Potassium-transporting ATPase alpha chain 2

NamePotassium-transporting ATPase alpha chain 2
Synonyms
  • 3.6.3.10
  • ATP1AL1
  • Non-gastric H(+)/K(+) ATPase subunit alpha
  • Proton pump
Gene NameATP12A
OrganismHuman
Amino acid sequence
>lcl|BSEQ0037108|Potassium-transporting ATPase alpha chain 2
MHQKTPEIYSVELSGTKDIVKTDKGDGKEKYRGLKNNCLELKKKNHKEEFQKELHLDDHK
LSNRELEEKYGTDIIMGLSSTRAAELLARDGPNSLTPPKQTPEIVKFLKQMVGGFSILLW
VGAFLCWIAYGIQYSSDKSASLNNVYLGCVLGLVVILTGIFAYYQEAKSTNIMSSFNKMI
PQQALVIRDSEKKTIPSEQLVVGDIVEVKGGDQIPADIRVLSSQGCRVDNSSLTGESEPQ
PRSSEFTHENPLETKNICFYSTTCLEGTVTGMVINTGDRTIIGHIASLASGVGNEKTPIA
IEIEHFVHIVAGVAVSIGILFFIIAVSLKYQVLDSIIFLIGIIVANVPEGLLATVTVTLS
LTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLWFDNQIFVADTSED
HSNQVFDQSSRTWASLSKIITLCNRAEFKPGQENVPIMKKAVIGDASETALLKFSEVILG
DVMEIRKRNRKVAEIPFNSTNKFQLSIHEMDDPHGKRFLMVMKGAPERILEKCSTIMING
EEHPLDKSTAKTFHTAYMELGGLGERVLGFCHLYLPADEFPETYSFDIDAMNFPTSNLCF
VGLLSMIDPPRSTVPDAVTKCRSAGIKVIMVTGDHPITAKAIAKSVGIISANSETVEDIA
HRLNIAVEQVNKRDAKAAVVTGMELKDMSSEQLDEILANYQEIVFARTSPQQKLIIVEGC
QRQDAVVAVTGDGVNDSPALKKADIGIAMGIAGSDAAKNAADMVLLDDNFASIVTGVEEG
RLIFDNLKKTIAYSLTKNIAELCPFLIYIIVGLPLPIGTITILFIDLGTDIIPSIALAYE
KAESDIMNRKPRHKNKDRLVNQPLAVYSYLHIGLMQALGAFLVYFTVYAQEGFLPRTLIN
LRVEWEKDYVNDLKDSYGQEWTRYQREYLEWTGYTAFFVGILVQQIADLIIRKTRRNSIF
QQGLFRNKVIWVGITSQIIIGLILSYGLGSVTALSFTMLRAQYWFVAVPHAILIWVYDEV
RKLFIRLYPGSWWDKNMYY
Number of residues1039
Molecular Weight115509.45
Theoretical pI6.52
GO Classification
Functions
  • ATP binding
  • metal ion binding
  • sodium
  • hydrogen
Processes
  • cellular sodium ion homeostasis
  • response to organic cyclic compound
  • regulation of pH
  • response to metal ion
  • transmembrane transport
  • ion transmembrane transport
  • potassium ion import
  • establishment or maintenance of transmembrane electrochemical gradient
  • sodium ion export from cell
  • cellular potassium ion homeostasis
  • ATP hydrolysis coupled proton transport
Components
  • plasma membrane
  • hydrogen
  • intracellular membrane-bounded organelle
  • basolateral plasma membrane
  • apical plasma membrane
General FunctionSodium:potassium-exchanging atpase activity
Specific FunctionCatalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for potassium absorption in various tissues.
