MAP kinase-activated protein kinase 2

NameMAP kinase-activated protein kinase 2
Synonyms
  • 2.7.11.1
  • MAPK-activated protein kinase 2
Gene NameMAPKAPK2
OrganismHuman
Amino acid sequence
>lcl|BSEQ0002725|MAP kinase-activated protein kinase 2
MLSNSQGQSPPVPFPAPAPPPQPPTPALPHPPAQPPPPPPQQFPQFHVKSGLQIKKNAII
DDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRI
VDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSI
NIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPCYTPYYVAPEVLGPEKY
DKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKM
LIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSAL
ATMRVDYEQIKIKKIEDASNPLLLKRRKKARALEAAALAH
Number of residues400
Molecular Weight45567.415
Theoretical pI8.92
GO Classification
Functions
  • calmodulin binding
  • calmodulin-dependent protein kinase activity
  • signal transducer activity
  • calcium-dependent protein serine/threonine kinase activity
  • ATP binding
  • protein kinase activity
  • protein serine/threonine kinase activity
  • mitogen-activated protein kinase binding
Processes
  • innate immune response
  • TRIF-dependent toll-like receptor signaling pathway
  • MAPK cascade
  • positive regulation of tumor necrosis factor biosynthetic process
  • neurotrophin TRK receptor signaling pathway
  • G2 DNA damage checkpoint
  • cellular response to heat
  • protein phosphorylation
  • MyD88-dependent toll-like receptor signaling pathway
  • Ras protein signal transduction
  • macropinocytosis
  • MyD88-independent toll-like receptor signaling pathway
  • vascular endothelial growth factor receptor signaling pathway
  • p38MAPK cascade
  • regulation of cellular response to heat
  • response to lipopolysaccharide
  • regulation of tumor necrosis factor production
  • stress-activated MAPK cascade
  • inner ear development
  • inflammatory response
  • toll-like receptor 10 signaling pathway
  • protein autophosphorylation
  • response to stress
  • toll-like receptor 2 signaling pathway
  • toll-like receptor 3 signaling pathway
  • activation of MAPK activity
  • gene expression
  • toll-like receptor 4 signaling pathway
  • 3'-UTR-mediated mRNA stabilization
  • arachidonic acid metabolic process
  • small molecule metabolic process
  • toll-like receptor 5 signaling pathway
  • regulation of interleukin-6 production
  • leukotriene metabolic process
  • toll-like receptor 9 signaling pathway
  • response to cytokine
  • toll-like receptor signaling pathway
  • peptidyl-serine phosphorylation
  • toll-like receptor TLR1
  • cellular response to vascular endothelial growth factor stimulus
  • cellular response to DNA damage stimulus
  • toll-like receptor TLR6
  • regulation of mRNA stability
Components
  • centrosome
  • cytoplasm
  • nucleus
  • cytosol
  • extracellular exosome
  • nucleoplasm
General FunctionSignal transducer activity
Specific FunctionStress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilize GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID530090
UniProtKB IDP49137
UniProtKB Entry NameMAPK2_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0021291|MAP kinase-activated protein kinase 2 (MAPKAPK2)
ATGCTGTCCAACTCCCAGGGCCAGAGCCCGCCGGTGCCGTTCCCCGCCCCGGCCCCGCCG
CCGCAGCCCCCCACCCCTGCCCTGCCGCACCCCCCGGCGCAGCCGCCGCCGCCGCCCCCG
CAGCAGTTCCCGCAGTTCCACGTCAAGTCCGGCCTGCAGATCAAGAAGAACGCCATCATC
GATGACTACAAGGTCACCAGCCAGGTCCTGGGGCTGGGCATCAACGGCAAAGTTTTGCAG
ATCTTCAACAAGAGGACCCAGGAGAAATTCGCCCTCAAAATGCTTCAGGACTGCCCCAAG
GCCCGCAGGGAGGTGGAGCTGCACTGGCGGGCCTCCCAGTGCCCGCACATCGTACGGATC
GTGGATGTGTACGAGAATCTGTACGCAGGGAGGAAGTGCCTGCTGATTGTCATGGAATGT
TTGGACGGTGGAGAACTCTTTAGCCGAATCCAGGATCGAGGAGACCAGGCATTCACAGAA
AGAGAAGCATCCGAAATCATGAAGAGCATCGGTGAGGCCATCCAGTATCTGCATTCAATC
AACATTGCCCATCGGGATGTCAAGCCTGAGAATCTCTTATACACCTCCAAAAGGCCCAAC
GCCATCCTGAAACTCACTGACTTTGGCTTTGCCAAGGAAACCACCAGCCACAACTCTTTG
ACCACTCCTTGTTATACACCGTACTATGTGGCTCCAGAAGTGCTGGGTCCAGAGAAGTAT
GACAAGTCCTGTGACATGTGGTCCCTGGGTGTCATCATGTACATCCTGCTGTGTGGGTAT
CCCCCCTTCTACTCCAACCACGGCCTTGCCATCTCTCCGGGCATGAAGACTCGCATCCGA
ATGGGCCAGTATGAATTTCCCAACCCAGAATGGTCAGAAGTATCAGAGGAAGTGAAGATG
CTCATTCGGAATCTGCTGAAAACAGAGCCCACCCAGAGAATGACCATCACCGAGTTTATG
AACCACCCTTGGATCATGCAATCAACAAAGGTCCCTCAAACCCCACTGCACACCAGCCGG
GTCCTGAAGGAGGACAAGGAGCGGTGGGAGGATGTCAAGGGGTGTCTTCATGACAAGAAC
AGCGACCAGGCCACTTGGCTGACCAGGTTGTGA
GenBank Gene IDU12779
GeneCard IDNot Available
GenAtlas IDMAPKAPK2
HGNC IDHGNC:6887
Chromosome Location1
Locus1q32
References
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