Lysine-specific histone demethylase 1A

NameLysine-specific histone demethylase 1A
Synonyms
  • 1.-.-.-
  • AOF2
  • BRAF35-HDAC complex protein BHC110
  • Flavin-containing amine oxidase domain-containing protein 2
  • KDM1
  • KIAA0601
  • LSD1
Gene NameKDM1A
OrganismHuman
Amino acid sequence
>lcl|BSEQ0009316|Lysine-specific histone demethylase 1A
MLSGKKAAAAAAAAAAAATGTEAGPGTAGGSENGSEVAAQPAGLSGPAEVGPGAVGERTP
RKKEPPRASPPGGLAEPPGSAGPQAGPTVVPGSATPMETGIAETPEGRRTSRRKRAKVEY
REMDESLANLSEDEYYSEEERNAKAEKEKKLPPPPPQAPPEEENESEPEEPSGVEGAAFQ
SRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEA
PYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSF
GMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKC
PLYEANGQAVPKEKDEMVEQEFNRLLEATSYLSHQLDFNVLNNKPVSLGQALEVVIQLQE
KHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPPRDITAEFLV
KSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFAN
ATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGC
EVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLN
KVVLCFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISD
DVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPI
TPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQA
TPGVPAQQSPSM
Number of residues852
Molecular Weight92901.905
Theoretical pINot Available
GO Classification
Functions
  • transcription regulatory region DNA binding
  • histone demethylase activity (H3-K9 specific)
  • p53 binding
  • flavin adenine dinucleotide binding
  • histone demethylase activity
  • histone demethylase activity (H3-K4 specific)
  • histone demethylase activity (H3-dimethyl-K4 specific)
  • oxidoreductase activity
  • transcription factor activity, sequence-specific DNA binding
  • MRF binding
  • demethylase activity
  • chromatin binding
  • transcription factor binding
  • androgen receptor binding
  • ligand-dependent nuclear receptor transcription coactivator activity
  • enzyme binding
Processes
  • protein demethylation
  • cell proliferation
  • negative regulation of DNA binding
  • regulation of primitive erythrocyte differentiation
  • in utero embryonic development
  • histone H3-K9 demethylation
  • negative regulation of protein binding
  • positive regulation of stem cell proliferation
  • positive regulation of sequence-specific DNA binding transcription factor activity
  • positive regulation of transcription from RNA polymerase II promoter
  • negative regulation of transcription from RNA polymerase II promoter
  • regulation of cellular protein localization
  • negative regulation of DNA damage response, signal transduction by p53 class mediator
  • positive regulation of megakaryocyte differentiation
  • chromatin organization
  • transcription, DNA-templated
  • histone H3-K4 demethylation
  • regulation of transcription from RNA polymerase II promoter
  • granulocyte differentiation
  • blood coagulation
  • regulation of double-strand break repair via homologous recombination
  • negative regulation of sequence-specific DNA binding transcription factor activity
  • muscle cell development
  • negative regulation of histone H3-K4 methylation
  • negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
  • negative regulation of transcription, DNA-templated
  • positive regulation of histone ubiquitination
  • positive regulation of erythrocyte differentiation
  • pituitary gland development
  • positive regulation of hormone biosynthetic process
  • cellular response to UV
