHKESKTRDDWLVSWVQSYCRLSHCRSRSQGCSEQVLTV LKTVSLLDENNVSSYLSKNILAFRDQNILLGTTYRIIANALSSEPACLAEIEEDKARRIL ELSGSSSEDSEKVIAGLYQRAFQHLSEAVQAAEEEAQPPSWSCGPAAGVIDAYMTLADFC DQQLRKEEENASVIDSAELQAYPALVVEKMLKALKLNSNEARLKFPRLLQIIERYPEETL SLMTKEISSVPCWQFISWISHMVALLDKDQAVAVQHSVEEITDNYPQAIVYPFIISSESY SFKDTSTGHKNKEFVARIKSKLDQGGVIQDFINALDQLSNPELLFKDWSNDVRAELAKTP VNKKNIEKMYERMYAALGDPKAPGLGAFRRKFIQTFGKEFDKHFGKGGSKLLRMKLSDFN DITNMLLLKMNKDSKPPGNLKECSPWMSDFKVEFLRNELEIPGQYDGRGKPLPEYHVRIA GFDERVTVMASLRRPKRIIIRGHDEREHPFLVKGGEDLRQDQRVEQLFQVMNGILAQDSA CSQRALQLRTYSVVPMTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAYLSDPRAPPCEYK DWLTKMSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPADLLKRAFVRMSTSPEAFLAL RSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHAFGSATQFLPVPELMP FRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDPGLLTNTMDVFVKEPSFDWKNFEQKM LKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAV ARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILGRTWEGWEPWM
Number of residues	4128
Molecular Weight	469084.155
Theoretical pI	7.12
GO Classification	Functions poly(A) RNA binding DNA-dependent protein kinase activity double-stranded DNA binding ATP binding protein kinase activity protein serine/threonine kinase activity transcription factor binding Processes peptidyl-serine phosphorylation regulation of circadian rhythm cellular protein modification process spleen development response to gamma radiation double-strand break repair negative regulation of immunoglobulin production negative regulation of protein phosphorylation double-strand break repair via homologous recombination innate immune response somitogenesis intrinsic apoptotic signaling pathway in response to DNA damage positive regulation of immune system process positive regulation of type I interferon production positive regulation of apoptotic process negative regulation of cellular senescence regulation of smooth muscle cell proliferation heart development B cell lineage commitment T cell differentiation in thymus negative regulation of apoptotic process immunoglobulin V(D)J recombination thymus development positive regulation of transcription from RNA polymerase II promoter negative regulation of response to gamma radiation pro-B cell differentiation protein destabilization response to activity signal transduction involved in mitotic G1 DNA damage checkpoint DNA repair ectopic germ cell programmed cell death cellular response to insulin stimulus T cell lineage commitment brain development T cell receptor V(D)J recombination rhythmic process positive regulation of developmental growth double-strand break repair via nonhomologous end joining telomere maintenance positive regulation of fibroblast proliferation Components nucleolus DNA-dependent protein kinase-DNA ligase 4 complex nucleoplasm nonhomologous end joining complex transcription factor complex membrane nuclear chromosome, telomeric region cytosol
General Function	Transcription factor binding
Specific Function	Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation.
Pfam Domain Function	PI3_PI4_kinase (PF00454 ) FAT (PF02259 ) FATC (PF02260 ) NUC194 (PF08163 )
Transmembrane Regions	Not Available
GenBank Protein ID	Not Available
UniProtKB ID	P78527
UniProtKB Entry Name	PRKDC_HUMAN
Cellular Location	Nucleus
