Arachidonate 5-lipoxygenase-activating protein

NameArachidonate 5-lipoxygenase-activating protein
Synonyms
  • FLAP
  • MK-886-binding protein
Gene NameALOX5AP
OrganismHuman
Amino acid sequence
>lcl|BSEQ0011708|Arachidonate 5-lipoxygenase-activating protein
MDQETVGNVVLLAIVTLISVVQNGFFAHKVEHESRTQNGRSFQRTGTLAFERVYTANQNC
VDAYPTFLAVLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGYLGERTQSTPGYIFGKRIIL
FLFLMSVAGIFNYYLIFFFGSDFENYIKTISTTISPLLLIP
Number of residues161
Molecular Weight18156.96
Theoretical pI8.64
GO Classification
Functions
  • protein N-terminus binding
  • arachidonic acid binding
  • enzyme activator activity
Processes
  • cellular response to calcium ion
  • arachidonic acid metabolic process
  • leukotriene metabolic process
  • lipoxin metabolic process
  • lipoxygenase pathway
  • protein homotrimerization
  • small molecule metabolic process
  • leukotriene biosynthetic process
Components
  • nuclear envelope
  • membrane
  • endoplasmic reticulum
  • endoplasmic reticulum membrane
  • integral component of membrane
  • nuclear membrane
General FunctionProtein n-terminus binding
Specific FunctionRequired for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.
Pfam Domain Function
Transmembrane Regions9-30 53-77 81-102 116-128
GenBank Protein IDNot Available
UniProtKB IDP20292
UniProtKB Entry NameAL5AP_HUMAN
Cellular LocationNucleus membrane
Gene sequence
>lcl|BSEQ0011709|Arachidonate 5-lipoxygenase-activating protein (ALOX5AP)
ATGCTCACATTTAATCACGATGCTCCCTGGCATACACAGAAGACTCTGAAAACTTCTGAA
TTTGGGAAATCCTTTGGCACCTTGGGGCACATTGGGAACATAAGCCATCAGTGCTGGGCA
GGTTGTGCAGCTGGAGGCAGAGCAGTCCTCTCTGGGGAGCCTGAAGCAAACATGGATCAA
GAAACTGTAGGCAATGTTGTCCTGTTGGCCATCGTCACCCTCATCAGCGTGGTCCAGAAT
GGATTCTTTGCCCATAAAGTGGAGCACGAAAGCAGGACCCAGAATGGGAGGAGCTTCCAG
AGGACCGGAACACTTGCCTTTGAGCGGGTCTACACTGCCAACCAGAACTGTGTAGATGCG
TACCCCACTTTCCTCGCTGTGCTCTGGTCTGCGGGGCTACTTTGCAGCCAAGTTCCTGCT
GCGTTTGCTGGACTGATGTACTTGTTTGTGAGGCAAAAGTACTTTGTCGGTTACCTAGGA
GAGAGAACGCAGAGCACCCCTGGCTACATATTTGGGAAACGCATCATACTCTTCCTGTTC
CTCATGTCCGTTGCTGGCATATTCAACTATTACCTCATCTTCTTTTTCGGAAGTGACTTT
GAAAACTACATAAAGACGATCTCCACCACCATCTCCCCTCTACTTCTCATTCCCTAA
GenBank Gene IDX52195
GeneCard IDNot Available
GenAtlas IDALOX5AP
HGNC IDHGNC:436
Chromosome Location13
Locus13q12
References
  1. Dixon RA, Diehl RE, Opas E, Rands E, Vickers PJ, Evans JF, Gillard JW, Miller DK: Requirement of a 5-lipoxygenase-activating protein for leukotriene synthesis. Nature. 1990 Jan 18;343(6255):282-4. 2300173
  2. Kennedy BP, Diehl RE, Boie Y, Adam M, Dixon RA: Gene characterization and promoter analysis of the human 5-lipoxygenase-activating protein (FLAP). J Biol Chem. 1991 May 5;266(13):8511-6. 1673682
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  5. Mancini JA, Abramovitz M, Cox ME, Wong E, Charleson S, Perrier H, Wang Z, Prasit P, Vickers PJ: 5-lipoxygenase-activating protein is an arachidonate binding protein. FEBS Lett. 1993 Mar 8;318(3):277-81. 8440384
  6. Woods JW, Evans JF, Ethier D, Scott S, Vickers PJ, Hearn L, Heibein JA, Charleson S, Singer II: 5-lipoxygenase and 5-lipoxygenase-activating protein are localized in the nuclear envelope of activated human leukocytes. J Exp Med. 1993 Dec 1;178(6):1935-46. 8245774
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