DNA-directed RNA polymerase I subunit RPA34

NameDNA-directed RNA polymerase I subunit RPA34
Synonyms
  • A34.5
  • Antisense to ERCC-1 protein
  • ASE-1
  • ASE1
  • CAST
  • CD3-epsilon-associated protein
  • CD3E-associated protein
  • PAF49
  • RNA polymerase I-associated factor PAF49
Gene NameCD3EAP
OrganismHuman
Amino acid sequence
>lcl|BSEQ0009194|DNA-directed RNA polymerase I subunit RPA34
MEEPQAGDAARFSCPPNFTAKPPASESPRFSLEALTGPDTELWLIQAPADFAPECFNGRH
VPLSGSQIVKGKLAGKRHRYRVLSSCPQAGEATLLAPSTEAGGGLTCASAPQGTLRILEG
PQQSLSGSPLQPIPASPPPQIPPGLRPRFCAFGGNPPVTGPRSALAPNLLTSGKKKKEMQ
VTEAPVTQEAVNGHGALEVDMALGSPEMDVRKKKKKKNQQLKEPEAAGPVGTEPTVETLE
PLGVLFPSTTKKRKKPKGKETFEPEDKTVKQEQINTEPLEDTVLSPTKKRKRQKGTEGME
PEEGVTVESQPQVKVEPLEEAIPLPPTKKRKKEKGQMAMMEPGTEAMEPVEPEMKPLESP
GGTMAPQQPEGAKPQAQAALAAPKKKTKKEKQQDATVEPETEVVGPELPDDLEPQAAPTS
TKKKKKKKERGHTVTEPIQPLEPELPGEGQPEARATPGSTKKRKKQSQESRMPETVPQEE
MPGPPLNSESGEEAPTGRDKKRKQQQQQPV
Number of residues510
Molecular Weight54985.335
Theoretical pINot Available
GO Classification
Functions
  • DNA-directed RNA polymerase activity
  • poly(A) RNA binding
Processes
  • transcription elongation from RNA polymerase I promoter
  • transcription from RNA polymerase I promoter
  • transcription initiation from RNA polymerase I promoter
  • transmembrane receptor protein tyrosine kinase signaling pathway
  • gene expression
  • negative regulation of gene expression, epigenetic
  • regulation of gene expression, epigenetic
  • rRNA transcription
  • termination of RNA polymerase I transcription
Components
  • cytoplasm
  • mitochondrion
  • RNA polymerase I transcription factor complex
  • nucleus
  • chromosome
  • nucleolus
  • nucleoplasm
  • DNA-directed RNA polymerase I complex
General FunctionPoly(a) rna binding
Specific FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
Pfam Domain Function
Transmembrane RegionsNot Available
GenBank Protein IDNot Available
UniProtKB IDO15446
UniProtKB Entry NameRPA34_HUMAN
Cellular LocationNucleus
Gene sequence
>lcl|BSEQ0017715|DNA-directed RNA polymerase I subunit RPA34 (CD3EAP)
ATGGAGGAGCCCCAGGCCGGCGGTGAGGATGCTGCTCGGTTCTCTTGTCCCCCCAACTTT
ACCGCGAAGCCCCCAGCCTCAGAGTCCCCTCGTTTCTCCTTGGAGGCGCTGACGGGTCCA
GATACGGAGCTGTGGCTTATTCAGGCCCCTGCAGACTTTGCCCCAGAATGCTTCAATGGG
CGGCATGTGCCTCTCTCTGGCTCCCAGATCGTCAAGGGCAAATTGGCAGGCAAGCGGCAC
CGCTATCGAGTCCTCAGCAGCTGTCCCCAAGCTGGAGAAGCGACCCTGCTGGCCCCCTCA
ACGGAGGCAGGAGGTGGACTCACCTGTGCCTCAGCCCCCCAGGGCACCCTAAGGATCCTT
GAGGGTCCCCAGCAATCCCTGTCAGGGAGCCCTCTGCAGCCCATCCCAGCAAGTCCCCCA
CCACAGATCCCTCCTGGCCTGAGGCCTCGGTTCTGTGCCTTTGGGGGCAACCCACCAGTC
ACAGGGCCTAGGTCAGCCTTGGCCCCCAACCTGCTCACCTCAGGGAAGAAGAAAAAGGAG
ATGCAGGTGACAGAGGCCCCAGTCACTCAGGAGGCAGTGAATGGGCACGGGGCCCTGGAG
GTGGACATGGCTTTGGGGTCGCCAGAAATGGATGTGCGGAAGAAGAAGAAGAAAAAAAAT
CAGCAGCTGAAAGAACCAGAGGCAGCAGGGCCTGTGGGGACAGAGCCCACAGTGGAGACA
CTGGAGCCTCTGGGAGTGCTGTTCCCGTCCACCACCAAGAAGAGGAAGAAGCCCAAAGGG
AAAGAAACCTTCGAGCCAGAAGACAAGACAGTGAAGCAGGAACAGATTAACACTGAGCCT
CTAGAAGACACAGTCCTGTCCCCGACCAAAAAGAGAAAGAGGCAAAAGGGGACGGAAGGG
ATGGAGCCAGAGGAGGGGGTGACAGTTGAGTCTCAGCCACAGGTGAAGGTGGAGCCACTG
GAGGAAGCCATCCCTCTGCCCCCTACGAAGAAGAGGAAAAAAGAAAAGGGACAGATGGCA
ATGATGGAGCCAGGGACGGAGGCGATGGAGCCAGTGGAGCCGGAGATGAAGCCTCTGGAG
TCCCCAGGGGGGACCATGGCGCCTCAACAGCCAGAAGGAGCGAAGCCTCAGGCCCAGGCA
GCTCTGGCAGCTCCCAAAAAGAAGACGAAGAAAGAAAAACAGCAAGATGCCACAGTGGAG
CCAGAGACAGAGGTGGTGGGGCCTGAGCTGCCGGATGACCTTGAGCCTCAGGCAGCTCCC
ACATCCACCAAGAAGAAGAAGAAGAAGAAAGAGAGAGGTCACACAGTGACTGAGCCAATT
CAGCCACTAGAGCCTGAACTGCCAGGGGAGGGACAGCCTGAAGCCAGGGCAACTCCGGGA
TCCACCAAGAAGAGGAAGAAGCAGAGTCAGGAAAGCCGGATGCCAGAGACAGTGCCCCAA
GAGGAGATGCCAGGGCCGCCACTGAATTCAGAGTCTGGGGAGGAGGCTCCCACAGGCCGG
GACAAGAAGCGGAAGCAGCAGCAGCAGCAGCCTGTGTAG
GenBank Gene IDNot Available
GeneCard IDNot Available
GenAtlas IDNot Available
HGNC IDHGNC:24219
Chromosome Location19
LocusNot Available
References
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  2. Yamazaki T, Hamano Y, Tashiro H, Itoh K, Nakano H, Miyatake S, Saito T: CAST, a novel CD3epsilon-binding protein transducing activation signal for interleukin-2 production in T cells. J Biol Chem. 1999 Jun 25;274(26):18173-80. 10373416
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