cAMP-dependent protein kinase catalytic subunit alpha

NamecAMP-dependent protein kinase catalytic subunit alpha
Synonyms
  • 2.7.11.11
  • PKA C-alpha
  • PKACA
Gene NamePRKACA
OrganismHuman
Amino acid sequence
>lcl|BSEQ0007218|cAMP-dependent protein kinase catalytic subunit alpha
MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVML
VKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMV
MEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGY
IQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFF
ADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFAT
TDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF
Number of residues351
Molecular Weight40589.38
Theoretical pI9.22
GO Classification
Functions
  • protein serine/threonine/tyrosine kinase activity
  • cAMP-dependent protein kinase activity
  • ATP binding
  • protein kinase A regulatory subunit binding
  • protein kinase binding
  • ubiquitin protein ligase binding
Processes
  • negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning
  • activation of protein kinase A activity
  • calcium-mediated signaling using intracellular calcium source
  • G2/M transition of mitotic cell cycle
  • positive regulation of cell cycle arrest
  • triglyceride catabolic process
  • mitotic cell cycle
  • epidermal growth factor receptor signaling pathway
  • innate immune response
  • regulation of cardiac muscle contraction
  • organelle organization
  • transmembrane transport
  • signal transduction
  • regulation of cellular respiration
  • fibroblast growth factor receptor signaling pathway
  • activation of phospholipase C activity
  • neurotrophin TRK receptor signaling pathway
  • regulation of ryanodine-sensitive calcium-release channel activity
  • blood coagulation
  • regulation of protein processing
  • regulation of heart rate
  • protein phosphorylation
  • cellular response to glucose stimulus
  • regulation of proteasomal protein catabolic process
  • protein autophosphorylation
  • stimulatory C-type lectin receptor signaling pathway
  • sperm capacitation
  • gluconeogenesis
  • energy reserve metabolic process
  • regulation of osteoblast differentiation
  • regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
  • mesoderm formation
  • regulation of insulin secretion
  • peptidyl-threonine phosphorylation
  • cytosolic calcium ion homeostasis
  • small molecule metabolic process
  • cellular response to epinephrine stimulus
  • cellular response to glucagon stimulus
  • positive regulation of cell proliferation
  • regulation of protein binding
  • renal water homeostasis
  • intracellular signal transduction
  • negative regulation of meiotic cell cycle
  • water transport
  • positive regulation of protein export from nucleus
  • carbohydrate metabolic process
  • neural tube closure
  • peptidyl-serine phosphorylation
  • cell communication by electrical coupling involved in cardiac conduction
  • glucose metabolic process
  • regulation of synaptic transmission, glutamatergic
  • regulation of bicellular tight junction assembly
  • cellular response to parathyroid hormone stimulus
Components
  • perinuclear region of cytoplasm
  • neuromuscular junction
  • mitochondrion
  • centrosome
  • motile cilium
  • calcium channel complex
  • nucleus
  • plasma membrane raft
  • nucleotide-activated protein kinase complex
  • cAMP-dependent protein kinase complex
  • Golgi apparatus
  • cytosol
  • extracellular exosome
  • neuron projection
  • ciliary base
  • sperm midpiece
  • nucleoplasm
General FunctionUbiquitin protein ligase binding
Specific FunctionPhosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID35479
UniProtKB IDP17612
UniProtKB Entry NameKAPCA_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0017217|cAMP-dependent protein kinase catalytic subunit alpha (PRKACA)
ATGTTCACCTTGGGATACCATGGGGCCGAGGAGTGGGCATCGGGCGAGACTTCCCTTGAA
CCAGCTCTGGAGCAATGCCCTTGCGATTGCCACGGAGAGGAACAGGGCCAGCTGGGGGCC
AAGGTGACAGCTGAGCCCCCCAAGGGCACCTGCTACCCGAGCCCCCGAGGCCTCCATCCC
AGGCCCGGCCTCCCCAGAGAAGGGGCCAGAGGAACAGCCAAATCCCAAGGCTATAAATAC
CTCGGCTGGCGTCGGCCCTGGCCATGGAATGTCGTGAAAGAATTCTTAGCCAAAGCCAAA
GAAGATTTTCTTAAAAAATGGGAAAGTCCCGCTCAGAACACAGCCCACTTGGATCAGTTT
GAACGAATCAAGACCCTCGGCACGGGCTCCTTCGGGCGGGTGATGCTGGTGAAACACAAG
GAGACCGGGAACCACTATGCCATGAAGATCCTCGACAAACAGAAGGTGGTGAAACTGAAA
CAGATCGAACACACCCTGAATGAAAAGCGCATCCTGCAAGCTGTCAACTTTCCGTTCCTC
GTCAAACTCGAGTTCTCCTTCAAGGACAACTCAAACTTATACATGGTCATGGAGTACGTG
CCCGGCGGGGAGATGTTCTCACACCTACGGCGGATCGGAAGGTTCAGTGAGCCCCATGCC
CGTTTCTACGCGGCCCAGATCGTCCTGACCTTTGAGTATCTGCACTCGCTGGATCTCATC
TACAGGGACCTGAAGCCGGAGAATCTGCTCATTGACCAGCAGGGCTACATTCAGGTGACA
GACTTCGGTTTCGCCAAGCGCGTGAAGGGCCGCACTTGGACCTTGTGCGGCACCCCTGAG
TACCTGGCCCCTGAGATTATCCTGAGCAAAGGCTACAACAAGGCCGTGGACTGGTGGGCC
CTGGGGGTTCTTATCTATGAAATGGCCGCTGGCTACCCGCCCTTCTTCGCAGACCAGCCC
ATCCAGATCTATGAGAAGATCGTCTCTGGGAAGGTGCGCTTCCCTTCCCACTTCAGCTCT
GACTTGAAGGACCTGCTGCGGAACCTCCTGCAGGTAGATCTCACCAAGCGCTTTGGGAAC
CTCAAGAATGGGGTCAACGATATCAAGAACCACAAGTGGTTTGCCACAACTGACTGGATT
GCCATCTACCAGAGGAAGGTGGAAGCTCCCTTCATACCAAAGTTTAAAGGCCCTGGGGAT
ACGAGTAACTTTGACGACTATGAGGAAGAAGAAATCCGGGTCTCCATCAATGAGAAGTGT
GGCAAGGAGTTTTCTGAGTTTTAG
GenBank Gene IDX07767
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:9380
Chromosome Location19
Locus19p13.1
References
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