  • positive regulation of neural precursor cell proliferation
  • small GTPase mediated signal transduction
  • negative regulation of histone H3-K9 methylation
  • cellular response to gamma radiation
Components
  • transcription factor complex
  • nucleoplasm
  • nuclear chromatin
  • protein complex
  • nucleus
  • DNA repair complex
General FunctionTranscription regulatory region dna binding
Specific FunctionHistone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDO60341
UniProtKB Entry NameKDM1A_HUMAN
Cellular LocationNucleus
Gene sequence
>lcl|BSEQ0017747|Lysine-specific histone demethylase 1A (KDM1A)
ATGTTATCTGGGAAGAAGGCGGCAGCCGCGGCGGCGGCGGCTGCAGCGGCAGCAACCGGG
ACGGAGGCTGGCCCTGGGACAGCAGGCGGCTCCGAGAACGGGTCTGAGGTGGCCGCGCAG
CCCGCGGGCCTGTCGGGCCCAGCCGAGGTCGGGCCGGGGGCGGTGGGGGAGCGCACACCC
CGCAAGAAAGAGCCTCCGCGGGCCTCGCCCCCCGGGGGCCTGGCGGAACCGCCGGGGTCC
GCAGGGCCTCAGGCCGGCCCTACTGTCGTGCCTGGGTCTGCGACCCCCATGGAAACTGGA
ATAGCAGAGACTCCGGAGGGGCGTCGGACCAGCCGGCGCAAGCGGGCGAAGGTAGAGTAC
AGAGAGATGGATGAAAGCTTGGCCAACCTCTCAGAAGATGAGTATTATTCAGAAGAAGAG
AGAAATGCCAAAGCAGAGAAGGAAAAGAAGCTTCCCCCACCACCCCCTCAAGCCCCACCT
GAGGAAGAAAATGAAAGTGAGCCTGAAGAACCATCGGGGCAAGCAGGAGGACTTCAAGAC
GACAGTTCTGGAGGGTATGGAGACGGCCAAGCATCAGGTGTGGAGGGCGCAGCTTTCCAG
AGCCGACTTCCTCATGACCGGATGACTTCTCAAGAAGCAGCCTGTTTTCCAGATATTATC
AGTGGACCACAACAGACCCAGAAGGTTTTTCTTTTCATTAGAAACCGCACACTGCAGTTG
TGGTTGGATAATCCAAAGATTCAGCTGACATTTGAGGCTACTCTCCAACAATTAGAAGCA
CCTTATAACAGTGATACTGTGCTTGTCCACCGAGTTCACAGTTATTTAGAGCGTCATGGT
CTTATCAACTTCGGCATCTATAAGAGGATAAAACCCCTACCAACTAAAAAGACAGGAAAG
GTAATTATTATAGGCTCTGGGGTCTCAGGCTTGGCAGCAGCTCGACAGTTACAAAGTTTT
GGAATGGATGTCACACTTTTGGAAGCCAGGGATCGTGTGGGTGGACGAGTTGCCACATTT
CGCAAAGGAAACTATGTAGCTGATCTTGGAGCCATGGTGGTAACAGGTCTTGGAGGGAAT
CCTATGGCTGTGGTCAGCAAACAAGTAAATATGGAACTGGCCAAGATCAAGCAAAAATGC
CCACTTTATGAAGCCAACGGACAAGCTGACACTGTCAAGGTTCCTAAAGAGAAAGATGAA
ATGGTAGAGCAAGAGTTTAACCGGTTGCTAGAAGCTACATCTTACCTTAGTCATCAACTA
GACTTCAATGTCCTCAATAATAAGCCTGTGTCCCTTGGCCAGGCATTGGAAGTTGTCATT
CAGTTACAAGAGAAGCATGTCAAAGATGAGCAGATTGAACATTGGAAGAAGATAGTGAAA
ACTCAGGAAGAATTGAAAGAACTTCTTAATAAGATGGTAAATTTGAAAGAGAAAATTAAA
GAACTCCATCAGCAATACAAAGAAGCATCTGAAGTAAAGCCACCCAGAGATATTACTGCC
GAGTTCTTAGTGAAAAGCAAACACAGGGATCTGACCGCCCTATGCAAGGAATATGATGAA
TTAGCTGAAACACAAGGAAAGCTAGAAGAAAAACTTCAGGAGTTGGAAGCGAATCCCCCA
AGTGATGTATATCTCTCATCAAGAGACAGACAAATACTTGATTGGCATTTTGCAAATCTT
GAATTTGCTAATGCCACACCTCTCTCAACTCTCTCCCTTAAGCACTGGGATCAGGATGAT
GACTTTGAGTTCACTGGCAGCCACCTGACAGTAAGGAATGGCTACTCGTGTGTGCCTGTG
GCTTTAGCAGAAGGCCTAGACATTAAACTGAATACAGCAGTGCGACAGGTTCGCTACACG
GCTTCAGGATGTGAAGTGATAGCTGTGAATACCCGCTCCACGAGTCAAACCTTTATTTAT
AAATGCGACGCAGTTCTCTGTACCCTTCCCCTGGGTGTGCTGAAGCAGCAGCCACCAGCC
GTTCAGTTTGTGCCACCTCTCCCTGAGTGGAAAACATCTGCAGTCCAAAGGATGGGATTT
GGCAACCTTAACAAGGTGGTGTTGTGTTTTGATCGGGTGTTCTGGGATCCAAGTGTCAAT
TTGTTCGGGCATGTTGGCAGTACGACTGCCAGCAGGGGTGAGCTCTTCCTCTTCTGGAAC
CTCTATAAAGCTCCAATACTGTTGGCACTAGTGGCAGGAGAAGCTGCTGGTATCATGGAA
AACATAAGTGACGATGTGATTGTTGGCCGATGCCTGGCCATTCTCAAAGGGATTTTTGGT
AGCAGTGCAGTACCTCAGCCCAAAGAAACTGTGGTGTCTCGTTGGCGTGCTGATCCCTGG
GCTCGGGGCTCTTATTCCTATGTTGCTGCAGGATCATCTGGAAATGACTATGATTTAATG
GCTCAGCCAATCACTCCTGGCCCCTCGATTCCAGGTGCCCCACAGCCGATTCCACGACTC
TTCTTTGCGGGAGAACATACGATCCGTAACTACCCAGCCACAGTGCATGGTGCTCTGCTG
AGTGGGCTGCGAGAAGCGGGAAGAATTGCAGACCAGTTTTTGGGGGCCATGTATACGCTG
CCTCGCCAGGCCACACCAGGTGTTCCTGCACAGCAGTCCCCAAGCATGTGA
GenBank Gene IDNot Available
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:29079
Chromosome Location1
LocusNot Available
References
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