Pfam Domain Function
Transmembrane Regions103-123 147-167 304-323 336-353 788-807 818-838 859-881 934-953 968-986 1002-1022
GenBank Protein ID404017
UniProtKB IDP54707
UniProtKB Entry NameAT12A_HUMAN
Cellular LocationMembrane
Gene sequence
>lcl|BSEQ0010684|Potassium-transporting ATPase alpha chain 2 (ATP12A)
ATGCACCAGAAAACCCCAGAAATTTACTCCGTGGAGCTCAGCGGAACTAAGGACATCGTG
AAAACAGACAAGGGGGATGGCAAGGAGAAGTATAGGGGTCTGAAGAACAACTGCCTGGAA
CTCAAAAAGAAAAATCACAAAGAGGAGTTTCAGAAAGAACTCCATCTGGATGACCACAAA
CTCAGCAATAGGGAATTGGAAGAGAAATATGGCACAGACATCATTATGGGTCTCTCCAGC
ACCAGAGCTGCCGAGCTCCTGGCCCGGGATGGGCCCAACTCCCTCACCCCTCCCAAGCAG
ACGCCTGAGATCGTCAAGTTCCTCAAGCAGATGGTGGGGGGGTTCTCTATCCTCCTGTGG
GTGGGCGCCTTTCTCTGTTGGATTGCATATGGGATTCAGTACTCCAGCGACAAGTCTGCA
TCCCTGAACAACGTGTACTTGGGCTGTGTGCTTGGTCTGGTGGTCATTTTAACGGGGATC
TTTGCTTATTACCAAGAGGCAAAAAGCACCAACATCATGTCCAGCTTCAATAAGATGATC
CCTCAGCAAGCTCTCGTCATCCGAGATTCCGAGAAGAAGACCATCCCTTCAGAGCAGCTG
GTGGTGGGGGACATTGTGGAGGTCAAAGGAGGAGACCAGATCCCTGCAGACATCAGGGTG
CTGTCTTCTCAGGGGTGTCGGGTGGATAACTCATCTCTCACGGGGGAGTCTGAGCCCCAG
CCCCGCTCCTCTGAGTTTACCCATGAAAACCCCCTGGAAACAAAGAACATCTGCTTCTAT
TCCACAACGTGTCTGGAAGCATCTACTTCCCCTGTAGGCACTGTCACCGGCATGGTTATC
AACACGGGTGACCGCACCATCATTGGCCATATTGCCTCATTGGCCTCAGGAGTTGGAAAT
GAGAAGACGCCCATTGCCATTGAGATCGAGCACTTTGTTCACATTGTGGCAGGAGTGGCT
GTCTCCATCGGCATCCTTTTCTTCATCATCGCTGTGTCCCTGAAGTATCAAGTCCTGGAC
TCCATCATCTTCCTCATTGGCATCATTGTGGCCAATGTGCCCGAGGGCCTCCTGGCCACT
GTCACTGTGACCCTGTCGCTGACAGCAAAACGGATGGCCAAGAAGAACTGCCTGGTGAAG
AACCTGGAGGCTGTGGAGACCCTCGGCTCCACCTCCATCATCTGCTCGGACAAGACTGGG
ACACTGACCCAGAACAGGATGACAGTGGCCCATCTGTGGTTCGACAATCAGATCTTTGTG
GCTGACACCAGTGAGGACCATTCAAACCAAGTCTTTGACCAAAGCTCTAGGACTTGGGCC
TCCTTATCCAAGATAATAACATTGTGTAACCGAGCAGAGTTCAAGCCAGGACAGGAAAAT
GTCCCCATCATGAAGAAAGCTGTGATTGGAGATGCCTCAGAAACTGCTCTTTTAAAATTC
TCAGAGGTCATTTTGGGTGATGTGATGGAAATTAGAAAAAGAAACCGCAAAGTAGCTGAA
ATCCCTTTTAACTCTACTAATAAATTTCAGCTCTCCATCCACGAGATGGATGACCCCCAC
GGCAAGCGCTTCCTCATGGTGATGAAGGGGGCCCCTGAGCGCATCCTAGAGAAATGCAGC
ACCATCATGATCAACGGCGAGGAGCACCCACTGGACAAGAGCACTGCCAAGACCTTCCAC
ACAGCCTACATGGAGCTGGGCGGGTTGGGCGAGCGTGTGCTGGGTTTCTGTCATCTCTAC
CTGCCAGCAGACGAGTTTCCAGAAACCTACTCATTTGACATAGACGCTATGAACTTTCCG
ACCTCCAACCTCTGTTTTGTGGGACTCTTGTCAATGATCGATCCCCCTCGGTCCACCGTG
CCAGATGCAGTCACCAAATGCCGGAGTGCAGGGATCAAGGTTATTATGGTTACTGGTGAT
CATCCCATCACAGCCAAAGCTATTGCCAAGAGTGTGGGGATCATTTCAGCCAACAGTGAA
ACAGTGGAAGACATTGCACATCGCCTCAACATTGCTGTGGAGCAAGTTAACAAACGGGAT
