NameSerine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
Synonyms
  • PP2A subunit A isoform PR65-beta
  • PP2A subunit A isoform R1-beta
Gene NamePPP2R1B
OrganismHuman
Amino acid sequence
>lcl|BSEQ0009188|Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
MAGASELGTGPGAAGGDGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTR
SELLPFLTDTIYDEDEVLLALAEQLGNFTGLVGGPDFAHCLLPPLENLATVEETVVRDKA
VESLRQISQEHTPVALEAYFVPLVKRLASGDWFTSRTSACGLFSVCYPRASNAVKAEIRQ
QFRSLCSDDTPMVRRAAASKLGEFAKVLELDSVKSEIVPLFTSLASDEQDSVRLLAVEAC
VSIAQLLSQDDLETLVMPTLRQAAEDKSWRVRYMVADRFSELQKAMGPKITLNDLIPAFQ
NLLKDCEAEVRAAAAHKVKELGENLPIEDRETIIMNQILPYIKELVSDTNQHVKSALASV
IMGLSTILGKENTIEHLLPLFLAQLKDECPDVRLNIISNLDCVNEVIGIRQLSQSLLPAI
VELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMK
LVQKFGTEWAQNTIVPKVLVMANDPNYLHRMTTLFCINALSEACGQEITTKQMLPIVLKM
AGDQVANVRFNVAKSLQKIGPILDTNALQGEVKPVLQKLGQDEDMDVKYFAQEAISVLAL
A
Number of residues601
Molecular Weight66212.77
Theoretical pINot Available
GO Classification
Functions
  • protein phosphatase type 2A regulator activity
Processes
  • positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
  • protein complex assembly
  • apoptotic process involved in morphogenesis
Components
  • extracellular exosome
  • membrane raft
General FunctionProtein phosphatase type 2a regulator activity
Specific FunctionThe PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDP30154
UniProtKB Entry Name2AAB_HUMAN
Cellular LocationNot Available
Gene sequence
>lcl|BSEQ0013660|Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform (PPP2R1B)
ATGGCGGGCGCATCAGAGCTCGGGACCGGCCCAGGAGCAGCGGGTGGAGATGGAGATGAT
TCGCTATACCCGATCGCGGTTTTAATCGACGAGCTCCGCAATGAAGACGTGCAGCTCCGA
CTCAACAGTATTAAGAAGTTATCAACAATTGCCCTAGCACTTGGAGTAGAAAGGACCCGA
AGTGAATTGTTGCCATTTCTTACAGATACAATTTATGATGAAGATGAGGTACTATTAGCT
CTTGCTGAGCAGCTGGGAAATTTCACTGGCCTAGTGGGAGGTCCTGACTTTGCCCACTGT
CTGCTGCCTCCTTTGGAAAATCTGGCAACTGTGGAAGAGACTGTTGTTCGTGACAAGGCT
GTGGAGTCCCTGAGACAGATCTCCCAGGAGCATACTCCTGTTGCTCTGGAAGCTTATTTT
GTACCTCTGGTGAAACGCTTAGCAAGTGGGGATTGGTTCACCTCTCGCACATCTGCATGT
GGTTTGTTCAGCGTTTGCTATCCCAGGGCATCAAATGCTGTTAAAGCAGAAATCAGACAG
CAATTCCGTTCCTTGTGCTCAGATGACACACCAATGGTACGACGTGCTGCTGCTTCCAAA
TTGGGTGAATTTGCAAAAGTTTTGGAATTAGACAGTGTGAAAAGTGAAATTGTTCCACTG
TTCACTAGTCTAGCTTCAGATGAACAGGATTCAGTGCGCCTCCTTGCTGTGGAAGCTTGT
GTCAGTATTGCCCAGTTATTGTCTCAGGATGACCTTGAGACTTTGGTGATGCCTACACTT
CGACAAGCAGCAGAAGATAAATCTTGGCGCGTTCGCTATATGGTGGCTGACAGATTTTCA
GAGCTCCAGAAAGCCATGGGTCCTAAAATCACCCTAAATGACCTCATCCCCGCCTTTCAG
AACCTACTTAAAGACTGTGAAGCTGAAGTCCGGGCAGCTGCTGCCCACAAAGTAAAAGAA
CTTGGTGAGAACTTGCCCATTGAAGATAGAGAGACCATAATTATGAATCAAATTCTGCCT
TATATAAAGTGTCCTGACGTTCGTTTGAATATCATCTCCAATTTGGATTGTGTAAATGAA
GTGATTGGAATCCGTCAGCTCTCTCAGTCTCTCCTTCCTGCCATAGTGGAGCTGGCAGAA
GATGCCAAATGGAGGGTCCGCCTGGCCATCATTGAGTATATGCCGCTGCTGGCAGGCCAG
CTGGGTGTGGAATTCTTTGATGAAAAGCTGAATTCTTTATGTATGGCTTGGCTCGTGGAC
CATGTATACGCCATCCGAGAAGCTGCCACCAACAACCTCATGAAACTAGTTCAGAAGTTT
GGTACAGAGTGGGCCCAAAATACTATTGTTCCCAAAGTGTTAGTAATGGCAAATGATCCT
AATTACTTGCATAGAATGACCACTTTATTCTGCATTAATGCACTGTCTGAGGCCTGTGGT
CAGGAAATAACTACTAAGCAAATGCTGCCCATCGTATTAAAAATGGCAGGAGACCAAGTA
GCAAATGTTCGCTTCAATGTGGCCAAATCTCTACAAAAGATTGGACCAATTCTAGATACC
AATGCTTTACAGGGAGAAGTGAAGCCAGTACTACAGAAGTTAGGTCAAGATGAAGACATG
GATGTCAAATACTTTGCACAGGAAGCTATAAGTGTTCTTGCATTGGCATAA
GenBank Gene IDNot Available
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:9303