Gene sequence	>lcl\|BSEQ0016774\|DNA-dependent protein kinase catalytic subunit (PRKDC) ATGGCGGGCTCCGGAGCCGGTGTGCGTTGCTCCCTGCTGCGGCTGCAGGAGACCTTGTCC GCTGCGGACCGCTGCGGTGCTGCCCTGGCCGGTCATCAACTGATCCGCGGCCTGGGGCAG GAATGCGTCCTGAGCAGCAGCCCCGCGGTGCTGGCATTACAGACATCTTTAGTTTTTTCC AGAGATTTCGGTTTGCTTGTATTTGTCCGGAAGTCACTCAACAGTATTGAATTTCGTGAA TGTAGAGAAGAAATCCTAAAGTTTTTATGTATTTTCTTAGAAAAAATGGGCCAGAAGATC GCACCTTACTCTGTTGAAATTAAGAACACTTGTACCAGTGTTTATACAAAAGATAGAGCT GCTAAATGTAAAATTCCAGCCCTGGACCTTCTTATTAAGTTACTTCAGACTTTTAGAAGT TCTAGACTCATGGATGAATTTAAAATTGGAGAATTATTTAGTAAATTCTATGGAGAACTT GCATTGAAAAAAAAAATACCAGATACAGTTTTAGAAAAAGTATATGAGCTCCTAGGATTA TTGGGTGAAGTTCATCCTAGTGAGATGATAAATAATGCAGAAAACCTGTTCCGCGCTTTT CTGGGTGAACTTAAGACCCAGATGACATCAGCAGTAAGAGAGCCCAAACTACCTGTTCTG GCAGGATGTCTGAAGGGGTTGTCCTCACTTCTGTGCAACTTCACTAAGTCCATGGAAGAA GATCCCCAGACTTCAAGGGAGATTTTTAATTTTGTACTAAAGGCAATTCGTCCTCAGATT GATCTGAAGAGATATGCTGTGCCCTCAGCTGGCTTGCGCCTATTTGCCCTGCATGCATCT CAGTTTAGCACCTGCCTTCTGGACAACTACGTGTCTCTATTTGAAGTCTTGTTAAAGTGG TGTGCCCACACAAATGTAGAATTGAAAAAAGCTGCACTTTCAGCCCTGGAATCCTTTCTG AAACAGGTTTCTAATATGGTGGCGAAAAATGCAGAAATGCATAAAAATAAACTGCAGTAC TTTATGGAGCAGTTTTATGGAATCATCAGAAATGTGGATTCGAACAACAAGGAGTTATCT ATTGCTATCCGTGGATATGGACTTTTTGCAGGACCGTGCAAGGTTATAAACGCAAAAGAT GTTGACTTCATGTACGTTGAGCTCATTCAGCGCTGCAAGCAGATGTTCCTCACCCAGACA GACACTGGTGACGACCGTGTTTATCAGATGCCAAGCTTCCTCCAGTCTGTTGCAAGCGTC TTGCTGTACCTTGACACAGTTCCTGAGGTGTATACTCCAGTTCTGGAGCACCTCGTGGTG ATGCAGATAGACAGTTTCCCACAGTACAGTCCAAAAATGCAGCTGGTGTGTTGCAGAGCC ATAGTGAAGGTGTTCCTAGCTTTGGCAGCAAAAGGGCCAGTTCTCAGGAATTGCATTAGT ACTGTGGTGCATCAGGGTTTAATCAGAATATGTTCTAAACCAGTGGTCCTTCCAAAGGGC CCTGAGTCTGAATCTGAAGACCACCGTGCTTCAGGGGAAGTCAGAACTGGCAAATGGAAG GTGCCCACATACAAAGACTACGTGGATCTCTTCAGACATCTCCTGAGCTCTGACCAGATG ATGGATTCTATTTTAGCAGATGAAGCATTTTTCTCTGTGAATTCCTCCAGTGAAAGTCTG AATCATTTACTTTATGATGAATTTGTAAAATCCGTTTTGAAGATTGTTGAGAAATTGGAT CTTACACTTGAAATACAGACTGTTGGGGAACAAGAGAATGGAGATGAGGCGCCTGGTGTT TGGATGATCCCAACTTCAGATCCAGCGGCTAACTTGCATCCAGCTAAACCTAAAGATTTT TCGGCTTTCATTAACCTGGTGGAATTTTGCAGAGAGATTCTCCCTGAGAAACAAGCAGAA TTTTTTGAACCATGGGTGTACTCATTTTCATATGAATTAATTTTGCAATCTACAAGGTTG CCCCTCATCAGTGGTTTCTACAAATTGCTTTCTATTACAGTAAGAAATGCCAAGAAAATA AAATATTTCGAGGGAGTTAGTCCAAAGAGTCTGAAACACTCTCCTGAAGACCCAGAAAAG TATTCTTGCTTTGCTTTATTTGTGAAATTTGGCAAAGAGGTGGCAGTTAAAATGAAGCAG TACAAAGATGAACTTTTGGCCTCTTGTTTGACCTTTCTTCTGTCCTTGCCACACAACATC ATTGAACTCGATGTTAGAGCCTACGTTCCTGCACTGCAGATGGCTTTCAAACTGGGCCTG AGCTATACCCCCTTGGCAGAAGTAGGCCTGAATGCTCTAGAAGAATGGTCAATTTATATT GACAGACATGTAATGCAGCCTTATTACAAAGACATTCTCCCCTGCCTGGATGGATACCTG AAGACTTCAGCCTTGTCAGATGAGACCAAGAATAACTGGGAAGTGTCAGCTCTTTCTCGG GCTGCCCAGAAAGGATTTAATAAAGTGGTGTTAAAGCATCTGAAGAAGACAAAGAACCTT TCATCAAACGAAGCAATATCCTTAGAAGAAATAAGAATTAGAGTAGTACAAATGCTTGGA TCTCTAGGAGGACAAATAAACAAAAATCTTCTGACAGTCACGTCCTCAGATGAGATGATG AAGAGCTATGTGGCCTGGGACAGAGAGAAGCGGCTGAGCTTTGCAGTGCCCTTTAGAGAG ATGAAACCTGTCATTTTCCTGGATGTGTTCCTGCCTCGAGTCACAGAATTAGCGCTCACA GCCAGTGACAGACAAACTAAAGTTGCAGCCTGTGAACTTTTACATAGCATGGTTATGTTT ATGTTGGGCAAAGCCACGCAGATGCCAGAAGGGGGACAGGGAGCCCCACCCATGTACCAG CTCTATAAGCGGACGTTTCCTGTGCTGCTTCGACTTGCGTGTGATGTTGATCAGGTGACA AGGCAACTGTATGAGCCACTAGTTATGCAGCTGATTCACTGGTTCACTAACAACAAGAAA TTTGAAAGTCAGGATACTGTTGCCTTACTAGAAGCTATATTGGATGGAATTGTGGACCCT GTTGACAGTACTTTAAGAGATTTTTGTGGTCGGTGTATTCGAGAATTCCTTAAATGGTCC ATTAAGCAAATAACACCACAGCAGCAGGAGAAGAGTCCAGTAAACACCAAATCGCTTTTC AAGCGACTTTATAGCCTTGCGCTTCACCCCAATGCTTTCAAGAGGCTGGGAGCATCACTT GCCTTTAATAATATCTACAGGGAATTCAGGGAAGAAGAGTCTCTGGTGGAACAGTTTGTG TTTGAAGCCTTGGTGATATACATGGAGAGTCTGGCCTTAGCACATGCAGATGAGAAGTCC TTAGGTACAATTCAACAGTGTTGTGATGCCATTGATCACCTATGCCGCATCATTGAAAAG AAGCATGTTTCTTTAAATAAAGCAAAGAAACGACGTTTGCCGCGAGGATTTCCACCTTCC GCATCATTGTGTTTATTGGATCTGGTCAAGTGGCTTTTAGCTCATTGTGGGAGGCCCCAG ACAGAATGTCGACACAAATCCATTGAACTCTTTTATAAATTCGTTCCTTTATTGCCAGGC AACAGATCCCCTAATTTGTGGCTGAAAGATGTTCTCAAGGAAGAAGGTGTCTCTTTTCTC ATCAACACCTTTGAGGGGGGTGGCTGTGGCCAGCCCTCGGGCATCCTGGCCCAGCCCACC CTCTTGTACCTTCGGGGGCCATTCAGCCTGCAGGCCACGCTATGCTGGCTGGACCTGCTC CTGGCCGCGTTGGAGTGCTACAACACGTTCATTGGCGAGAGAACTGTAGGAGCGCTCCAG GTCCTAGGTACTGAAGCCCAGTCTTCACTTTTGAAAGCAGTGGCTTTCTTCTTAGAAAGC ATTGCCATGCATGACATTATAGCAGCAGAAAAGTGCTTTGGCACTGGGGCAGCAGGTAAC AGAACAAGCCCACAAGAGGGAGAAAGGTACAACTACAGCAAATGCACCGTTGTGGTCCGG ATTATGGAGTTTACCACGACTCTGCTAAACACCTCCCCGGAAGGATGGAAGCTCCTGAAG AAGGACTTGTGTAATACACACCTGATGAGAGTCCTGGTGCAGACGCTGTGTGAGCCCGCA AGCATAGGTTTCAACATCGGAGACGTCCAGGTTATGGCTCATCTTCCTGATGTTTGTGTG AATCTGATGAAAGCTCTAAAGATGTCCCCATACAAAGATATCCTAGAGACCCATCTGAGA