Dihydrofolate reductase

NameDihydrofolate reductase
Synonyms
  • 1.5.1.3
Gene NameDHFR
OrganismHuman
Amino acid sequence
>lcl|BSEQ0036993|Dihydrofolate reductase
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFS
IPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSS
VYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKF
EVYEKND
Number of residues187
Molecular Weight21452.61
Theoretical pI7.6
GO Classification
Functions
  • methotrexate binding
  • drug binding
  • dihydrofolate reductase activity
  • folic acid binding
  • mRNA binding
  • NADPH binding
Processes
  • tetrahydrobiopterin biosynthetic process
  • water-soluble vitamin metabolic process
  • glycine biosynthetic process
  • mitotic cell cycle
  • regulation of removal of superoxide radicals
  • positive regulation of nitric-oxide synthase activity
  • nucleotide biosynthetic process
  • response to methotrexate
  • dihydrofolate metabolic process
  • G1/S transition of mitotic cell cycle
  • one-carbon metabolic process
  • folic acid metabolic process
  • tetrahydrofolate biosynthetic process
  • nitric oxide metabolic process
  • regulation of nitric-oxide synthase activity
  • small molecule metabolic process
  • tetrahydrofolate metabolic process
  • regulation of transcription involved in G1/S transition of mitotic cell cycle
  • oxidation-reduction process
  • axon regeneration
  • vitamin metabolic process
Components
  • cytosol
  • nucleoplasm
General FunctionNadph binding
Specific FunctionKey enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID182724
UniProtKB IDP00374
UniProtKB Entry NameDYR_HUMAN
Cellular LocationNot Available
Gene sequence
>lcl|BSEQ0016067|Dihydrofolate reductase (DHFR)
ATGGTTGGTTCGCTAAACTGCATCGTCGCTGTGTCCCAGAACATGGGCATCGGCAAGAAC
GGGGACCTGCCCTGGCCACCGCTCAGGAATGAATTCAGATATTTCCAGAGAATGACCACA
ACCTCTTCAGTAGAAGGTAAACAGAATCTGGTGATTATGGGTAAGAAGACCTGGTTCTCC
ATTCCTGAGAAGAATCGACCTTTAAAGGGTAGAATTAATTTAGTTCTCAGCAGAGAACTC
AAGGAACCTCCACAAGGAGCTCATTTTCTTTCCAGAAGTCTAGATGATGCCTTAAAACTT
ACTGAACAACCAGAATTAGCAAATAAAGTAGACATGGTCTGGATAGTTGGTGGCAGTTCT
GTTTATAAGGAAGCCATGAATCACCCAGGCCATCTTAAACTATTTGTGACAAGGATCATG
CAAGACTTTGAAAGTGACACGTTTTTTCCAGAAATTGATTTGGAGAAATATAAACTTCTG
CCAGAATACCCAGGTGTTCTCTCTGATGTCCAGGAGGAGAAAGGCATTAAGTACAAATTT
GAAGTATATGAGAAGAATGATTAA
GenBank Gene IDJ00140
GeneCard IDNot Available
GenAtlas IDDHFR
HGNC IDHGNC:2861
Chromosome Location5
Locus5q11.2-q13.2
References
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  3. Yang JK, Masters JN, Attardi G: Human dihydrofolate reductase gene organization. Extensive conservation of the G + C-rich 5' non-coding sequence and strong intron size divergence from homologous mammalian genes. J Mol Biol. 1984 Jun 25;176(2):169-87. 6235374
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