Glyceraldehyde-3-phosphate dehydrogenase

NameGlyceraldehyde-3-phosphate dehydrogenase
Synonyms
  • 1.2.1.12
  • GAPD
  • GAPDH
  • Peptidyl-cysteine S-nitrosylase GAPDH
Gene NameGAPDH
OrganismHuman
Amino acid sequence
>lcl|BSEQ0037043|Glyceraldehyde-3-phosphate dehydrogenase
MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTV
KAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVI
ISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHA
ITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANV
SVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAG
IALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Number of residues335
Molecular Weight36053.0
Theoretical pI8.73
GO Classification
Functions
  • peptidyl-cysteine S-nitrosylase activity
  • NADP binding
  • identical protein binding
  • glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
  • NAD binding
  • microtubule binding
Processes
  • carbohydrate metabolic process
  • peptidyl-cysteine S-trans-nitrosylation
  • glucose metabolic process
  • neuron apoptotic process
  • canonical glycolysis
  • negative regulation of translation
  • protein stabilization
  • gluconeogenesis
  • cellular response to interferon-gamma
  • microtubule cytoskeleton organization
  • small molecule metabolic process
Components
  • nuclear membrane
  • perinuclear region of cytoplasm
  • cytoplasm
  • intracellular membrane-bounded organelle
  • membrane
  • lipid particle
  • ribonucleoprotein complex
  • microtubule cytoskeleton
  • nucleus
  • plasma membrane
  • vesicle
  • cytosol
  • GAIT complex
  • extracellular exosome
General FunctionPeptidyl-cysteine s-nitrosylase activity
Specific FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID31645
UniProtKB IDP04406
UniProtKB Entry NameG3P_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0018970|Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
ATGGTTTACATGTTCCAATATGATTCCACCCATGGCAAATTCCATGGCACCGTCAAGGCT
GAGAACGGGAAGCTTGTCATCAATGGAAATCCCATCACCATCTTCCAGGAGCGAGATCCC
TCCAAAATCAAGTGGGGCGATGCTGGCGCTGAGTACGTCGTGGAGTCCACTGGCGTCTTC
ACCACCATGGAGAAGGCTGGGGCTCATTTGCAGGGGGGAGCCAAAAGGGTCATCATCTCT
GCCCCCTCTGCTGATGCCCCCATGTTCGTCATGGGTGTGAACCATGAGAAGTATGACAAC
AGCCTCAAGATCATCAGCAATGCCTCCTGCACCACCAACTGCTTAGCACCCCTGGCCAAG
GTCATCCATGACAACTTTGGTATCGTGGAAGGACTCATGACCACAGTCCATGCCATCACT
GCCACCCAGAAGACTGTGGATGGCCCCTCCGGGAAACTGTGGCGTGATGGCCGCGGGGCT
CTCCAGAACATCATCCCTGCCTCTACTGGCGCTGCCAAGGCTGTGGGCAAGGTCATCCCT
GAGCTGAACGGGAAGCTCACTGGCATGGCCTTCCGTGTCCCCACTGCCAACGTGTCAGTG
GTGGACCTGACCTGCCGTCTAGAAAAACCTGCCAAATATGATGACATCAAGAAGGTGGTG
AAGCAGGCGTCGGAGGGCCCCCTCAAGGGCATCCTGGGCTACACTGAGCACCAGGTGGTC
TCCTCTGACTTCAACAGCGACACCCACTCCTCCACCTTTGACGCTGGGGCTGGCATTGCC
CTCAACGACCACTTTGTCAAGCTCATTTCCTGGTATGACAACGAATTTGGCTACAGCAAC
AGGGTGGTGGACCTCATGGCCCACATGGCCTCCAAGGAGTAA
GenBank Gene IDX01677
GeneCard IDNot Available
GenAtlas IDGAPDH
HGNC IDHGNC:4141
Chromosome Location12
Locus12p13
References
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