GAGAAAATAACAGCACAGAGCATTGAGGAGCTTTGTGCCGTCAACTTGTATGGCCCTGAC GCGCAAGTGGACAGGAGCAGGCTGGCTGCTGTTGTGTCTGCCTGTAAACAGCTTCACAGA GCTGGGCTTCTGCATAATATATTACCGTCTCAGTCCACAGATTTGCATCATTCTGTTGGC ACAGAACTTCTTTCCCTGGTTTATAAAGGCATTGCCCCTGGAGATGAGAGACAGTGTCTG CCTTCTCTAGACCTCAGTTGTAAGCAGCTGGCCAGCGGACTTCTGGAGTTAGCCTTTGCT TTTGGAGGACTGTGTGAGCGCCTTGTGAGTCTTCTCCTGAACCCAGCGGTGCTGTCCACG GCGTCCTTGGGCAGCTCACAGGGCAGCGTCATCCACTTCTCCCATGGGGAGTATTTCTAT AGCTTGTTCTCAGAAACGATCAACACGGAATTATTGAAAAATCTGGATCTTGCTGTATTG GAGCTCATGCAGTCTTCAGTGGATAATACCAAAATGGTGAGTGCCGTTTTGAACGGCATG TTAGACCAGAGCTTCAGGGAGCGAGCAAACCAGAAACACCAAGGACTGAAACTTGCGACT ACAATTCTGCAACACTGGAAGAAGTGTGATTCATGGTGGGCCAAAGATTCCCCTCTCGAA ACTAAAATGGCAGTGCTGGCCTTACTGGCAAAAATTTTACAGATTGATTCATCTGTATCT TTTAATACAAGTCATGGTTCATTCCCTGAAGTCTTTACAACATATATTAGTCTACTTGCT GACACAAAGCTGGATCTACATTTAAAGGGCCAAGCTGTCACTCTTCTTCCATTCTTCACC AGCCTCACTGGAGGCAGTCTGGAGGAACTTAGACGTGTTCTGGAGCAGCTCATCGTTGCT CACTTCCCCATGCAGTCCAGGGAATTTCCTCCAGGAACTCCGCGGTTCAATAATTATGTG GACTGCATGAAAAAGTTTCTAGATGCATTGGAATTATCTCAAAGCCCTATGTTGTTGGAA TTGATGACAGAAGTTCTTTGTCGGGAACAGCAGCATGTCATGGAAGAATTATTTCAATCC AGTTTCAGGAGGATTGCCAGAAGGGGTTCATGTGTCACACAAGTAGGCCTTCTGGAAAGC GTGTATGAAATGTTCAGGAAGGATGACCCCCGCCTAAGTTTCACACGCCAGTCCTTTGTG GACCGCTCCCTCCTCACTCTGCTGTGGCACTGTAGCCTGGATGCTTTGAGAGAATTCTTC AGCACAATTGTGGTGGATGCCATTGATGTGTTGAAGTCCAGGTTTACAAAGCTAAATGAA TCTACCTTTGATACTCAAATCACCAAGAAGATGGGCTACTATAAGATTCTAGACGTGATG TATTCTCGCCTTCCCAAAGATGATGTTCATGCTAAGGAATCAAAAATTAATCAAGTTTTC CATGGCTCGTGTATTACAGAAGGAAATGAACTTACAAAGACATTGATTAAATTGTGCTAC GATGCATTTACAGAGAACATGGCAGGAGAGAATCAGCTGCTGGAGAGGAGAAGACTTTAC CATTGTGCAGCATACAACTGCGCCATATCTGTCATCTGCTGTGTCTTCAATGAGTTAAAA TTTTACCAAGGTTTTCTGTTTAGTGAAAAACCAGAAAAGAACTTGCTTATTTTTGAAAAT CTGATCGACCTGAAGCGCCGCTATAATTTTCCTGTAGAAGTTGAGGTTCCTATGGAAAGA AAGAAAAAGTACATTGAAATTAGGAAAGAAGCCAGAGAAGCAGCAAATGGGGATTCAGAT GGTCCTTCCTATATGTCTTCCCTGTCATATTTGGCAGACAGTACCCTGAGTGAGGAAATG AGTCAATTTGATTTCTCAACCGGAGTTCAGAGCTATTCATACAGCTCCCAAGACCCTAGA CCTGCCACTGGTCGTTTTCGGAGACGGGAGCAGCGGGACCCCACGGTGCATGATGATGTG CTGGAGCTGGAGATGGACGAGCTCAATCGGCATGAGTGCATGGCGCCCCTGACGGCCCTG GTCAAGCACATGCACAGAAGCCTGGGCCCGCCTCAAGGAGAAGAGGATTCAGTGCCAAGA GATCTTCCTTCTTGGATGAAATTCCTCCATGGCAAACTGGGAAATCCAATAGTACCATTA AATATCCGTCTCTTCTTAGCCAAGCTTGTTATTAATACAGAAGAGGTCTTTCGCCCTTAC GCGAAGCACTGGCTTAGCCCCTTGCTGCAGCTGGCTGCTTCTGAAAACAATGGAGGAGAA GGAATTCACTACATGGTGGTTGAGATAGTGGCCACTATTCTTTCATGGACAGGCTTGGCC ACTCCAACAGGGGTCCCTAAAGATGAAGTGTTAGCAAATCGATTGCTTAATTTCCTAATG AAACATGTCTTTCATCCAAAAAGAGCTGTGTTTAGACACAACCTTGAAATTATAAAGACC CTTGTCGAGTGCTGGAAGGATTGTTTATCCATCCCTTATAGGTTAATATTTGAAAAGTTT TCCGGTAAAGATCCTAATTCTAAAGACAACTCAGTAGGGATTCAATTGCTAGGCATCGTG ATGGCCAATGACCTGCCTCCCTATGACCCACAGTGTGGCATCCAGAGTAGCGAATACTTC CAGGCTTTGGTGAATAATATGTCCTTTGTAAGATATAAAGAAGTGTATGCCGCTGCAGCA GAAGTTCTAGGACTTATACTTCGATATGTTATGGAGAGAAAAAACATACTGGAGGAGTCT CTGTGTGAACTGGTTGCGAAACAATTGAAGCAACATCAGAATACTATGGAGGACAAGTTT ATTGTGTGCTTGAACAAAGTGACCAAGAGCTTCCCTCCTCTTGCAGACAGGTTCATGAAT GCTGTGTTCTTTCTGCTGCCAAAATTTCATGGAGTGTTGAAAACACTCTGTCTGGAGGTG GTACTTTGTCGTGTGGAGGGAATGACAGAGCTGTACTTCCAGTTAAAGAGCAAGGACTTC GTTCAAGTCATGAGACATAGAGATGATGAAAGACAAAAAGTATGTTTGGACATAATTTAT AAGATGATGCCAAAGTTAAAACCAGTAGAACTCCGAGAACTTCTGAACCCCGTTGTGGAA TTCGTTTCCCATCCTTCTACAACATGTAGGGAACAAATGTATAATATTCTCATGTGGATT CATGATAATTACAGAGATCCAGAAAGTGAGACAGATAATGACTCCCAGGAAATATTTAAG TTGGCAAAAGATGTGCTGATTCAAGGATTGATCGATGAGAACCCTGGACTTCAATTAATT ATTCGAAATTTCTGGAGCCATGAAACTAGGTTACCTTCAAATACCTTGGACCGGTTGCTG GCACTAAATTCCTTATATTCTCCTAAGATAGAAGTGCACTTTTTAAGTTTAGCAACAAAT TTTCTGCTCGAAATGACCAGCATGAGCCCAGATTATCCAAACCCCATGTTCGAGCATCCT CTGTCAGAATGCGAATTTCAGGAATATACCATTGATTCTGATTGGCGTTTCCGAAGTACT GTTCTCACTCCGATGTTTGTGGAGACCCAGGCCTCCCAGGGCACTCTCCAGACCCGTACC CAGGAAGGGTCCCTCTCAGCTCGCTGGCCAGTGGCAGGGCAGATAAGGGCCACCCAGCAG CAGCATGACTTCACACTGACACAGACTGCAGATGGAAGAAGCTCATTTGATTGGCTGACC GGGAGCAGCACTGACCCGCTGGTCGACCACACCAGTCCCTCATCTGACTCCTTGCTGTTT