Glutamate--cysteine ligase catalytic subunit

NameGlutamate--cysteine ligase catalytic subunit
Synonyms
  • 6.3.2.2
  • Gamma-ECS
  • Gamma-glutamylcysteine synthetase
  • GCS heavy chain
  • GLCL
  • GLCLC
Gene NameGCLC
OrganismHuman
Amino acid sequence
>lcl|BSEQ0010186|Glutamate--cysteine ligase catalytic subunit
MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDH
ENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTV
EANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAI
NKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDA
MGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVI
SASVDDRTREERGLEPLKNNNYRISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQ
EGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDI
GWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVL
QGMFYFRKDICKGGNAVVDGCGKAQNSTELAAEEYTLMSIDTIINGKEGVFPGLIPILNS
YLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYS
LILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN
Number of residues637
Molecular Weight72765.14
Theoretical pI5.98
GO Classification
Functions
  • coenzyme binding
  • ADP binding
  • ATP binding
  • glutamate-cysteine ligase activity
  • magnesium ion binding
  • glutamate binding
Processes
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process
  • negative regulation of extrinsic apoptotic signaling pathway
  • regulation of blood vessel size
  • negative regulation of neuron apoptotic process
  • regulation of mitochondrial depolarization
  • response to hormone
  • response to nitrosative stress
  • response to heat
  • response to arsenic-containing substance
  • response to oxidative stress
  • negative regulation of transcription, DNA-templated
  • cell redox homeostasis
  • cellular nitrogen compound metabolic process
  • apoptotic mitochondrial changes
  • small molecule metabolic process
  • cysteine metabolic process
  • glutamate metabolic process
  • negative regulation of apoptotic process
  • glutathione biosynthetic process
  • xenobiotic metabolic process
  • glutathione derivative biosynthetic process
  • sulfur amino acid metabolic process
  • negative regulation of protein ubiquitination
  • L-ascorbic acid metabolic process
Components
  • glutamate-cysteine ligase complex
  • cytosol
General FunctionMagnesium ion binding
Specific FunctionNot Available
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID183039
UniProtKB IDP48506
UniProtKB Entry NameGSH1_HUMAN
Cellular LocationCytoplasmic
Gene sequence
>lcl|BSEQ0010187|Glutamate--cysteine ligase catalytic subunit (GCLC)
ATGGGGCTGCTGTCCCAGGGCTCGCCGCTGAGCTGGGAGGAAACCAAGCGCCATGCCGAC
CACGTGCGGCGGCACGGGATCCTCCAGTTCCTGCACATCTACCACGCCGTCAAGGACCGG
CACAAGGACGTTCTCAAGTGGGGCGATGAGGTGGAATACATGTTGGTATCTTTTGATCAT
GAAAATAAAAAAGTCCGGTTGGTCCTGTCTGGGGAGAAAGTTCTTGAAACTCTGCAAGAG
AAGGGGGAAAGGACAAACCCAAACCATCCTACCCTTTGGAGACCAGAGTATGGGAGTTAC
ATGATTGAAGGGACACCAGGACAGCCCTACGGAGGAACAATGTCCGAGTTCAATACAGTT
GAGGCCAACATGCGAAAACGCCGGAAGGAGGCTACTTCTATATTAGAAGAAAATCAGGCT
CTTTGCACAATAACTTCATTTCCCAGTACCTTAACAAGAAATATCCGACATAGGAGAGGA
GAAAAGGTTGTCATCAATGTACCAATATTTAAGGACAAGAATACACCATCTCCATTTATA
GAAACATTTACTGAGGATGATGAAGCTTCAAGGGCTTCTAAGCCGGATCATATTTACATG
GATGCCATGGGATTTGGAATGGGCAATTGCTGTCTCCAGGTGACATTCCAAGCCTGCAGT
ATATCTGAGGCCAGATACCTTTATGATCAGTTGGCTACTATCTGTCCAATTGTTATGGCT
TTGAGTGCTGCATCTCCCTTTTACCGAGGCTATGTGTCAGACATTGATTGTCGCTGGGGA
GTGATTTCTGCATCTGTAGATGATAGAACTCGGGAGGAGCGAGGACTGGAGCCATTGAAG
AACAATAACTATAGGATCAGTAAATCCCGATATGACTCAATAGACAGCTATTTATCTAAG
TGTGGTGAGAAATATAATGACATCGACTTGACGATAGATAAAGAGATCTACGAACAGCTG
TTGCAGGAAGGCATTGATCATCTCCTGGCCCAGCATGTTGCTCATCTCTTTATTAGAGAC
CCACTGACACTGTTTGAAGAGAAAATACACCTGGATGATGCTAATGAGTCTGACCATTTT
GAGAATATTCAGTCCACAAATTGGCAGACAATGAGATTTAAGCCCCCTCCTCCAAACTCA
GACATTGGATGGAGAGTAGAATTTCGACCCATGGAGGTGCAATTAACAGACTTTGAGAAC
TCTGCCTATGTGGTGTTTGTGGTACTGCTCACCAGAGTGATCCTTTCCTACAAATTGGAT
TTTCTCATTCCACTGTCAAAGGTTGATGAGAACATGAAGGTAGCACAGAAAAGAGATGCT
GTCTTGCAGGGAATGTTTTATTTCAGGAAAGATATTTGCAAAGGTGGCAATGCAGTGGTG
GATGGTTGTGGCAAGGCCCAGAACAGCACGGAGCTCGCTGCAGAGGAGTACACCCTCATG
AGCATAGACACCATCATCAATGGGAAGGAAGGTGTGTTTCCTGGACTGATCCCAATTCTG
AACTCTTACCTTGAAAACATGGAAGTGGATGTGGACACCAGATGTAGTATTCTGAACTAC
CTAAAGCTAATTAAGAAGAGAGCATCTGGAGAACTAATGACAGTTGCCAGATGGATGAGG
GAGTTTATCGCAAACCATCCTGACTACAAGCAAGACAGTGTCATAACTGATGAAATGAAT
TATAGCCTTATTTTGAAGTGTAACCAAATTGCAAATGAATTATGTGAATGCCCAGAGTTA
CTTGGATCAGCATTTAGGAAAGTAAAATATAGTGGAAGTAAAACTGACTCATCCAACTAG
GenBank Gene IDM90656
GeneCard IDNot Available
GenAtlas IDGCLC
HGNC IDHGNC:4311
Chromosome Location6
Locus6p12
References
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  4. Mulcahy RT, Gipp JJ: Identification of a putative antioxidant response element in the 5'-flanking region of the human gamma-glutamylcysteine synthetase heavy subunit gene. Biochem Biophys Res Commun. 1995 Apr 6;209(1):227-33. 7726839
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