Tyrosine-protein kinase Lck

NameTyrosine-protein kinase Lck
Synonyms
  • 2.7.10.2
  • Leukocyte C-terminal Src kinase
  • LSK
  • Lymphocyte cell-specific protein-tyrosine kinase
  • p56-LCK
  • Protein YT16
  • Proto-oncogene Lck
  • T cell-specific protein-tyrosine kinase
Gene NameLCK
OrganismHuman
Amino acid sequence
>lcl|BSEQ0037093|Tyrosine-protein kinase Lck
MGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGTLLIRNGSEVRDPLVTYEGSNPPASP
LQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKAN
SLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKH
YKIRNLDNGGFYISPRITFPGLHELVRHYTNASDGLCTRLSRPCQTQKPQKPWWEDEWEV
PRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRL
VRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNY
IHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIK
SDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKER
PEDRPTFDYLRSVLEDFFTATEGQYQPQP
Number of residues509
Molecular Weight58000.15
Theoretical pI5.03
GO Classification
Functions
  • protein C-terminus binding
  • glycoprotein binding
  • protein kinase binding
  • protein tyrosine kinase activity
  • phosphatidylinositol 3-kinase binding
  • ATP binding
  • identical protein binding
  • SH2 domain binding
  • CD4 receptor binding
  • protein serine/threonine phosphatase activity
  • CD8 receptor binding
  • antigen binding
  • non-membrane spanning protein tyrosine kinase activity
  • protein phosphatase binding
  • ATPase binding
Processes
  • positive regulation of uterine smooth muscle contraction
  • positive regulation of tyrosine phosphorylation of Stat5 protein
  • cellular response to peptide hormone stimulus
  • phosphatidylinositol-mediated signaling
  • epidermal growth factor receptor signaling pathway
  • regulation of defense response to virus by virus
  • response to mechanical stimulus
  • innate immune response
  • peptidyl-tyrosine autophosphorylation
  • hemopoiesis
  • positive regulation of gene expression
  • regulation of cell proliferation
  • B cell receptor signaling pathway
  • cellular zinc ion homeostasis
  • response to zinc ion
  • Fc-epsilon receptor signaling pathway
  • T cell differentiation
  • transmembrane receptor protein tyrosine kinase signaling pathway
  • leukocyte migration
  • positive regulation of T cell activation
  • positive regulation of T cell receptor signaling pathway
  • blood coagulation
  • T cell costimulation
  • response to hydrogen peroxide
  • positive regulation of gamma-delta T cell differentiation
  • response to drug
  • T cell receptor signaling pathway
  • fibroblast growth factor receptor signaling pathway
  • protein dephosphorylation
  • neurotrophin TRK receptor signaling pathway
  • regulation of lymphocyte activation
  • aging
  • protein phosphorylation
  • positive regulation of intrinsic apoptotic signaling pathway
  • platelet activation
  • activation of cysteine-type endopeptidase activity involved in apoptotic process
  • viral process
  • release of sequestered calcium ion into cytosol
Components
  • pericentriolar material
  • cell-cell junction
  • immunological synapse
  • plasma membrane
  • membrane raft
  • cytosol
  • endocytic vesicle
  • extracellular exosome
  • extrinsic component of cytoplasmic side of plasma membrane
General FunctionSh2 domain binding
Specific FunctionNon-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID36808
UniProtKB IDP06239
UniProtKB Entry NameLCK_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0020487|Tyrosine-protein kinase Lck (LCK)
ATGGGCTGTGGCTGCAGCTCACACCCGGAAGATGACTGGATGGAAAACATCGATGTGTGT
GAGAACTGCCATTATCCCATAGTCCCACTGGATGGCAAGGGCACGCTGCTCATCCGAAAT
GGCTCTGAGGTGCGGGACCCACTGGTTACCTACGAAGGCTCCAATCCGCCGGCTTCCCCA
CTGCAAGACAACCTGGTTATCGCTCTGCACAGCTATGAGCCCTCTCACGACGGAGATCTG
GGCTTTGAGAAGGGGGAACAGCTCCGCATCCTGGAGCAGAGCGGCGAGTGGTGGAAGGCG
CAGTCCCTGACCACGGGCCAGGAAGGCTTCATCCCCTTCAATTTTGTGGCCAAAGCGAAC
AGCCTGGAGCCCGAACCCTGGTTCTTCAAGAACCTGAGCCGCAAGGACGCGGAGCGGCAG
CTCCTGGCGCCCGGGAACACTCACGGCTCCTTCCTCATCCGGGAGAGCGAGAGCACCGCG
GGATCGTTTTCACTGTCGGTCCGGGACTTCGACCAGAACCAGGGAGAGGTGGTGAAACAT
TACAAGATCCGTAATCTGGACAACGGTGGCTTCTACATCTCCCCTCGAATCACTTTTCCC
GGCCTGCATGAACTGGTCCGCCATTACACCAATGCTTCAGATGGGCTGTGCACACGGTTG
AGCCGCCCCTGCCAGACCCAGAAGCCCCAGAAGCCGTGGTGGGAGGACGAGTGGGAGGTT
CCCAGGGAGACGCTGAAGCTGGTGGAGCGGCTGGGGGCTGGACAGTTCGGGGAGGTGTGG
ATGGGGTACTACAACGGGCACACGAAGGTGGCGGTGAAGAGCCTGAAGCAGGGCAGCATG
TCCCCGGACGCCTTCCTGGCCGAGGCCAACCTCATGAAGCAGCTGCAACACCAGCGGCTG
GTTCGGCTCTACGCTGTGGTCACCCAGGAGCCCATCTACATCATCACTGAATACATGGAG
AATGGGAGTCTAGTGGATTTTCTCAAGACCCCTTCAGGCATCAAGTTGACCATCAACAAA
CTCCTGGACATGGCAGCCCAAATTGCAGAAGGCATGGCATTCATTGAAGAGCGGAATTAT
ATTCATCGTGACCTTCGGGCTGCCAACATTCTGGTGTCTGACACCCTGAGCTGCAAGATT
GCAGACTTTGGCCTAGCACGCCTCATTGAGGACAACGAGTACACAGCCAGGGAGGGGGCC
AAGTTTCCCATTAAGTGGACAGCGCCAGAAGCCATTAACTACGGGACATTCACCATCAAG
TCAGATGTGTGGTCTTTTGGGATCCTGCTGACGGAAATTGTCACCCACGGCCGCATCCCT
TACCCAGGGATGACCAACCCGGAGGTGATTCAGAACCTGGAGCGAGGCTACCGCATGGTG
CGCCCTGACAACTGTCCAGAGGAGCTGTACCAACTCATGAGGCTGTGCTGGAAGGAGCGC
CCAGAGGACCGGCCCACCTTTGACTACCTGCGCAGTGTGCTGGAGGACTTCTTCACGGCC
ACAGAGGGCCAGTACCAGCCTCAGCCTTGA
GenBank Gene IDX05027
GeneCard IDNot Available
GenAtlas IDLCK
HGNC IDHGNC:6524
Chromosome Location1
Locus1p34.3
References
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