GCCCACAAGAGGAGTGAAAGGTTACAGAGAGCACCCTTGAAGTCAGTGGGGCCTGATTTT GGGAAAAAAAGGCTGGGCCTTCCAGGGGACGAGGTGGATAACAAAGTGAAAGGTGCGGCC GGCCGGACGGACCTACTACGACTGCGCAGACGGTTTATGAGGGACCAGGAGAAGCTCAGT TTGATGTATGCCAGAAAAGGCGTTGCTGAGCAAAAACGAGAGAAGGAAATCAAGAGTGAG TTAAAAATGAAGCAGGATGCCCAGGTCGTTCTGTACAGAAGCTACCGGCACGGAGACCTT CCTGACATTCAGATCAAGCACAGCAGCCTCATCACCCCGTTACAGGCCGTGGCCCAGAGG GACCCAATAATTGCAAAACAGCTCTTTAGCAGCTTGTTTTCTGGAATTTTGAAAGAGATG GATAAATTTAAGACACTGTCTGAAAAAAACAACATCACTCAAAAGTTGCTTCAAGACTTC AATCGTTTTCTTAATACCACCTTCTCTTTCTTTCCACCCTTTGTCTCTTGTATTCAGGAC ATTAGCTGTCAGCACGCAGCCCTGCTGAGCCTCGACCCAGCGGCTGTTAGCGCTGGTTGC CTGGCCAGCCTACAGCAGCCCGTGGGCATCCGCCTGCTAGAGGAGGCTCTGCTCCGCCTG CTGCCTGCTGAGCTGCCTGCCAAGCGAGTCCGTGGGAAGGCCCGCCTCCCTCCTGATGTC CTCAGATGGGTGGAGCTTGCTAAGCTGTATAGATCAATTGGAGAATACGACGTCCTCCGT GGGATTTTTACCAGTGAGATAGGAACAAAGCAAATCACTCAGAGTGCATTATTAGCAGAA GCCAGAAGTGATTATTCTGAAGCTGCTAAGCAGTATGATGAGGCTCTCAATAAACAAGAC TGGGTAGATGGTGAGCCCACAGAAGCCGAGAAGGATTTTTGGGAACTTGCATCCCTTGAC TGTTACAACCACCTTGCTGAGTGGAAATCACTTGAATACTGTTCTACAGCCAGTATAGAC AGTGAGAACCCCCCAGACCTAAATAAAATCTGGAGTGAACCATTTTATCAGGAAACATAT CTACCTTACATGATCCGCAGCAAGCTGAAGCTGCTGCTCCAGGGAGAGGCTGACCAGTCC CTGCTGACATTTATTGACAAAGCTATGCACGGGGAGCTCCAGAAGGCGATTCTAGAGCTT CATTACAGTCAAGAGCTGAGTCTGCTTTACCTCCTGCAAGATGATGTTGACAGAGCCAAA TATTACATTCAAAATGGCATTCAGAGTTTTATGCAGAATTATTCTAGTATTGATGTCCTC TTACACCAAAGTAGACTCACCAAATTGCAGTCTGTACAGGCTTTAACAGAAATTCAGGAG TTCATCAGCTTTATAAGCAAACAAGGCAATTTATCATCTCAAGTTCCCCTTAAGAGACTT CTGAACACCTGGACAAACAGATATCCAGATGCTAAAATGGACCCAATGAACATCTGGGAT GACATCATCACAAATCGATGTTTCTTTCTCAGCAAAATAGAGGAGAAGCTTACCCCTCTT CCAGAAGATAATAGTATGAATGTGGATCAAGATGGAGACCCCAGTGACAGGATGGAAGTG CAAGAGCAGGAAGAAGATATCAGCTCCCTGATCAGGAGTTGCAAGTTTTCCATGAAAATG AAGATGATAGACAGTGCCCGGAAGCAGAACAATTTCTCACTTGCTATGAAACTACTGAAG GAGCTGCATAAAGAGTCAAAAACCAGAGACGATTGGCTGGTGAGCTGGGTGCAGAGCTAC TGCCGCCTGAGCCACTGCCGGAGCCGGTCCCAGGGCTGCTCTGAGCAGGTGCTCACTGTG CTGAAAACAGTCTCTTTGTTGGATGAGAACAACGTGTCAAGCTACTTAAGCAAAAATATT CTGGCTTTCCGTGACCAGAACATTCTCTTGGGTACAACTTACAGGATCATAGCGAATGCT CTCAGCAGTGAGCCAGCCTGCCTTGCTGAAATCGAGGAGGACAAGGCTAGAAGAATCTTA GAGCTTTCTGGATCCAGTTCAGAGGATTCAGAGAAGGTGATCGCGGGTCTGTACCAGAGA GCATTCCAGCACCTCTCTGAGGCTGTGCAGGCGGCTGAGGAGGAGGCCCAGCCTCCCTCC TGGAGCTGTGGGCCTGCAGCTGGGGTGATTGATGCTTACATGACGCTGGCAGATTTCTGT GACCAACAGCTGCGCAAGGAGGAAGAGAATGCATCAGTTATTGATTCTGCAGAACTGCAG GCGTATCCAGCACTTGTGGTGGAGAAAATGTTGAAAGCTTTAAAATTAAATTCCAATGAA GCCAGATTGAAGTTTCCTAGATTACTTCAGATTATAGAACGGTATCCAGAGGAGACTTTG AGCCTCATGACAAAAGAGATCTCTTCCGTTCCCTGCTGGCAGTTCATCAGCTGGATCAGC CACATGGTGGCCTTACTGGACAAAGACCAAGCCGTTGCTGTTCAGCACTCTGTGGAAGAA ATCACTGATAACTACCCGCAGGCTATTGTTTATCCCTTCATCATAAGCAGCGAAAGCTAT TCCTTCAAGGATACTTCTACTGGTCATAAGAATAAGGAGTTTGTGGCAAGGATTAAAAGT AAGTTGGATCAAGGAGGAGTGATTCAAGATTTTATTAATGCCTTAGATCAGCTCTCTAAT CCTGAACTGCTCTTTAAGGATTGGAGCAATGATGTAAGAGCTGAACTAGCAAAAACCCCT GTAAATAAAAAAAACATTGAAAAAATGTATGAAAGAATGTATGCAGCCTTGGGTGACCCA AAGGCTCCAGGCCTGGGGGCCTTTAGAAGGAAGTTTATTCAGACTTTTGGAAAAGAATTT GATAAACATTTTGGGAAAGGAGGTTCTAAACTACTGAGAATGAAGCTCAGTGACTTCAAC GACATTACCAACATGCTACTTTTAAAAATGAACAAAGACTCAAAGCCCCCTGGGAATCTG AAAGAATGTTCACCCTGGATGAGCGACTTCAAAGTGGAGTTCCTGAGAAATGAGCTGGAG ATTCCCGGTCAGTATGACGGTAGGGGAAAGCCATTGCCAGAGTACCACGTGCGAATCGCC GGGTTTGATGAGCGGGTGACAGTCATGGCGTCTCTGCGAAGGCCCAAGCGCATCATCATC CGTGGCCATGACGAGAGGGAACACCCTTTCCTGGTGAAGGGTGGCGAGGACCTGCGGCAG GACCAGCGCGTGGAGCAGCTCTTCCAGGTCATGAATGGGATCCTGGCCCAAGACTCCGCC TGCAGCCAGAGGGCCCTGCAGCTGAGGACCTATAGCGTTGTGCCCATGACCTCCAGTGAT CCCAGGGCACCGCCGTGTGAATATAAAGATTGGCTGACAAAAATGTCAGGAAAACATGAT GTTGGAGCTTACATGCTAATGTATAAGGGCGCTAATCGTACTGAAACAGTCACGTCTTTT AGAAAACGAGAAAGTAAAGTGCCTGCTGATCTCTTAAAGCGGGCCTTCGTGAGGATGAGT ACAAGCCCTGAGGCTTTCCTGGCGCTCCGCTCCCACTTCGCCAGCTCTCACGCTCTGATA TGCATCAGCCACTGGATCCTCGGGATTGGAGACAGACATCTGAACAACTTTATGGTGGCC ATGGAGACTGGCGGCGTGATCGGGATCGACTTTGGGCATGCGTTTGGATCCGCTACACAG TTTCTGCCAGTCCCTGAGTTGATGCCTTTTCGGCTAACTCGCCAGTTTATCAATCTGATG TTACCAATGAAAGAAACGGGCCTTATGTACAGCATCATGGTACACGCACTCCGGGCCTTC CGCTCAGACCCTGGCCTGCTCACCAACACCATGGATGTGTTTGTCAAGGAGCCCTCCTTT GATTGGAAAAATTTTGAACAGAAAATGCTGAAAAAAGGAGGGTCATGGATTCAAGAAATA AATGTTGCTGAAAAAAATTGGTACCCCCGACAGAAAATATGTTACGCTAAGAGAAAGTTA GCAGGTGCCAATCCAGCAGTCATTACTTGTGATGAGCTACTCCTGGGTCATGAGAAGGCC CCTGCCTTCAGAGACTATGTGGCTGTGGCACGAGGAAGCAAAGATCACAACATTCGTGCC CAAGAACCAGAGAGTGGGCTTTCAGAAGAGACTCAAGTGAAGTGCCTGATGGACCAGGCA ACAGACCCCAACATCCTTGGCAGAACCTGGGAAGGATGGGAGCCCTGGATGTGA