Chromosome Location11
LocusNot Available
References
  1. Baysal BE, Farr JE, Goss JR, Devlin B, Richard CW 3rd: Genomic organization and precise physical location of protein phosphatase 2A regulatory subunit A beta isoform gene on chromosome band 11q23. Gene. 1998 Sep 14;217(1-2):107-16. 9795170
  2. Wang SS, Esplin ED, Li JL, Huang L, Gazdar A, Minna J, Evans GA: Alterations of the PPP2R1B gene in human lung and colon cancer. Science. 1998 Oct 9;282(5387):284-7. 9765152
  3. Baysal BE, Willett-Brozick JE, Taschner PE, Dauwerse JG, Devilee P, Devlin B: A high-resolution integrated map spanning the SDHD gene at 11q23: a 1.1-Mb BAC contig, a partial transcript map and 15 new repeat polymorphisms in a tumour-suppressor region. Eur J Hum Genet. 2001 Feb;9(2):121-9. 11313745
  4. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5. Epub 2003 Dec 21. 14702039
  5. Taylor TD, Noguchi H, Totoki Y, Toyoda A, Kuroki Y, Dewar K, Lloyd C, Itoh T, Takeda T, Kim DW, She X, Barlow KF, Bloom T, Bruford E, Chang JL, Cuomo CA, Eichler E, FitzGerald MG, Jaffe DB, LaButti K, Nicol R, Park HS, Seaman C, Sougnez C, Yang X, Zimmer AR, Zody MC, Birren BW, Nusbaum C, Fujiyama A, Hattori M, Rogers J, Lander ES, Sakaki Y: Human chromosome 11 DNA sequence and analysis including novel gene identification. Nature. 2006 Mar 23;440(7083):497-500. 16554811
  6. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. 15489334
  7. Hemmings BA, Adams-Pearson C, Maurer F, Muller P, Goris J, Merlevede W, Hofsteenge J, Stone SR: alpha- and beta-forms of the 65-kDa subunit of protein phosphatase 2A have a similar 39 amino acid repeating structure. Biochemistry. 1990 Apr 3;29(13):3166-73. 2159327
  8. Abraham D, Podar K, Pacher M, Kubicek M, Welzel N, Hemmings BA, Dilworth SM, Mischak H, Kolch W, Baccarini M: Raf-1-associated protein phosphatase 2A as a positive regulator of kinase activation. J Biol Chem. 2000 Jul 21;275(29):22300-4. 10801873
  9. Lubert EJ, Sarge KD: Interaction between protein phosphatase 2A and members of the importin beta superfamily. Biochem Biophys Res Commun. 2003 Apr 11;303(3):908-13. 12670497
  10. Kitajima TS, Sakuno T, Ishiguro K, Iemura S, Natsume T, Kawashima SA, Watanabe Y: Shugoshin collaborates with protein phosphatase 2A to protect cohesin. Nature. 2006 May 4;441(7089):46-52. Epub 2006 Mar 15. 16541025
  11. Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17. 21269460
  12. Van Damme P, Lasa M, Polevoda B, Gazquez C, Elosegui-Artola A, Kim DS, De Juan-Pardo E, Demeyer K, Hole K, Larrea E, Timmerman E, Prieto J, Arnesen T, Sherman F, Gevaert K, Aldabe R: N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12449-54. doi: 10.1073/pnas.1210303109. Epub 2012 Jul 18. 22814378
  13. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. 24275569
  14. Campbell IG, Manolitsas T: Absence of PPP2R1B gene alterations in primary ovarian cancers. Oncogene. 1999 Nov 4;18(46):6367-9. 10597236
  15. Takagi Y, Futamura M, Yamaguchi K, Aoki S, Takahashi T, Saji S: Alterations of the PPP2R1B gene located at 11q23 in human colorectal cancers. Gut. 2000 Aug;47(2):268-71. 10896920
  16. Hemmer S, Wasenius VM, Haglund C, Zhu Y, Knuutila S, Franssila K, Joensuu H: Alterations in the suppressor gene PPP2R1B in parathyroid hyperplasias and adenomas. Cancer Genet Cytogenet. 2002 Apr 1;134(1):13-7. 11996789