NameMethionine aminopeptidase 1
Synonyms
  • 3.4.11.18
  • KIAA0094
  • MAP 1
  • Peptidase M 1
Gene NameMETAP1
OrganismHuman
Amino acid sequence
>lcl|BSEQ0007849|Methionine aminopeptidase 1
MAAVETRVCETDGCSSEAKLQCPTCIKLGIQGSYFCSQECFKGSWATHKLLHKKAKDEKA
KREVSSWTVEGDINTDPWAGYRYTGKLRPHYPLMPTRPVPSYIQRPDYADHPLGMSESEQ
ALKGTSQIKLLSSEDIEGMRLVCRLAREVLDVAAGMIKPGVTTEEIDHAVHLACIARNCY
PSPLNYYNFPKSCCTSVNEVICHGIPDRRPLQEGDIVNVDITLYRNGYHGDLNETFFVGE
VDDGARKLVQTTYECLMQAIDAVKPGVRYRELGNIIQKHAQANGFSVVRSYCGHGIHKLF
HTAPNVPHYAKNKAVGVMKSGHVFTIEPMICEGGWQDETWPDGWTAVTRDGKRSAQFEHT
LLVTDTGCEILTRRLDSARPHFMSQF
Number of residues386
Molecular Weight43214.885
Theoretical pI7.18
GO Classification
Functions
  • metalloexopeptidase activity
  • metal ion binding
  • aminopeptidase activity
Processes
  • phototransduction, visible light
  • platelet aggregation
  • regulation of translation
  • N-terminal protein amino acid modification
  • peptidyl-methionine modification
  • regulation of rhodopsin mediated signaling pathway
  • rhodopsin mediated signaling pathway
Components
  • cytosol
  • cytoplasm
  • nucleus
General FunctionMetalloexopeptidase activity
Specific FunctionCotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal progression through the cell cycle.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID577315
UniProtKB IDP53582
UniProtKB Entry NameMAP11_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0019522|Methionine aminopeptidase 1 (METAP1)
ATGGCGGCCGTGGAGACGCGGGTGTGCGAGACAGACGGCTGCAGCAGTGAGGCCAAGCTC
CAGTGTCCCACTTGCATCAAGCTGGGCATCCAGGGCTCGTACTTCTGCTCGCAGGAATGT
TTTAAAGGAAGTTGGGCTACTCACAAGTTACTACATAAGAAAGCAAAAGATGAAAAGGCG
AAGCGAGAAGTGTCTTCCTGGACTGTGGAAGGTGATATTAATACTGACCCATGGGCAGGT
TATCGATATACTGGTAAACTCAGACCACATTATCCACTGATGCCAACAAGGCCAGTGCCA
AGTTATATTCAAAGACCAGATTATGCTGATCATCCCTTAGGAATGTCTGAATCTGAACAG
GCTCTTAAAGGTACTTCTCAGATTAAATTACTCTCATCTGAAGATATAGAAGGGATGCGA
CTTGTATGTAGGCTTGCTAGAGAAGTTTTGGATGTTGCTGCCGGCATGATTAAACCAGGT
GTAACTACTGAAGAAATAGATCACGCTGTACACTTAGCATGTATTGCAAGAAATTGCTAC
CCTTCTCCCCTGAATTATTATAATTTCCCAAAGTCTTGTTGTACCTCAGTGAATGAAGTC
ATTTGCCATGGAATACCAGACAGAAGGCCCTTACAAGAAGGTGACATTGTTAATGTGGAT
ATCACTCTTTATCGCAATGGTTATCATGGGGACCTGAATGAGACATTTTTTGTTGGAGAA
GTGGATGATGGAGCACGGAAACTTGTTCAGACCACATATGAGTGCCTGATGCAAGCCATT
GATGCAGTGAAGCCTGGTGTTCGGTACAGAGAATTGGGAAACATTATCCAGAAGCATGCC
CAAGCAAATGGGTTTTCAGTTGTTCGAAGCTATTGTGGGCATGGAATCCACAAGCTTTTT
CATACAGCTCCCAATGTACCCCACTATGCTAAAAATAAAGCAGTTGGAGTGATGAAGTCG
GGCCATGTATTTACAATTGAGCCAATGATTTGTGAAGGCGGATGGCAGGATGAAACCTGG
CCAGATGGTTGGACTGCGGTGACAAGAGACGGAAAGCGGTCTGCTCAGTTTGAGCACACC
CTCCTGGTCACAGACACTGGCTGTGAAATCCTAACCCGGCGACTTGACAGTGCACGGCCT
CACTTCATGTCTCAATTTTAA
GenBank Gene IDD42084
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:15789
Chromosome Location4
Locus4q23
References
  1. Nagase T, Miyajima N, Tanaka A, Sazuka T, Seki N, Sato S, Tabata S, Ishikawa K, Kawarabayasi Y, Kotani H, et al.: Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 1995;2(1):37-43. 7788527
  2. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. 14702039
  3. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. 15489334
  4. Gauci S, Helbig AO, Slijper M, Krijgsveld J, Heck AJ, Mohammed S: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem. 2009 Jun 1;81(11):4493-501. doi: 10.1021/ac9004309. 19413330
  5. Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17. 21269460
  6. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. 24275569
  7. Vaca Jacome AS, Rabilloud T, Schaeffer-Reiss C, Rompais M, Ayoub D, Lane L, Bairoch A, Van Dorsselaer A, Carapito C: N-terminome analysis of the human mitochondrial proteome. Proteomics. 2015 Jul;15(14):2519-24. doi: 10.1002/pmic.201400617. Epub 2015 Jun 8. 25944712
  8. Addlagatta A, Hu X, Liu JO, Matthews BW: Structural basis for the functional differences between type I and type II human methionine aminopeptidases. Biochemistry. 2005 Nov 15;44(45):14741-9. 16274222
  9. Hu X, Addlagatta A, Matthews BW, Liu JO: Identification of pyridinylpyrimidines as inhibitors of human methionine aminopeptidases. Angew Chem Int Ed Engl. 2006 Jun 2;45(23):3772-5. 16724298
  10. Hu X, Addlagatta A, Lu J, Matthews BW, Liu JO: Elucidation of the function of type 1 human methionine aminopeptidase during cell cycle progression. Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18148-53. Epub 2006 Nov 17. 17114291
  11. Addlagatta A, Matthews BW: Structure of the angiogenesis inhibitor ovalicin bound to its noncognate target, human Type 1 methionine aminopeptidase. Protein Sci. 2006 Aug;15(8):1842-8. Epub 2006 Jul 5. 16823043