NameAlpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Synonyms
  • 2.4.1.101
  • GGNT1
  • GlcNAc-T I
  • GLCT1
  • GLYT1
  • GNT-I
  • MGAT
  • N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Gene NameMGAT1
OrganismHuman
Amino acid sequence
>lcl|BSEQ0020775|Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
MLKKQSAGLVLWGAILFVAWNALLLLFFWTRPAPGRPPSVSALDGDPASLTREVIRLAQD
AEVELERQRGLLQQIGDALSSQRGRVPTAAPPAQPRVPVTPAPAVIPILVIACDRSTVRR
CLDKLLHYRPSAELFPIIVSQDCGHEETAQAIASYGSAVTHIRQPDLSSIAVPPDHRKFQ
GYYKIARHYRWALGQVFRQFRFPAAVVVEDDLEVAPDFFEYFRATYPLLKADPSLWCVSA
WNDNGKEQMVDASRPELLYRTDFFPGLGWLLLAELWAELEPKWPKAFWDDWMRRPEQRQG
RACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQFVHFTQLDLSYLQREAYDRDFLA
RVYGAPQLQVEKVRTNDRKELGEVRVQYTGRDSFKAFAKALGVMDDLKSGVPRAGYRGIV
TFQFRGRRVHLAPPLTWEGYDPSWN
Number of residues445
Molecular Weight50877.88
Theoretical pINot Available
GO Classification
Functions
  • alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity
  • metal ion binding
  • acetylglucosaminyltransferase activity
Processes
  • carbohydrate metabolic process
  • cellular protein metabolic process
  • post-translational protein modification
  • protein N-linked glycosylation via asparagine
  • protein glycosylation
  • UDP-N-acetylglucosamine catabolic process
  • in utero embryonic development
Components
  • extracellular vesicle
  • Golgi membrane
  • membrane
  • integral component of membrane
  • extracellular exosome
General FunctionMetal ion binding
Specific FunctionInitiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
Pfam Domain Function
Transmembrane Regions7-29
GenBank Protein IDNot Available
UniProtKB IDP26572
UniProtKB Entry NameMGAT1_HUMAN
Cellular LocationGolgi apparatus membrane
Gene sequence
>lcl|BSEQ0020776|Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (MGAT1)
ATGCTGAAGAAGCAGTCTGCAGGGCTTGTGCTGTGGGGCGCTATCCTCTTTGTGGCCTGG
AATGCCCTGCTGCTCCTCTTCTTCTGGACGCGCCCAGCACCTGGCAGGCCACCCTCAGTC
AGCGCTCTCGATGGCGACCCCGCCAGCCTCACCCGGGAAGTGATTCGCCTGGCCCAAGAC
GCCGAGGTGGAGCTGGAGCGGCAGCGTGGGCTGCTGCAGCAGATCGGGGATGCCCTGTCG
AGCCAGCGGGGGAGGGTGCCCACCGCGGCCCCTCCCGCCCAGCCGCGTGTGCCTGTGACC
CCCGCGCCGGCGGTGATTCCCATCCTGGTCATCGCCTGTGACCGCAGCACTGTTCGGCGC
TGCCTGGACAAGCTGCTGCATTATCGGCCCTCGGCTGAGCTCTTCCCCATCATCGTTAGC
CAGGACTGCGGGCACGAGGAGACGGCCCAGGCCATCGCCTCCTACGGCAGCGCGGTCACG
CACATCCGGCAGCCCGACCTGAGCAGCATTGCGGTGCCGCCGGACCACCGCAAGTTCCAG
GGCTACTACAAGATCGCGCGCCACTACCGCTGGGCGCTGGGCCAGGTCTTCCGGCAGTTT
CGCTTCCCCGCGGCCGTGGTGGTGGAGGATGACCTGGAGGTGGCCCCGGACTTCTTCGAG
TACTTTCGGGCCACCTATCCGCTGCTGAAGGCCGACCCCTCCCTGTGGTGCGTCTCGGCC
TGGAATGACAACGGCAAGGAGCAGATGGTGGACGCCAGCAGGCCTGAGCTGCTCTACCGC
ACCGACTTTTTCCCTGGCCTGGGCTGGCTGCTGTTGGCCGAGCTCTGGGCTGAGCTGGAG
CCCAAGTGGCCAAAGGCCTTCTGGGACGACTGGATGCGGCGGCCGGAGCAGCGGCAGGGG
CGGGCCTGCATACGCCCTGAGATCTCAAGAACGATGACCTTTGGCCGCAAGGGTGTGAGC
CACGGGCAGTTCTTTGACCAGCACCTCAAGTTTATCAAGCTGAACCAGCAGTTTGTGCAC
TTCACCCAGCTGGACCTGTCTTACCTGCAGCGGGAGGCCTATGACCGAGATTTCCTCGCC
CGCGTCTACGGTGCTCCCCAGCTGCAGGTGGAGAAAGTGAGGACCAATGACCGGAAGGAG
CTGGGGGAGGTGCGGGTGCAGTATACGGGCAGGGACAGCTTCAAGGCTTTCGCCAAGGCT
CTGGGTGTCATGGATGACCTTAAGTCGGGGGTTCCGAGAGCTGGCTACCGGGGTATTGTC
ACCTTCCAGTTCCGGGGCCGCCGTGTCCACCTGGCGCCCCCACTGACGTGGGAGGGCTAT
GATCCTAGCTGGAATTAG
GenBank Gene IDNot Available
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:7044
Chromosome Location5
LocusNot Available
References
  1. Kumar R, Yang J, Larsen RD, Stanley P: Cloning and expression of N-acetylglucosaminyltransferase I, the medial Golgi transferase that initiates complex N-linked carbohydrate formation. Proc Natl Acad Sci U S A. 1990 Dec;87(24):9948-52. 1702225
  2. Hull E, Sarkar M, Spruijt MP, Hoppener JW, Dunn R, Schachter H: Organization and localization to chromosome 5 of the human UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I gene. Biochem Biophys Res Commun. 1991 Apr 30;176(2):608-15. 1827260
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