Cystathionine gamma-lyase

NameCystathionine gamma-lyase
Synonyms
  • 4.4.1.1
  • Cysteine-protein sulfhydrase
  • Gamma-cystathionase
Gene NameCTH
OrganismHuman
Amino acid sequence
>lcl|BSEQ0001578|Cystathionine gamma-lyase
MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEY
SRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRY
FRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHG
DIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGHSDVVMGLVSVNCESLHNRLRFLQ
NSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQH
ELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVL
KNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS
Number of residues405
Molecular Weight44507.64
Theoretical pI6.69
GO Classification
Functions
  • pyridoxal phosphate binding
  • cystathionine gamma-lyase activity
  • homocysteine desulfhydrase activity
  • L-cysteine desulfhydrase activity
  • carbon-sulfur lyase activity
  • L-cystine L-cysteine-lyase (deaminating)
Processes
  • positive regulation of I-kappaB kinase/NF-kappaB signaling
  • cellular nitrogen compound metabolic process
  • negative regulation of apoptotic signaling pathway
  • small molecule metabolic process
  • positive regulation of NF-kappaB transcription factor activity
  • protein sulfhydration
  • protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
  • protein homotetramerization
  • sulfur amino acid catabolic process
  • sulfur amino acid metabolic process
  • endoplasmic reticulum unfolded protein response
  • cysteine biosynthetic process
  • cysteine biosynthetic process via cystathionine
  • hydrogen sulfide biosynthetic process
  • transsulfuration
  • cysteine metabolic process
Components
  • cytoplasm
  • nucleus
  • cytosol
  • extracellular exosome
General FunctionPyridoxal phosphate binding
Specific FunctionCatalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID262476
UniProtKB IDP32929
UniProtKB Entry NameCGL_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0010559|Cystathionine gamma-lyase (CTH)
ATGCAGGAAAAAGACGCCTCCTCACAAGGTTTCCTGCCACACTTCCAACATTTCGCCACG
CAGGCGATCCATGTGGGCCAGGATCCAGAGCAATGGACCTCCAGGGCTGTAGTGCCCCCC
ATCTCACTGTCCACCACGTTCAAGCAAGGGGCGCCTGGCCAGCACTCGGGTTTTGAATAT
AGCCGTTCTGGAAATCCCACTAGGAATTGCCTTGAAAAAGCAGTGGCAGCACTGGATGGG
GCTAAGTACTGTACAAACAGGTACTTCAGGCAAGTGGCATCTGAATTTGGATTAAAGATT
TCTTTTGTTGATTGTTCCAAAATCAAATTACTAGAGGCAGCAATTACACCAGAAACCAAG
CTTGTTTGGATCGAAACCCCCACAAACCCCACCCAGAAGGTGATTGACATTGAAGGCTGT
GCACATATTGTCCATAAGCATGGAGACATTATTTTGGTCGTGGATAACACTTTTATGTCA
CCATATTTCCAGCGCCCTTTGGCTCTGGGAGCTGATATTTCTATGTATTCTGCAACAAAA
TACATGAATGGCCACAGTGATGTTGTAATGGGCCTGGTGTCTGTTAATTGTGAAAGCCTT
CATAATAGACTTCGTTTCTTGCAAAACTCTCTTGGAGCAGTTCCATCTCCTATTGATTGT
TACCTCTGCAATCGAGGTCTGAAGACTCTACATGTCCGAATGGAAAAGCATTTCAAAAAC
GGAATGGCAGTTGCCCAGTTCCTGGAATCTAATCCTTGGGTAGAAAAGGTTATTTATCCT
GGGCTGCCCTCTCATCCACAGCATGAGTTGGTGAAGCGTCAGTGTACAGGTTGTACAGGG
ATGGTCACCTTTTATATTAAGGGCACTCTTCAGCATGCTGAGATTTTCCTCAAGAACCTA
AAGCTATTTACTCTGGCCGAGAGCTTGGGAGGATTCGAAAGCCTTGCTGAGCTTCCGGCA
ATCATGACTCATGCATCAGTTCTTAAGAATGACAGAGATGTCCTTGGAATTAGTGACACA
CTGATTCGACTTTCTGTGGGCTTAGAGGATGAGGAAGACCTACTGGAAGATCTAGATCAA
GCTTTGAAGGCAGCACACCCTCCAAGTGGAAGTCACAGCTAG
GenBank Gene IDS52784
GeneCard IDNot Available
GenAtlas IDCTH
HGNC IDHGNC:2501
Chromosome Location1
Locus1p31.1
References
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