GCCAAGGCCGCTGTGGTGACTGGCATGGAGCTGAAGGACATGAGCTCAGAACAGCTGGAT
GAGATCTTAGCCAACTACCAGGAGATTGTCTTTGCCCGGACATCCCCCCAGCAGAAGCTG
ATCATTGTGGAGGGCTGTCAGAGGCAGGATGCTGTTGTTGCTGTGACCGGGGATGGAGTT
AATGACTCTCCGGCTCTAAAGAAGGCAGACATTGGGATTGCCATGGGGATAGCAGGTTCT
GATGCAGCCAAAAATGCAGCCGACATGGTCTTGCTGGACGACAACTTCGCATCCATCGTC
ACAGGGGTGGAGGAAGGTCGCCTGATCTTTGACAACCTCAAGAAGACTATTGCTTATTCC
CTGACCAAGAACATTGCCGAGCTGTGCCCCTTTCTGATCTACATCATTGTCGGGCTCCCC
CTGCCCATTGGCACCATCACCATTCTGTTCATTGACTTGGGGACAGACATTATCCCCTCC
ATTGCCTTGGCGTACGAGAAAGCTGAAAGTGACATCATGAACAGGAAGCCTCGCCACAAG
AATAAGGACAGGCTGGTGAACCAGCCGCTCGCTGTGTACTCATACCTGCACATTGGCCTC
ATGCAAGCCCTGGGAGCTTTCCTTGTGTATTTCACCGTCTATGCACAAGAGGGCTTTCTG
CCCCGCACTCTCATTAACCTGCGGGTAGAATGGGAGAAGGACTACGTGAATGACTTGAAA
GACAGCTATGGGCAGGAATGGACAAGGTACCAGAGGGAATACCTAGAATGGACGGGCTAC
ACGGCTTTCTTTGTTGGCATCCTAGTCCAGCAAATAGCAGATCTGATCATCAGGAAAACC
CGGAGGAATTCCATCTTCCAGCAGGGTCTCTTCAGAAATAAAGTCATCTGGGTGGGGATC
ACCTCACAGATCATCATTGGTCTGATCCTCTCCTATGGCCTCGGAAGTGTCACAGCCTTG
AGTTTCACCATGCTTAGGGCTCAGTACTGGTTTGTGGCTGTGCCGCACGCCATCCTGATC
TGGGTGTATGATGAGGTGCGGAAGCTCTTCATCAGGCTCTACCCTGGAAGCTGGTGGGAT
AAGAACATGTATTATTAA
GenBank Gene IDU02076
GeneCard IDNot Available
GenAtlas IDATP12A
HGNC IDHGNC:13816
Chromosome Location13
Locus13q12.12|13q12.1-q12.3
References
  1. Grishin AV, Sverdlov VE, Kostina MB, Modyanov NN: Cloning and characterization of the entire cDNA encoded by ATP1AL1--a member of the human Na,K/H,K-ATPase gene family. FEBS Lett. 1994 Jul 25;349(1):144-50. 8045293
  2. Sverdlov VE, Kostina MB, Modyanov NN: Genomic organization of the human ATP1AL1 gene encoding a ouabain-sensitive H,K-ATPase. Genomics. 1996 Mar 15;32(3):317-27. 8838794
  3. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. 14702039
  4. Dunham A, Matthews LH, Burton J, Ashurst JL, Howe KL, Ashcroft KJ, Beare DM, Burford DC, Hunt SE, Griffiths-Jones S, Jones MC, Keenan SJ, Oliver K, Scott CE, Ainscough R, Almeida JP, Ambrose KD, Andrews DT, Ashwell RI, Babbage AK, Bagguley CL, Bailey J, Bannerjee R, Barlow KF, Bates K, Beasley H, Bird CP, Bray-Allen S, Brown AJ, Brown JY, Burrill W, Carder C, Carter NP, Chapman JC, Clamp ME, Clark SY, Clarke G, Clee CM, Clegg SC, Cobley V, Collins JE, Corby N, Coville GJ, Deloukas P, Dhami P, Dunham I, Dunn M, Earthrowl ME, Ellington AG, Faulkner L, Frankish AG, Frankland J, French L, Garner