Transcobalamin-2

NameTranscobalamin-2
Synonyms
  • TC II
  • TC-2
  • TC2
  • TCII
  • Transcobalamin II
Gene NameTCN2
OrganismHuman
Amino acid sequence
>lcl|BSEQ0037000|Transcobalamin-2
MRHLGAFLFLLGVLGALTEMCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRL
SSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSMGQLALYLLALRANCEFVR
GHKGDRLVSQLKWFLEDEKRAIGHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDKLL
YAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVREEILKAQTPEGHF
GNVYSTPLALQFLMTSPMRGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKT
YIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLK
KAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGETI
ELRLVSW
Number of residues427
Molecular Weight47534.54
Theoretical pI6.89
GO Classification
Functions
  • cobalamin binding
  • metal ion binding
Processes
  • cobalamin metabolic process
  • cobalt ion transport
  • cobalamin transport
  • small molecule metabolic process
  • vitamin metabolic process
  • water-soluble vitamin metabolic process
Components
  • extracellular region
  • extracellular space
  • extracellular exosome
  • endosome
  • lysosomal lumen
General FunctionMetal ion binding
Specific FunctionPrimary vitamin B12-binding and transport protein. Delivers cobalamin to cells.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID339196
UniProtKB IDP20062
UniProtKB Entry NameTCO2_HUMAN
Cellular LocationSecreted
Gene sequence
>lcl|BSEQ0010261|Transcobalamin-2 (TCN2)
ATGAGGCACCTTGGGGCCTTCCTCTTCCTTCTGGGGGTCCTGGGGGCCCTCACTGAGATG
TGTGAAATACCAGAGATGGACAGCCATCTGGTAGAGAAGTTGGGCCAGCACCTCTTACCT
TGGATGGACCGGCTTTCCCTGGAGCACTTGAACCCCAGCATCTATGTGGGCCTACGCCTC
TCCAGTCTGCAGGCTGGGACCAAGGAAGACCTCTACCTGCACAGCCTCAAGCTTGGTTAC
CAGCAGTGCCTCCTAGGGTCTGCCTTCAGCGAGGATGACGGTGACTGCCAGGGCAAGCCT
TCCATGGGCCAGCTGGCCCTCTACCTGCTCGCTCTCAGAGCCAACTGTGAGTTTGTCAGG
GGCCACAAGGGGGACAGGCTGGTCTCACAGCTCAAATGGTTCCTGGAGGATGAGAAGAGA
GCCATTGGGCATGATCACAAGGGCCACCCCCACACTAGCTACTACCAGTATGGCCTGGGC
ATTCTGGCCCTGTGTCTCCACCAGAAGCGGGTCCATGACAGCGTGGTGGACAAACTTCTG
TATGCTGTGGAACCTTTCCACCAGGGCCACCATTCTGTGGACACAGCAGCCATGGCAGGC
TTGGCATTCACCTGTCTGAAGCGCTCAAACTTCAACCCTGGTCGGAGACAACGGATCACC
ATGGCCATCAGAACAGTGCGAGAGGAGATCTTGAAGGCCCAGACCCCCGAGGGCCACTTT
GGGAATGTCTACAGCACCCCATTGGCATTACAGTTCCTCATGACTTCCCCCATGCGTGGG
GCAGAACTGGGAACAGCATGTCTCAAGGCGAGGGTTGCTTTGCTGGCCAGTCTGCAGGAT
GGAGCCTTCCAGAATGCTCTCATGATTTCCCAGCTGCTGCCCGTTCTGAACCACAAGACC
TACATTGATCTGATCTTCCCAGACTGTCTGGCACCACGAGTCATGTTGGAACCAGCTGCT
GAGACCATTCCTCAGACCCAAGAGATCATCAGTGTCACGCTGCAGGTGCTTAGTCTCTTG
CCGCCGTACAGACAGTCCATCTCTGTTCTGGCCGGGTCCACCGTGGAAGATGTCCTGAAG
AAGGCCCATGAGTTAGGAGGATTCACATATGAAACACAGGCCTCCTTGTCAGGCCCCTAC
TTAACCTCCGTGATGGGGAAAGCGGCCGGAGAAAGGGAGTTCTGGCAGCTTCTCCGAGAC
CCCAACACCCCACTGTTGCAAGGTATTGCTGACTACAGACCCAAGGATGGAGAAACCATT
GAGCTGAGGCTGGTTAGCTGGTAG
GenBank Gene IDM60396
GeneCard IDNot Available
GenAtlas IDTCN2
HGNC IDHGNC:11653
Chromosome Location22
Locus22q12.2
References
  1. Platica O, Janeczko R, Quadros EV, Regec A, Romain R, Rothenberg SP: The cDNA sequence and the deduced amino acid sequence of human transcobalamin II show homology with rat intrinsic factor and human transcobalamin I. J Biol Chem. 1991 Apr 25;266(12):7860-3. 1708393
  2. Li N, Seetharam S, Lindemans J, Alpers DH, Arwert F, Seetharam B: Isolation and sequence analysis of variant forms of human transcobalamin II. Biochim Biophys Acta. 1993 Feb 20;1172(1-2):21-30. 8439564
  3. Regec A, Quadros EV, Platica O, Rothenberg SP: The cloning and characterization of the human transcobalamin II gene. Blood. 1995 May 15;85(10):2711-9. 7742531
  4. Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I: A genome annotation-driven approach to cloning the human ORFeome. Genome Biol. 2004;5(10):R84. Epub 2004 Sep 30. 15461802
  5. Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP, et al.: The DNA sequence of human chromosome 22. Nature. 1999 Dec 2;402(6761):489-95. 10591208
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  7. Quadros EV, Rothenberg SP, Pan YC, Stein S: Purification and molecular characterization of human transcobalamin II. J Biol Chem. 1986 Nov 25;261(33):15455-60. 3782074
  8. Quadros EV, Sai P, Rothenberg SP: Functional human transcobalamin II isoproteins are secreted by insect cells using the baculovirus expression system. Blood. 1993 Mar 1;81(5):1239-45. 8443384
  9. Wuerges J, Garau G, Geremia S, Fedosov SN, Petersen TE, Randaccio L: Structural basis for mammalian vitamin B12 transport by transcobalamin. Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4386-91. Epub 2006 Mar 14. 16537422
  10. Namour F, Olivier J, Abdelmouttaleb I, Adjalla C, Debard R, Salvat C, Gueant J: Transcobalamin codon 259 polymorphism in HT-29 and Caco-2 cells and in Caucasians: relation to transcobalamin and homocysteine concentration in blood. Blood. 2001 Feb 15;97(4):1092-8. 11159542