GenBank Gene ID	U47077
GeneCard ID	Not Available
GenAtlas ID	PRKDC
HGNC ID	HGNC:9413
Chromosome Location	8
Locus	8q11
References	Hartley KO, Gell D, Smith GC, Zhang H, Divecha N, Connelly MA, Admon A, Lees-Miller SP, Anderson CW, Jackson SP: DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product. Cell. 1995 Sep 8;82(5):849-56. 7671312 Anderson CW, Dunn JJ, Freimuth PI, Galloway AM, Allalunis-Turner MJ: Frameshift mutation in PRKDC, the gene for DNA-PKcs, in the DNA repair-defective, human, glioma-derived cell line M059J. Radiat Res. 2001 Jul;156(1):2-9. 11418067 Ladenburger EM, Fackelmayer FO, Hameister H, Knippers R: MCM4 and PRKDC, human genes encoding proteins MCM4 and DNA-PKcs, are close neighbours located on chromosome 8q12-->q13. Cytogenet Cell Genet. 1997;77(3-4):268-70. 9284934 Sipley JD, Menninger JC, Hartley KO, Ward DC, Jackson SP, Anderson CW: Gene for the catalytic subunit of the human DNA-activated protein kinase maps to the site of the XRCC7 gene on chromosome 8. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7515-9. 7638222 Poltoratsky VP, Shi X, York JD, Lieber MR, Carter TH: Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases. J Immunol. 1995 Nov 15;155(10):4529-33. 7594449 Lees-Miller SP, Anderson CW: The human double-stranded DNA-activated protein kinase phosphorylates the 90-kDa heat-shock protein, hsp90 alpha at two NH2-terminal threonine residues. J Biol Chem. 1989 Oct 15;264(29):17275-80. 2507541 Carter T, Vancurova I, Sun I, Lou W, DeLeon S: A DNA-activated protein kinase from HeLa cell nuclei. Mol Cell Biol. 1990 Dec;10(12):6460-71. 2247066 Iijima S, Teraoka H, Date T, Tsukada K: DNA-activated protein kinase in Raji Burkitt's lymphoma cells. Phosphorylation of c-Myc oncoprotein. Eur J Biochem. 1992 Jun 1;206(2):595-603. 1597196 Liu SH, Ma JT, Yueh AY, Lees-Miller SP, Anderson CW, Ng SY: The carboxyl-terminal transactivation domain of human serum response factor contains DNA-activated protein kinase phosphorylation sites. J Biol Chem. 1993 Oct 5;268(28):21147-54. 8407951 Bannister AJ, Gottlieb TM, Kouzarides T, Jackson SP: c-Jun is phosphorylated by the DNA-dependent protein kinase in vitro; definition of the minimal kinase recognition motif. Nucleic Acids Res. 1993 Mar 11;21(5):1289-95. 8464713 Teraoka H, Yumoto Y, Watanabe F, Tsukada K, Suwa A, Enari M, Nagata S: CPP32/Yama/apopain cleaves the catalytic component of DNA-dependent protein kinase in the holoenzyme. FEBS Lett. 1996 Sep 9;393(1):1-6. 8804412 Connelly MA, Zhang H, Kieleczawa J, Anderson CW: Alternate splice-site utilization in the gene for the catalytic subunit of the DNA-activated protein kinase, DNA-PKcs. Gene. 1996 Oct 10;175(1-2):271-3. 8917110 Shieh SY, Ikeda M, Taya Y, Prives C: DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2. Cell. 1997 Oct 31;91(3):325-34. 9363941 Wu X, Lieber MR: Interaction between DNA-dependent protein kinase and a novel protein, KIP. Mutat Res. 1997 Oct;385(1):13-20. 9372844 Jin S, Weaver DT: Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions. EMBO J. 1997 Nov 17;16(22):6874-85. 9362500 Niu H, Erdjument-Bromage H, Pan ZQ, Lee SH, Tempst P, Hurwitz J: Mapping of amino acid residues in the p34 subunit of human single-stranded DNA-binding protein phosphorylated by DNA-dependent protein kinase and Cdc2 kinase in vitro. J Biol Chem. 1997 May 9;272(19):12634-41. 