3-phosphoinositide-dependent protein kinase 1

Name3-phosphoinositide-dependent protein kinase 1
Synonyms
  • 2.7.11.1
  • hPDK1
  • PDK1
Gene NamePDPK1
OrganismHuman
Amino acid sequence
>lcl|BSEQ0002373|3-phosphoinositide-dependent protein kinase 1
MARTTSQLYDAVPIQSSVVLCSCPSPSMVRTQTESSTPPGIPGGSRQGPAMDGTAAEPRP
GAGSLQHAQPPPQPRKKRPEDFKFGKILGEGSFSTVVLARELATSREYAIKILEKRHIIK
ENKVPYVTRERDVMSRLDHPFFVKLYFTFQDDEKLYFGLSYAKNGELLKYIRKIGSFDET
CTRFYTAEIVSALEYLHGKGIIHRDLKPENILLNEDMHIQITDFGTAKVLSPESKQARAN
SFVGTAQYVSPELLTEKSACKSSDLWALGCIIYQLVAGLPPFRAGNEYLIFQKIIKLEYD
FPEKFFPKARDLVEKLLVLDATKRLGCEEMEGYGPLKAHPFFESVTWENLHQQTPPKLTA
YLPAMSEDDEDCYGNYDNLLSQFGCMQVSSSSSSHSLSASDTGLPQRSGSNIEQYIHDLD
SNSFELDLQFSEDEKRLLLEKQAGGNPWHQFVENNLILKMGPVDKRKGLFARRRQLLLTE
GPHLYYVDPVNKVLKGEIPWSQELRPEAKNFKTFFVHTPNRTYYLMDPSGNAHKWCRKIQ
EVWRQRYQSHPDAAVQ
Number of residues556
Molecular Weight63151.305
Theoretical pI7.38
GO Classification
Functions
  • phospholipase activator activity
  • ATP binding
  • protein serine/threonine kinase activity
  • 3-phosphoinositide-dependent protein kinase activity
  • phospholipase binding
Processes
  • Fc-epsilon receptor signaling pathway
  • regulation of mast cell degranulation
  • protein autophosphorylation
  • cellular response to epidermal growth factor stimulus
  • transcription, DNA-templated
  • fibroblast growth factor receptor signaling pathway
  • type B pancreatic cell development
  • peptidyl-threonine phosphorylation
  • insulin receptor signaling pathway
  • negative regulation of cardiac muscle cell apoptotic process
  • blood coagulation
  • T cell costimulation
  • neurotrophin TRK receptor signaling pathway
  • T cell receptor signaling pathway
  • protein phosphorylation
  • regulation of endothelial cell migration
  • small GTPase mediated signal transduction
  • stimulatory C-type lectin receptor signaling pathway
  • synaptic transmission
  • activation of protein kinase B activity
  • vascular endothelial growth factor receptor signaling pathway
  • platelet activation
  • positive regulation of phospholipase activity
  • positive regulation of release of sequestered calcium ion into cytosol
  • negative regulation of protein kinase activity
  • focal adhesion assembly
  • intracellular signal transduction
  • extrinsic apoptotic signaling pathway
  • calcium-mediated signaling
  • regulation of I-kappaB kinase/NF-kappaB signaling
  • hyperosmotic response
  • cellular response to insulin stimulus
  • actin cytoskeleton organization
  • cell migration
  • positive regulation of establishment of protein localization to plasma membrane
  • epidermal growth factor receptor signaling pathway
  • negative regulation of toll-like receptor signaling pathway
  • innate immune response
  • regulation of transcription, DNA-templated
  • negative regulation of transforming growth factor beta receptor signaling pathway
  • phosphatidylinositol-mediated signaling
Components
  • cytoplasm
  • plasma membrane
  • cell projection
  • cytosol
  • nucleoplasm
  • cytoplasmic membrane-bounded vesicle
  • neuronal postsynaptic density
  • focal adhesion
General FunctionProtein serine/threonine kinase activity
Specific FunctionSerine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein ID2407613
UniProtKB IDO15530
UniProtKB Entry NamePDPK1_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0021289|3-phosphoinositide-dependent protein kinase 1 (PDPK1)
ATGGCCAGGACCACCAGCCAGCTGTATGACGCCGTGCCCATCCAGTCCAGCGTGGTGTTA
TGTTCCTGCCCATCCCCATCAATGGTGAGGACCCAGACTGAGTCCAGCACGCCCCCTGGC
ATTCCTGGTGGCAGCAGGCAGGGCCCCGCCATGGACGGCACTGCAGCCGAGCCTCGGCCC
GGCGCCGGCTCCCTGCAGCATGCCCAGCCTCCGCCGCAGCCTCGGAAGAAGCGGCCTGAG
GACTTCAAGTTTGGGAAAATCCTTGGGGAAGGCTCTTTTTCCACGGTTGTCCTGGCTCGA
GAACTGGCAACCTCCAGAGAATATGCGATTAAAATTCTGGAGAAGCGACATATCATAAAA
GAGAACAAGGTCCCCTATGTAACCAGAGAGCGGGATGTCATGTCGCGCCTGGATCACCCC
TTCTTTGTTAAGCTTTACTTCACATTTCAGGACGACGAGAAGCTGTATTTCGGCCTTAGT
TATGCCAAAAATGGAGAACTACTTAAATATATTCGCAAAATCGGTTCATTCGATGAGACC
TGTACCCGATTTTACACGGCTGAGATTGTGTCTGCTTTAGAGTACTTGCACGGCAAGGGC
ATCATTCACAGGGACCTTAAACCGGAAAACATTTTGTTAAATGAAGATATGCACATCCAG
ATCACAGATTTTGGAACAGCAAAAGTCTTATCCCCAGAGAGCAAACAAGCCAGGGCCAAC
TCATTCGTGGGAACAGCGCAGTACGTTTCTCCAGAGCTGCTCACGGAGAAGTCCGCCTGT
AAGAGTTCAGACCTTTGGGCTCTTGGATGCATAATATACCAGCTTGTGGCAGGACTCCCA
CCATTCCGAGCTGGAAACGAGTATCTTATATTTCAGAAGATCATTAAGTTGGAATATGAC
TTTCCAGAAAAATTCTTCCCTAAGGCAAGAGACCTCGTGGAGAAACTTTTGGTTTTAGAT
GCCACAAAGCGGTTAGGCTGTGAGGAAATGGAAGGATACGGACCTCTTAAAGCACACCCG
TTCTTCGAGTCCGTCACGTGGGAGAACCTGCACCAGCAGACGCCTCCGAAGCTCACCGCT
TACCTGCCGGCTATGTCGGAAGACGACGAGGACTGCTATGGCAATTATGACAATCTCCTG
AGCCAGTTTGGCTGCATGCAGGTGTCTTCGTCCTCCTCCTCACACTCCCTGTCAGCCTCC
GACACGGGCCTGCCCCAGAGGTCAGGCAGCAACATAGAGCAGTACATTCACGATCTGGAC
TCGAACTCCTTTGAACTGGACTTACAGTTTTCCGAAGATGAGAAGAGGTTGTTGTTGGAG
AAGCAGGCTGGCGGAAACCCTTGCCTAACAGGACGTATTATCTGA
GenBank Gene IDAF017995
GeneCard IDNot Available
GenAtlas IDPDPK1
HGNC IDHGNC:8816
Chromosome Location16
Locus16p13.3
References
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