P, Garnett J, Gilbert JG, Gilson CJ, Ghori J, Grafham DV, Gribble SM, Griffiths C, Hall RE, Hammond S, Harley JL, Hart EA, Heath PD, Howden PJ, Huckle EJ, Hunt PJ, Hunt AR, Johnson C, Johnson D, Kay M, Kimberley AM, King A, Laird GK, Langford CJ, Lawlor S, Leongamornlert DA, Lloyd DM, Lloyd C, Loveland JE, Lovell J, Martin S, Mashreghi-Mohammadi M, McLaren SJ, McMurray A, Milne S, Moore MJ, Nickerson T, Palmer SA, Pearce AV, Peck AI, Pelan S, Phillimore B, Porter KM, Rice CM, Searle S, Sehra HK, Shownkeen R, Skuce CD, Smith M, Steward CA, Sycamore N, Tester J, Thomas DW, Tracey A, Tromans A, Tubby B, Wall M, Wallis JM, West AP, Whitehead SL, Willey DL, Wilming L, Wray PW, Wright MW, Young L, Coulson A, Durbin R, Hubbard T, Sulston JE, Beck S, Bentley DR, Rogers J, Ross MT: The DNA sequence and analysis of human chromosome 13. Nature. 2004 Apr 1;428(6982):522-8. 15057823
  5. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. 15489334
  6. Modyanov NN, Petrukhin KE, Sverdlov VE, Grishin AV, Orlova MY, Kostina MB, Makarevich OI, Broude NE, Monastyrskaya GS, Sverdlov ED: The family of human Na,K-ATPase genes. ATP1AL1 gene is transcriptionally competent and probably encodes the related ion transport ATPase. FEBS Lett. 1991 Jan 14;278(1):91-4. 1847115
  7. Shull MM, Lingrel JB: Multiple genes encode the human Na+,K+-ATPase catalytic subunit. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4039-43. 3035563
  8. Sverdlov ED, Monastyrskaya GS, Broude NE, Ushkaryov YuA, Allikmets RL, Melkov AM, Smirnov YuV, Malyshev IV, Dulobova IE, Petrukhin KE, et al.: The family of human Na+,K+-ATPase genes. No less than five genes and/or pseudogenes related to the alpha-subunit. FEBS Lett. 1987 Jun 15;217(2):275-8. 3036582
  9. Pestov NB, Romanova LG, Korneenko TV, Egorov MV, Kostina MB, Sverdlov VE, Askari A, Shakhparonov MI, Modyanov NN: Ouabain-sensitive H,K-ATPase: tissue-specific expression of the mammalian genes encoding the catalytic alpha subunit. FEBS Lett. 1998 Dec 4;440(3):320-4. 9872395
  10. Streif D, Iglseder E, Hauser-Kronberger C, Fink KG, Jakab M, Ritter M: Expression of the non-gastric H+/K+ ATPase ATP12A in normal and pathological human prostate tissue. Cell Physiol Biochem. 2011;28(6):1287-94. doi: 10.1159/000335860. Epub 2011 Dec 16. 22179016
  11. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. 24275569