9139719 Sarkaria JN, Tibbetts RS, Busby EC, Kennedy AP, Hill DE, Abraham RT: Inhibition of phosphoinositide 3-kinase related kinases by the radiosensitizing agent wortmannin. Cancer Res. 1998 Oct 1;58(19):4375-82. 9766667 Yavuzer U, Smith GC, Bliss T, Werner D, Jackson SP: DNA end-independent activation of DNA-PK mediated via association with the DNA-binding protein C1D. Genes Dev. 1998 Jul 15;12(14):2188-99. 9679063 Ting NS, Kao PN, Chan DW, Lintott LG, Lees-Miller SP: DNA-dependent protein kinase interacts with antigen receptor response element binding proteins NF90 and NF45. J Biol Chem. 1998 Jan 23;273(4):2136-45. 9442054 Hammarsten O, Chu G: DNA-dependent protein kinase: DNA binding and activation in the absence of Ku. Proc Natl Acad Sci U S A. 1998 Jan 20;95(2):525-30. 9435225 Chan DW, Ye R, Veillette CJ, Lees-Miller SP: DNA-dependent protein kinase phosphorylation sites in Ku 70/80 heterodimer. Biochemistry. 1999 Feb 9;38(6):1819-28. 10026262 Ariumi Y, Masutani M, Copeland TD, Mimori T, Sugimura T, Shimotohno K, Ueda K, Hatanaka M, Noda M: Suppression of the poly(ADP-ribose) polymerase activity by DNA-dependent protein kinase in vitro. Oncogene. 1999 Aug 12;18(32):4616-25. 10467406 Douglas P, Sapkota GP, Morrice N, Yu Y, Goodarzi AA, Merkle D, Meek K, Alessi DR, Lees-Miller SP: Identification of in vitro and in vivo phosphorylation sites in the catalytic subunit of the DNA-dependent protein kinase. Biochem J. 2002 Nov 15;368(Pt 1):243-51. 12186630 Ma Y, Pannicke U, Schwarz K, Lieber MR: Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. Cell. 2002 Mar 22;108(6):781-94. 11955432 Hsu HL, Yannone SM, Chen DJ: Defining interactions between DNA-PK and ligase IV/XRCC4. DNA Repair (Amst). 2002 Mar 28;1(3):225-35. 12509254 Chan DW, Chen BP, Prithivirajsingh S, Kurimasa A, Story MD, Qin J, Chen DJ: Autophosphorylation of the DNA-dependent protein kinase catalytic subunit is required for rejoining of DNA double-strand breaks. Genes Dev. 2002 Sep 15;16(18):2333-8. 12231622 Karmakar P, Piotrowski J, Brosh RM Jr, Sommers JA, Miller SP, Cheng WH, Snowden CM, Ramsden DA, Bohr VA: Werner protein is a target of DNA-dependent protein kinase in vivo and in vitro, and its catalytic activities are regulated by phosphorylation. J Biol Chem. 2002 May 24;277(21):18291-302. Epub 2002 Mar 11. 11889123 Soubeyrand S, Pope L, Pakuts B, Hache RJ: Threonines 2638/2647 in DNA-PK are essential for cellular resistance to ionizing radiation. Cancer Res. 2003 Mar 15;63(6):1198-201. 12649176 Schild-Poulter C, Shih A, Yarymowich NC, Hache RJ: Down-regulation of histone H2B by DNA-dependent protein kinase in response to DNA damage through modulation of octamer transcription factor 1. Cancer Res. 2003 Nov 1;63(21):7197-205. 14612514 Park EJ, Chan DW, Park JH, Oettinger MA, Kwon J: DNA-PK is activated by nucleosomes and phosphorylates H2AX within the nucleosomes in an acetylation-dependent manner. Nucleic Acids Res. 2003 Dec 1;31(23):6819-27. 14627815 Pannicke U, Ma Y, Hopfner KP, Niewolik D, Lieber MR, Schwarz K: Functional and biochemical dissection of the structure-specific nuclease ARTEMIS. EMBO J. 2004 May 5;23(9):1987-97. Epub 2004 Apr 8. 15071507 Poinsignon C, Moshous D, Callebaut I, de Chasseval R, Villey I, de Villartay JP: The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination. J Exp Med. 2004 Feb 2;199(3):315-21. Epub 2004 Jan 26. 14744996 Ma Y, Lu H, Tippin B, Goodman MF, Shimazaki N, Koiwai O, Hsieh CL, Schwarz K, Lieber MR: A biochemically defined system for mammalian nonhomologous DNA end joining. Mol Cell. 2004 Dec 3;16(5):701-13. 15574326 Zhang X, Succi J, Feng Z, Prithivirajsingh S, Story MD, Legerski RJ: Artemis is a phosphorylation target of ATM and ATR and is involved in the G2/M DNA damage checkpoint response. Mol Cell Biol. 2004 Oct;24(20):9207-20. 15456891 Zhang S, Schlott B, Gorlach M, Grosse F: DNA-dependent protein kinase (DNA-PK) phosphorylates nuclear DNA helicase II/RNA helicase A and hnRNP proteins in an RNA-dependent manner. Nucleic Acids Res. 2004 Jan 2;32(1):1-10. Print 2004. 14704337 Wechsler T, Chen BP, Harper R, Morotomi-Yano K, Huang BC, Meek K, Cleaver JE, Chen DJ, Wabl M: DNA-PKcs function regulated specifically by protein phosphatase 5. Proc Natl Acad Sci U S A. 2004 Feb 3;101(5):1247-52. Epub 2004 Jan 20. 14734805 Reitsema T, Klokov D, Banath JP, Olive PL: DNA-PK is responsible for enhanced phosphorylation of histone H2AX under hypertonic conditions. DNA Repair (Amst). 2005 Sep 28;4(10):1172-81. 16046194 Wang J, Pluth JM, Cooper PK, Cowan MJ, Chen DJ, Yannone SM: Artemis deficiency confers a DNA double-strand break repair defect and Artemis phosphorylation status is altered by DNA damage and cell cycle progression. DNA Repair (Amst). 2005 May 2;4(5):556-70. 15811628 Ma Y, Schwarz K, Lieber MR: The Artemis:DNA-PKcs endonuclease cleaves DNA loops, flaps, and gaps. DNA Repair (Amst). 2005 Jul 12;4(7):845-51. 15936993 Falck J, Coates J, Jackson SP: Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature. 2005 Mar 31;434(7033):605-11. Epub 2005 Mar 2. 15758953 Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006 Nov 3;127(3):635-48. 17081983 Levy N, Martz A, Bresson A, Spenlehauer C, de Murcia G, Menissier-de Murcia J: XRCC1 is phosphorylated by DNA-dependent protein kinase in response to DNA damage. Nucleic Acids Res. 2006 Jan 5;34(1):32-41. Print 2006. 16397295 Collis SJ, DeWeese TL, Jeggo PA, Parker AR: The life and death of DNA-PK. Oncogene. 2005 Feb 3;24(6):949-61. 15592499 Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER 3rd, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ: ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science. 2007 May 25;316(5828):1160-6. 17525332 Daub H, Olsen JV, Bairlein M, Gnad F, Oppermann FS, Korner R, Greff Z, Keri G, Stemmann O, Mann M: Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell. 2008 Aug 8;31(3):438-48. doi: 10.1016/j.molcel.2008.07.007. 18691976 Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31. 18669648 van der Burg M, Ijspeert H, Verkaik NS, Turul T, Wiegant WW, Morotomi-Yano K, Mari PO, Tezcan I, Chen DJ, Zdzienicka MZ, van Dongen JJ, van Gent DC: A DNA-PKcs mutation in a radiosensitive T-B- SCID patient inhibits Artemis activation and nonhomologous end-joining. J Clin Invest. 2009 Jan;119(1):91-8. doi: 10.1172/JCI37141. Epub 2008 Dec 15. 19075392 Oppermann FS, Gnad F, Olsen JV, Hornberger R, Greff Z, Keri G, Mann M, Daub H: Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics. 2009 Jul;8(7):1751-64. doi: 10.1074/mcp.M800588-MCP200. Epub 2009 Apr 15. 19369195 Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal. 2009 Aug 18;2(84):ra46. doi: 10.1126/scisignal.2000007. 19690332 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M: Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science. 2009 Aug 14;325(5942):834-40. doi: 10.1126/science.1175371. Epub 2009 Jul 16. 19608861 Hurov KE, Cotta-Ramusino C, Elledge SJ: A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability. Genes Dev. 2010 Sep 1;24(17):1939-50. doi: 10.1101/gad.1934210. 20810650 Takai H, Xie Y, de Lange T, Pavletich NP: Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes. Genes Dev. 2010 Sep 15;24(18):2019-30. doi: 10.1101/gad.1956410. Epub 2010 Aug 27. 20801936 Kaizuka T, Hara T, Oshiro N, Kikkawa U, Yonezawa K, Takehana K, Iemura S, Natsume T, Mizushima N: Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly. J Biol Chem. 2010 Jun 25;285(26):20109-16. doi: 10.1074/jbc.M110.121699. Epub 2010 Apr 28. 20427287 Olsen JV, Vermeulen M, Santamaria A, Kumar C, Miller ML, Jensen LJ, Gnad F, Cox J, Jensen TS, Nigg EA, Brunak S, Mann M: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal. 2010 Jan 12;3(104):ra3. doi: 10.1126/scisignal.2000475. 20068231 Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17. 21269460 So EY, Ouchi T: Functional interaction of BRCA1/ATM-associated BAAT1 with the DNA-PK catalytic subunit. Exp Ther Med. 2011 May;2(3):443-447. Epub 2011 Mar 21. 22977523 Rigbolt KT, Prokhorova TA, Akimov V, Henningsen J, Johansen PT, Kratchmarova I, Kassem M, Mann M, Olsen JV, Blagoev B: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal. 2011 Mar 15;4(164):rs3. doi: 10.1126/scisignal.2001570. 21406692 Ahmad Y, Boisvert FM, Lundberg E, Uhlen M, Lamond AI: Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization. Mol Cell Proteomics. 2012 Mar;11(3):M111.013680. doi: 10.1074/mcp.M111.013680. Epub 2011 Oct 16. 22002106 Yuce O, West SC: Senataxin, defective in the neurodegenerative disorder ataxia with oculomotor apraxia 2, lies at the interface of transcription and the DNA damage response. Mol Cell Biol. 2013 Jan;33(2):406-17. doi: 10.1128/MCB.01195-12. Epub 2012 Nov 12. 23149945 Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. 24275569 Ho SR, Mahanic CS, Lee YJ, Lin WC: RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage. Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):E2646-55. doi: 10.1073/pnas.1323107111. Epub 2014 Jun 16. 24979766 Medunjanin S, Daniel JM, Weinert S, Dutzmann J, Burgbacher F, Brecht S, Bruemmer D, Kahne T, Naumann M, Sedding DG, Zuschratter W, Braun-Dullaeus RC: DNA-dependent protein kinase (DNA-PK) permits vascular smooth muscle cell proliferation through phosphorylation of the orphan nuclear receptor NOR1. Cardiovasc Res. 2015 Jun 1;106(3):488-97. doi: 10.1093/cvr/cvv126. Epub 2015 Apr 7. 25852083 Craxton A, Somers J, Munnur D, Jukes-Jones R, Cain K, Malewicz M: XLS (c9orf142) is a new component of mammalian DNA double-stranded break repair. Cell Death Differ. 2015 Jun;22(6):890-7. doi: 10.1038/cdd.2015.22. Epub 2015 Mar 13. 25941166 Xing M, Yang M, Huo W, Feng F, Wei L, Jiang W, Ning S, Yan Z, Li W, Wang Q, Hou M, Dong C, Guo R, Gao G, Ji J, Zha S, Lan L, Liang H, Xu D: Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway. Nat Commun. 2015 Feb 11;6:6233. doi: 10.1038/ncomms7233. 25670504 Vaca Jacome AS, Rabilloud T, Schaeffer-Reiss C, Rompais M, Ayoub D, Lane L, Bairoch A, Van Dorsselaer A, Carapito C: N-terminome analysis of the human mitochondrial proteome. Proteomics. 2015 Jul;15(14):2519-24. doi: 10.1002/pmic.201400617. Epub 2015 Jun 8. 25944712 Ochi T, Blackford AN, Coates J, Jhujh S, Mehmood S, Tamura N, Travers J, Wu Q, Draviam VM, Robinson CV, Blundell TL, Jackson SP: DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair. Science. 2015 Jan 9;347(6218):185-8. doi: 10.1126/science.1261971. 25574025 Greenman C, Stephens P, Smith R, Dalgliesh GL, Hunter C, Bignell G, Davies H, Teague J, Butler A, Stevens C, Edkins S, O'Meara S, Vastrik I, Schmidt EE, Avis T, Barthorpe S, Bhamra G, Buck G, Choudhury B, Clements J, Cole J, Dicks E, Forbes S, Gray K, Halliday K, Harrison R, Hills K, Hinton J, Jenkinson A, Jones D, Menzies A, Mironenko T, Perry J, Raine K, Richardson D, Shepherd R, Small A, Tofts C, Varian J, Webb T, West S, Widaa S, Yates A, Cahill DP, Louis DN, Goldstraw P, Nicholson AG, Brasseur F, Looijenga L, Weber BL, Chiew YE, DeFazio A, Greaves MF, Green AR, Campbell P, Birney E, Easton DF, Chenevix-Trench G, Tan MH, Khoo SK, Teh BT, Yuen ST, Leung SY, Wooster R, Futreal PA, Stratton MR: Patterns of somatic mutation in human cancer genomes. Nature. 2007 Mar 8;446(7132):153-8. 17344846 Woodbine L, Neal JA, Sasi NK, Shimada M, Deem K, Coleman H, Dobyns WB, Ogi T, Meek K, Davies EG, Jeggo PA: PRKDC mutations in a SCID patient with profound neurological abnormalities. J Clin Invest. 2013 Jul;123(7):2969-80. doi: 10.1172/JCI67349. Epub 2013 Jun 3. 23722905